Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4904 and GFF4905 overlap by 4 nucleotides GFF4905 and GFF4906 are separated by 145 nucleotides
GFF4904 - Macrolide export ATP-binding/permease protein MacB (EC 3.6.3.-), at 3,404 to 5,350
GFF4904
GFF4905 - Macrolide-specific efflux protein MacA, at 5,347 to 6,465
GFF4905
GFF4906 - Virulence factor VirK, at 6,611 to 7,561
GFF4906
Position (kb) 5
6
7 Strain fitness (log2 ratio) -1
0
1 at 4.354 kb on - strand, within GFF4904 at 4.423 kb on + strand, within GFF4904 at 4.504 kb on + strand, within GFF4904 at 4.505 kb on - strand, within GFF4904 at 4.532 kb on + strand, within GFF4904 at 4.627 kb on + strand, within GFF4904 at 4.627 kb on + strand, within GFF4904 at 4.631 kb on + strand, within GFF4904 at 4.632 kb on - strand, within GFF4904 at 4.765 kb on + strand, within GFF4904 at 4.765 kb on + strand, within GFF4904 at 4.766 kb on - strand, within GFF4904 at 4.766 kb on - strand, within GFF4904 at 5.014 kb on - strand, within GFF4904 at 5.080 kb on + strand, within GFF4904 at 5.195 kb on - strand at 5.311 kb on - strand at 5.314 kb on + strand at 5.314 kb on + strand at 5.315 kb on - strand at 5.494 kb on - strand, within GFF4905 at 5.494 kb on - strand, within GFF4905 at 5.571 kb on + strand, within GFF4905 at 5.572 kb on - strand, within GFF4905 at 5.573 kb on + strand, within GFF4905 at 5.574 kb on - strand, within GFF4905 at 5.974 kb on + strand, within GFF4905 at 5.974 kb on + strand, within GFF4905 at 5.974 kb on + strand, within GFF4905 at 5.974 kb on + strand, within GFF4905 at 5.975 kb on - strand, within GFF4905 at 5.975 kb on - strand, within GFF4905 at 6.054 kb on + strand, within GFF4905 at 6.059 kb on - strand, within GFF4905 at 6.097 kb on + strand, within GFF4905 at 6.200 kb on - strand, within GFF4905 at 6.214 kb on - strand, within GFF4905 at 6.377 kb on - strand at 6.378 kb on - strand at 6.592 kb on + strand at 6.593 kb on - strand at 6.593 kb on - strand at 6.650 kb on + strand at 6.651 kb on - strand at 6.757 kb on - strand, within GFF4906 at 6.813 kb on + strand, within GFF4906 at 6.868 kb on + strand, within GFF4906 at 6.876 kb on + strand, within GFF4906 at 7.303 kb on + strand, within GFF4906 at 7.303 kb on + strand, within GFF4906 at 7.336 kb on + strand, within GFF4906 at 7.337 kb on - strand, within GFF4906 at 7.427 kb on + strand, within GFF4906 at 7.427 kb on + strand, within GFF4906 at 7.432 kb on + strand, within GFF4906
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 4,354 - GFF4904 0.49 +0.8 4,423 + GFF4904 0.52 -0.2 4,504 + GFF4904 0.56 -1.0 4,505 - GFF4904 0.57 -0.1 4,532 + GFF4904 0.58 -0.2 4,627 + GFF4904 0.63 -0.8 4,627 + GFF4904 0.63 +0.2 4,631 + GFF4904 0.63 +0.4 4,632 - GFF4904 0.63 +0.3 4,765 + GFF4904 0.70 -1.4 4,765 + GFF4904 0.70 +0.1 4,766 - GFF4904 0.70 -0.2 4,766 - GFF4904 0.70 -0.3 5,014 - GFF4904 0.83 +0.2 5,080 + GFF4904 0.86 +0.6 5,195 - +0.4 5,311 - -0.4 5,314 + -0.6 5,314 + +0.2 5,315 - +0.7 5,494 - GFF4905 0.13 -0.4 5,494 - GFF4905 0.13 +0.3 5,571 + GFF4905 0.20 +1.4 5,572 - GFF4905 0.20 -0.0 5,573 + GFF4905 0.20 +0.4 5,574 - GFF4905 0.20 +0.1 5,974 + GFF4905 0.56 +0.7 5,974 + GFF4905 0.56 +0.7 5,974 + GFF4905 0.56 +0.1 5,974 + GFF4905 0.56 -0.2 5,975 - GFF4905 0.56 +0.3 5,975 - GFF4905 0.56 +0.3 6,054 + GFF4905 0.63 +0.1 6,059 - GFF4905 0.64 +1.3 6,097 + GFF4905 0.67 -0.8 6,200 - GFF4905 0.76 +0.0 6,214 - GFF4905 0.77 +0.4 6,377 - +0.5 6,378 - +0.5 6,592 + -0.9 6,593 - +0.7 6,593 - +0.3 6,650 + -0.8 6,651 - +0.4 6,757 - GFF4906 0.15 -1.2 6,813 + GFF4906 0.21 +0.5 6,868 + GFF4906 0.27 +0.5 6,876 + GFF4906 0.28 -0.6 7,303 + GFF4906 0.73 +0.6 7,303 + GFF4906 0.73 +0.0 7,336 + GFF4906 0.76 +0.5 7,337 - GFF4906 0.76 +0.3 7,427 + GFF4906 0.86 +0.3 7,427 + GFF4906 0.86 +0.3 7,432 + GFF4906 0.86 +0.1
Or see this region's nucleotide sequence