Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4846 and GFF4847 overlap by 1 nucleotides GFF4847 and GFF4848 are separated by 611 nucleotides GFF4848 and GFF4849 are separated by 68 nucleotides
GFF4846 - tRNA pseudouridine synthase C (EC 4.2.1.70), at 145,510 to 146,292
GFF4846
GFF4847 - Hypothetical protein YqcC (clustered with tRNA pseudouridine synthase C), at 146,292 to 146,621
GFF4847
GFF4848 - Syd protein, at 147,233 to 147,778
GFF4848
GFF4849 - NADPH dependent preQ0 reductase (EC 1.7.1.13), at 147,847 to 148,695
GFF4849
Position (kb)
147
148 Strain fitness (log2 ratio)
-2
-1
0
1 at 146.277 kb on - strand at 146.300 kb on + strand at 146.389 kb on + strand, within GFF4847 at 146.390 kb on - strand, within GFF4847 at 146.447 kb on + strand, within GFF4847 at 146.447 kb on + strand, within GFF4847 at 146.447 kb on + strand, within GFF4847 at 146.448 kb on - strand, within GFF4847 at 146.448 kb on - strand, within GFF4847 at 146.448 kb on - strand, within GFF4847 at 146.585 kb on + strand, within GFF4847 at 146.585 kb on + strand, within GFF4847 at 146.586 kb on - strand, within GFF4847 at 146.586 kb on - strand, within GFF4847 at 146.586 kb on - strand, within GFF4847 at 146.953 kb on - strand at 147.032 kb on - strand at 147.057 kb on - strand at 147.289 kb on + strand, within GFF4848 at 147.480 kb on - strand, within GFF4848 at 147.484 kb on + strand, within GFF4848 at 147.560 kb on + strand, within GFF4848 at 147.561 kb on - strand, within GFF4848 at 147.561 kb on - strand, within GFF4848 at 147.566 kb on - strand, within GFF4848 at 147.630 kb on + strand, within GFF4848 at 147.672 kb on - strand, within GFF4848 at 147.672 kb on - strand, within GFF4848 at 147.807 kb on - strand at 147.819 kb on + strand at 147.824 kb on + strand at 148.024 kb on + strand, within GFF4849 at 148.101 kb on + strand, within GFF4849 at 148.325 kb on - strand, within GFF4849 at 148.376 kb on + strand, within GFF4849 at 148.522 kb on - strand, within GFF4849 at 148.544 kb on + strand, within GFF4849 at 148.610 kb on - strand, within GFF4849 at 148.731 kb on + strand at 148.731 kb on + strand at 148.732 kb on - strand at 148.761 kb on - strand at 148.765 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 146,277 - +0.0 146,300 + -0.1 146,389 + GFF4847 0.29 -1.2 146,390 - GFF4847 0.30 -0.3 146,447 + GFF4847 0.47 -0.5 146,447 + GFF4847 0.47 -0.0 146,447 + GFF4847 0.47 +0.3 146,448 - GFF4847 0.47 -0.1 146,448 - GFF4847 0.47 -0.4 146,448 - GFF4847 0.47 +0.2 146,585 + GFF4847 0.89 +0.4 146,585 + GFF4847 0.89 -1.5 146,586 - GFF4847 0.89 +1.1 146,586 - GFF4847 0.89 -0.1 146,586 - GFF4847 0.89 -0.3 146,953 - +0.2 147,032 - +0.8 147,057 - -1.5 147,289 + GFF4848 0.10 -0.1 147,480 - GFF4848 0.45 +0.5 147,484 + GFF4848 0.46 +0.3 147,560 + GFF4848 0.60 -0.1 147,561 - GFF4848 0.60 +0.2 147,561 - GFF4848 0.60 +0.1 147,566 - GFF4848 0.61 -0.1 147,630 + GFF4848 0.73 +0.1 147,672 - GFF4848 0.80 +0.6 147,672 - GFF4848 0.80 +0.4 147,807 - +0.2 147,819 + -0.2 147,824 + -0.9 148,024 + GFF4849 0.21 -0.1 148,101 + GFF4849 0.30 -0.2 148,325 - GFF4849 0.56 +0.3 148,376 + GFF4849 0.62 -1.9 148,522 - GFF4849 0.80 +0.4 148,544 + GFF4849 0.82 -1.8 148,610 - GFF4849 0.90 -0.4 148,731 + -0.3 148,731 + +0.2 148,732 - +0.3 148,761 - -0.2 148,765 + +0.5
Or see this region's nucleotide sequence