Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4800 and GFF4801 are separated by 250 nucleotides GFF4801 and GFF4802 are separated by 1 nucleotides GFF4802 and GFF4803 are separated by 190 nucleotides GFF4803 and GFF4804 are separated by 69 nucleotides GFF4804 and GFF4805 overlap by 10 nucleotides GFF4805 and GFF4806 are separated by 63 nucleotides
GFF4800 - Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4), at 104,174 to 105,082
GFF4800
GFF4801 - Alkaline phosphatase isozyme conversion protein precursor (EC 3.4.11.-), at 105,333 to 106,379
GFF4801
GFF4802 - FIG01045727: hypothetical protein, at 106,381 to 106,554
GFF4802
GFF4803 - hypothetical protein, at 106,745 to 106,891
GFF4803
GFF4804 - hypothetical protein, at 106,961 to 107,092
GFF4804
GFF4805 - FIG01046225: hypothetical protein, at 107,083 to 107,202
GFF4805
GFF4806 - FIG01045727: hypothetical protein, at 107,266 to 107,457
GFF4806
Position (kb)
105
106
107 Strain fitness (log2 ratio)
-1
0
1 at 104.494 kb on - strand, within GFF4800 at 104.572 kb on - strand, within GFF4800 at 104.727 kb on - strand, within GFF4800 at 104.812 kb on + strand, within GFF4800 at 104.813 kb on - strand, within GFF4800 at 104.816 kb on + strand, within GFF4800 at 104.865 kb on + strand, within GFF4800 at 104.865 kb on + strand, within GFF4800 at 104.865 kb on + strand, within GFF4800 at 104.904 kb on - strand, within GFF4800 at 104.982 kb on + strand, within GFF4800 at 104.983 kb on - strand, within GFF4800 at 105.025 kb on + strand at 105.083 kb on - strand at 105.125 kb on + strand at 105.136 kb on - strand at 105.178 kb on + strand at 105.317 kb on + strand at 105.321 kb on + strand at 105.434 kb on + strand at 105.563 kb on + strand, within GFF4801 at 105.575 kb on + strand, within GFF4801 at 105.581 kb on + strand, within GFF4801 at 105.582 kb on - strand, within GFF4801 at 105.689 kb on + strand, within GFF4801 at 105.694 kb on - strand, within GFF4801 at 105.762 kb on - strand, within GFF4801 at 105.814 kb on + strand, within GFF4801 at 105.872 kb on - strand, within GFF4801 at 105.872 kb on - strand, within GFF4801 at 105.974 kb on + strand, within GFF4801 at 106.253 kb on - strand, within GFF4801 at 106.321 kb on + strand at 106.322 kb on - strand at 106.322 kb on - strand at 106.322 kb on - strand at 106.322 kb on - strand at 106.381 kb on - strand at 106.399 kb on + strand, within GFF4802 at 106.555 kb on + strand at 106.555 kb on + strand at 106.555 kb on + strand at 106.675 kb on + strand at 106.675 kb on + strand at 106.677 kb on + strand at 106.678 kb on - strand at 107.157 kb on - strand, within GFF4805 at 107.163 kb on - strand, within GFF4805 at 107.212 kb on + strand at 107.212 kb on + strand at 107.213 kb on - strand at 107.324 kb on + strand, within GFF4806
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 104,494 - GFF4800 0.35 +0.6 104,572 - GFF4800 0.44 +0.5 104,727 - GFF4800 0.61 -0.2 104,812 + GFF4800 0.70 +0.8 104,813 - GFF4800 0.70 +0.5 104,816 + GFF4800 0.71 +0.2 104,865 + GFF4800 0.76 +0.2 104,865 + GFF4800 0.76 +0.1 104,865 + GFF4800 0.76 +0.5 104,904 - GFF4800 0.80 +0.6 104,982 + GFF4800 0.89 +1.3 104,983 - GFF4800 0.89 +0.9 105,025 + +1.6 105,083 - -0.6 105,125 + +0.9 105,136 - -0.9 105,178 + +0.6 105,317 + +0.2 105,321 + +0.2 105,434 + -0.1 105,563 + GFF4801 0.22 -0.5 105,575 + GFF4801 0.23 -1.5 105,581 + GFF4801 0.24 +0.2 105,582 - GFF4801 0.24 +0.0 105,689 + GFF4801 0.34 +1.5 105,694 - GFF4801 0.34 -1.1 105,762 - GFF4801 0.41 -0.1 105,814 + GFF4801 0.46 -1.6 105,872 - GFF4801 0.51 -1.0 105,872 - GFF4801 0.51 -1.2 105,974 + GFF4801 0.61 +0.2 106,253 - GFF4801 0.88 +0.7 106,321 + +0.1 106,322 - +0.2 106,322 - +0.9 106,322 - -0.5 106,322 - +0.3 106,381 - -0.1 106,399 + GFF4802 0.10 +0.6 106,555 + +0.1 106,555 + -0.6 106,555 + +0.3 106,675 + +0.3 106,675 + +0.3 106,677 + -0.6 106,678 - -0.9 107,157 - GFF4805 0.62 -0.1 107,163 - GFF4805 0.67 -0.3 107,212 + +0.1 107,212 + -0.9 107,213 - +0.2 107,324 + GFF4806 0.30 +0.2
Or see this region's nucleotide sequence