Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4883 and GFF4884 are separated by 170 nucleotides GFF4884 and GFF4899 are separated by 100 nucleotides GFF4899 and GFF4885 are separated by 148 nucleotides
GFF4883 - Mobile element protein, at 409 to 1,092
GFF4883
GFF4884 - Mobile element protein, at 1,263 to 1,664
GFF4884
GFF4899 - tRNA-Ser-GGA, at 1,765 to 1,852
GFF4899
GFF4885 - Nicotinamidase family protein YcaC, at 2,001 to 2,678
GFF4885
Position (kb) 0
1
2 Strain fitness (log2 ratio) -1
0
1 at 0.048 kb on + strand at 0.116 kb on + strand at 0.117 kb on - strand at 0.169 kb on - strand at 0.392 kb on + strand at 0.422 kb on - strand at 0.510 kb on - strand, within GFF4883 at 0.775 kb on + strand, within GFF4883 at 0.888 kb on + strand, within GFF4883 at 0.888 kb on + strand, within GFF4883 at 0.888 kb on + strand, within GFF4883 at 0.889 kb on - strand, within GFF4883 at 0.889 kb on - strand, within GFF4883 at 0.957 kb on + strand, within GFF4883 at 1.002 kb on - strand, within GFF4883 at 1.002 kb on - strand, within GFF4883 at 1.195 kb on - strand at 1.203 kb on + strand at 1.258 kb on - strand at 1.508 kb on - strand, within GFF4884 at 1.511 kb on + strand, within GFF4884 at 1.511 kb on + strand, within GFF4884 at 1.527 kb on + strand, within GFF4884 at 1.528 kb on - strand, within GFF4884 at 1.573 kb on + strand, within GFF4884 at 1.617 kb on - strand, within GFF4884 at 1.854 kb on + strand at 1.901 kb on - strand at 1.962 kb on + strand at 1.963 kb on - strand at 1.968 kb on + strand at 1.969 kb on - strand at 2.077 kb on + strand, within GFF4885 at 2.088 kb on + strand, within GFF4885
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 48 + -0.1 116 + +0.8 117 - -0.5 169 - +0.2 392 + -0.2 422 - +0.4 510 - GFF4883 0.15 +0.6 775 + GFF4883 0.54 +0.4 888 + GFF4883 0.70 +0.5 888 + GFF4883 0.70 +0.4 888 + GFF4883 0.70 +0.2 889 - GFF4883 0.70 +0.6 889 - GFF4883 0.70 +0.0 957 + GFF4883 0.80 +0.7 1,002 - GFF4883 0.87 -0.5 1,002 - GFF4883 0.87 +0.3 1,195 - +0.8 1,203 + -0.8 1,258 - +0.0 1,508 - GFF4884 0.61 +0.4 1,511 + GFF4884 0.62 -0.7 1,511 + GFF4884 0.62 +0.3 1,527 + GFF4884 0.66 -1.0 1,528 - GFF4884 0.66 +0.6 1,573 + GFF4884 0.77 +0.1 1,617 - GFF4884 0.88 -0.6 1,854 + -0.5 1,901 - -0.6 1,962 + +0.3 1,963 - -0.0 1,968 + +0.7 1,969 - +1.5 2,077 + GFF4885 0.11 -0.1 2,088 + GFF4885 0.13 +0.5
Or see this region's nucleotide sequence