Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF494 and GFF495 are separated by 157 nucleotides GFF495 and GFF496 are separated by 261 nucleotides
GFF494 - Protein yhjK, at 219,358 to 221,364
GFF494
GFF495 - Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate, at 221,522 to 222,808
GFF495
GFF496 - Protein YhjJ, putative peptidase, at 223,070 to 224,515
GFF496
Position (kb) 221
222
223 Strain fitness (log2 ratio) -2
-1
0
1 at 220.701 kb on + strand, within GFF494 at 220.702 kb on - strand, within GFF494 at 220.702 kb on - strand, within GFF494 at 220.702 kb on - strand, within GFF494 at 220.702 kb on - strand, within GFF494 at 221.005 kb on - strand, within GFF494 at 221.009 kb on + strand, within GFF494 at 221.009 kb on + strand, within GFF494 at 221.019 kb on - strand, within GFF494 at 221.066 kb on - strand, within GFF494 at 221.085 kb on - strand, within GFF494 at 221.092 kb on - strand, within GFF494 at 221.200 kb on - strand at 221.205 kb on - strand at 221.353 kb on + strand at 221.597 kb on + strand at 221.598 kb on - strand at 221.601 kb on - strand at 221.656 kb on + strand, within GFF495 at 221.657 kb on - strand, within GFF495 at 221.800 kb on + strand, within GFF495 at 221.908 kb on + strand, within GFF495 at 222.153 kb on - strand, within GFF495 at 222.153 kb on - strand, within GFF495 at 222.191 kb on - strand, within GFF495 at 222.200 kb on + strand, within GFF495 at 222.201 kb on - strand, within GFF495 at 222.201 kb on - strand, within GFF495 at 222.201 kb on - strand, within GFF495 at 222.202 kb on + strand, within GFF495 at 222.212 kb on - strand, within GFF495 at 222.284 kb on + strand, within GFF495 at 222.285 kb on - strand, within GFF495 at 222.440 kb on - strand, within GFF495 at 222.490 kb on + strand, within GFF495 at 222.510 kb on + strand, within GFF495 at 222.510 kb on + strand, within GFF495 at 222.592 kb on + strand, within GFF495 at 222.655 kb on + strand, within GFF495 at 222.655 kb on + strand, within GFF495 at 222.736 kb on - strand at 222.764 kb on - strand at 222.819 kb on + strand at 222.819 kb on + strand at 222.860 kb on - strand at 222.860 kb on - strand at 222.883 kb on + strand at 222.883 kb on + strand at 222.883 kb on + strand at 222.883 kb on + strand at 222.884 kb on - strand at 222.884 kb on - strand at 222.897 kb on + strand at 222.961 kb on - strand at 222.967 kb on + strand at 223.089 kb on - strand at 223.089 kb on - strand at 223.196 kb on - strand at 223.216 kb on + strand, within GFF496 at 223.395 kb on - strand, within GFF496 at 223.449 kb on - strand, within GFF496 at 223.468 kb on + strand, within GFF496 at 223.519 kb on + strand, within GFF496 at 223.520 kb on - strand, within GFF496 at 223.546 kb on + strand, within GFF496 at 223.547 kb on - strand, within GFF496
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 220,701 + GFF494 0.67 +0.1 220,702 - GFF494 0.67 -0.0 220,702 - GFF494 0.67 -1.6 220,702 - GFF494 0.67 +0.4 220,702 - GFF494 0.67 +0.2 221,005 - GFF494 0.82 -0.3 221,009 + GFF494 0.82 -0.2 221,009 + GFF494 0.82 -0.0 221,019 - GFF494 0.83 +0.1 221,066 - GFF494 0.85 -0.1 221,085 - GFF494 0.86 +0.3 221,092 - GFF494 0.86 +0.1 221,200 - -0.6 221,205 - +0.5 221,353 + -1.4 221,597 + -0.8 221,598 - -0.4 221,601 - -0.9 221,656 + GFF495 0.10 -0.6 221,657 - GFF495 0.10 -1.0 221,800 + GFF495 0.22 +0.5 221,908 + GFF495 0.30 -2.0 222,153 - GFF495 0.49 -0.2 222,153 - GFF495 0.49 -1.0 222,191 - GFF495 0.52 -0.8 222,200 + GFF495 0.53 -0.2 222,201 - GFF495 0.53 -1.1 222,201 - GFF495 0.53 +0.4 222,201 - GFF495 0.53 -0.8 222,202 + GFF495 0.53 -1.9 222,212 - GFF495 0.54 -1.0 222,284 + GFF495 0.59 -0.9 222,285 - GFF495 0.59 -0.8 222,440 - GFF495 0.71 -0.5 222,490 + GFF495 0.75 -0.2 222,510 + GFF495 0.77 +0.0 222,510 + GFF495 0.77 -0.5 222,592 + GFF495 0.83 +0.9 222,655 + GFF495 0.88 -0.0 222,655 + GFF495 0.88 -0.3 222,736 - +0.6 222,764 - -0.5 222,819 + -0.4 222,819 + -0.4 222,860 - +0.2 222,860 - -0.1 222,883 + +0.2 222,883 + +0.4 222,883 + +0.2 222,883 + +0.2 222,884 - -0.0 222,884 - -0.1 222,897 + +0.3 222,961 - +0.2 222,967 + -0.0 223,089 - +0.1 223,089 - +0.0 223,196 - +0.3 223,216 + GFF496 0.10 +0.2 223,395 - GFF496 0.22 +0.7 223,449 - GFF496 0.26 +0.2 223,468 + GFF496 0.28 -0.1 223,519 + GFF496 0.31 +0.4 223,520 - GFF496 0.31 +1.1 223,546 + GFF496 0.33 +0.1 223,547 - GFF496 0.33 -0.2
Or see this region's nucleotide sequence