Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF480 and GFF481 are separated by 117 nucleotides GFF481 and GFF482 overlap by 4 nucleotides GFF482 and GFF483 are separated by 103 nucleotides GFF483 and GFF484 are separated by 81 nucleotides GFF484 and GFF485 overlap by 4 nucleotides GFF485 and GFF486 overlap by 4 nucleotides
GFF480 - Serine transporter, at 202,849 to 204,147
GFF480
GFF481 - hypothetical protein, at 204,265 to 204,405
GFF481
GFF482 - FIG01047459: hypothetical protein, at 204,402 to 204,662
GFF482
GFF483 - hypothetical protein, at 204,766 to 204,897
GFF483
GFF484 - FIG002337: predicted inner membrane protein, at 204,979 to 206,658
GFF484
GFF485 - FIG005119: putative inner membrane protein, at 206,655 to 206,846
GFF485
GFF486 - FIG005274: hypothetical protein, at 206,843 to 208,402
GFF486
Position (kb) 204
205
206
207 Strain fitness (log2 ratio) -1
0
1 at 204.011 kb on - strand, within GFF480 at 204.147 kb on + strand at 204.184 kb on - strand at 204.197 kb on + strand at 204.198 kb on - strand at 204.210 kb on + strand at 204.211 kb on - strand at 204.398 kb on + strand at 204.398 kb on + strand at 204.399 kb on - strand at 204.464 kb on - strand, within GFF482 at 204.512 kb on + strand, within GFF482 at 204.663 kb on - strand at 204.701 kb on + strand at 204.704 kb on - strand at 204.789 kb on + strand, within GFF483 at 204.789 kb on + strand, within GFF483 at 204.798 kb on + strand, within GFF483 at 205.022 kb on + strand at 205.022 kb on + strand at 205.022 kb on + strand at 205.030 kb on - strand at 205.057 kb on - strand at 205.138 kb on - strand at 205.195 kb on - strand, within GFF484 at 205.195 kb on - strand, within GFF484 at 205.348 kb on + strand, within GFF484 at 205.349 kb on - strand, within GFF484 at 205.359 kb on + strand, within GFF484 at 205.430 kb on + strand, within GFF484 at 205.489 kb on - strand, within GFF484 at 205.566 kb on - strand, within GFF484 at 205.635 kb on + strand, within GFF484 at 205.635 kb on + strand, within GFF484 at 205.635 kb on + strand, within GFF484 at 205.830 kb on - strand, within GFF484 at 205.880 kb on - strand, within GFF484 at 205.935 kb on + strand, within GFF484 at 206.018 kb on + strand, within GFF484 at 206.019 kb on - strand, within GFF484 at 206.219 kb on - strand, within GFF484 at 206.263 kb on + strand, within GFF484 at 206.386 kb on - strand, within GFF484 at 206.486 kb on - strand, within GFF484 at 206.609 kb on + strand at 206.709 kb on + strand, within GFF485 at 206.710 kb on - strand, within GFF485 at 206.731 kb on - strand, within GFF485 at 206.745 kb on - strand, within GFF485 at 206.928 kb on - strand at 206.987 kb on + strand at 206.988 kb on - strand at 207.079 kb on + strand, within GFF486 at 207.157 kb on + strand, within GFF486 at 207.158 kb on - strand, within GFF486 at 207.186 kb on - strand, within GFF486 at 207.192 kb on - strand, within GFF486 at 207.283 kb on + strand, within GFF486 at 207.284 kb on - strand, within GFF486 at 207.413 kb on - strand, within GFF486 at 207.511 kb on + strand, within GFF486 at 207.604 kb on + strand, within GFF486
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 204,011 - GFF480 0.89 -0.3 204,147 + +0.2 204,184 - -0.3 204,197 + -0.8 204,198 - +0.5 204,210 + +0.9 204,211 - -0.3 204,398 + -0.1 204,398 + +0.2 204,399 - -0.7 204,464 - GFF482 0.24 -0.0 204,512 + GFF482 0.42 -0.4 204,663 - +0.5 204,701 + +0.0 204,704 - -0.1 204,789 + GFF483 0.17 +0.3 204,789 + GFF483 0.17 +1.5 204,798 + GFF483 0.24 +0.3 205,022 + +0.4 205,022 + +0.6 205,022 + +0.2 205,030 - +0.0 205,057 - +0.3 205,138 - -0.7 205,195 - GFF484 0.13 +1.1 205,195 - GFF484 0.13 +0.3 205,348 + GFF484 0.22 +0.4 205,349 - GFF484 0.22 +0.5 205,359 + GFF484 0.23 +0.0 205,430 + GFF484 0.27 +0.0 205,489 - GFF484 0.30 +0.3 205,566 - GFF484 0.35 +0.0 205,635 + GFF484 0.39 -1.1 205,635 + GFF484 0.39 +0.5 205,635 + GFF484 0.39 +0.9 205,830 - GFF484 0.51 -0.1 205,880 - GFF484 0.54 -0.1 205,935 + GFF484 0.57 +0.5 206,018 + GFF484 0.62 +1.6 206,019 - GFF484 0.62 +0.1 206,219 - GFF484 0.74 +0.1 206,263 + GFF484 0.76 +0.4 206,386 - GFF484 0.84 +0.4 206,486 - GFF484 0.90 +0.4 206,609 + +0.1 206,709 + GFF485 0.28 -0.4 206,710 - GFF485 0.29 +0.2 206,731 - GFF485 0.40 +0.2 206,745 - GFF485 0.47 +0.1 206,928 - -0.2 206,987 + +0.2 206,988 - +0.3 207,079 + GFF486 0.15 +0.6 207,157 + GFF486 0.20 -0.0 207,158 - GFF486 0.20 +0.8 207,186 - GFF486 0.22 -0.2 207,192 - GFF486 0.22 -0.0 207,283 + GFF486 0.28 +0.8 207,284 - GFF486 0.28 +0.6 207,413 - GFF486 0.37 +0.3 207,511 + GFF486 0.43 +0.3 207,604 + GFF486 0.49 +0.1
Or see this region's nucleotide sequence