Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4714 and GFF4715 are separated by 17 nucleotides GFF4715 and GFF4716 are separated by 2 nucleotides GFF4716 and GFF4717 overlap by 1 nucleotides GFF4717 and GFF4718 are separated by 145 nucleotides
GFF4714 - Formate hydrogenlyase subunit 5, at 23,674 to 25,383
GFF4714
GFF4715 - Formate hydrogenlyase subunit 4, at 25,401 to 26,324
GFF4715
GFF4716 - Formate hydrogenlyase subunit 3, at 26,327 to 28,153
GFF4716
GFF4717 - Formate hydrogenlyase subunit 2, at 28,153 to 28,761
GFF4717
GFF4718 - Formate hydrogenlyase regulatory protein HycA, at 28,907 to 29,368
GFF4718
Position (kb) 26
27
28
29 Strain fitness (log2 ratio) -1
0
1 at 25.356 kb on - strand at 25.398 kb on - strand at 25.832 kb on - strand, within GFF4715 at 25.857 kb on - strand, within GFF4715 at 25.857 kb on - strand, within GFF4715 at 26.020 kb on + strand, within GFF4715 at 26.034 kb on + strand, within GFF4715 at 26.512 kb on + strand, within GFF4716 at 26.602 kb on + strand, within GFF4716 at 26.618 kb on + strand, within GFF4716 at 26.864 kb on - strand, within GFF4716 at 26.864 kb on - strand, within GFF4716 at 26.919 kb on - strand, within GFF4716 at 26.970 kb on + strand, within GFF4716 at 27.067 kb on + strand, within GFF4716 at 27.520 kb on + strand, within GFF4716 at 27.843 kb on - strand, within GFF4716 at 27.908 kb on - strand, within GFF4716 at 28.133 kb on - strand at 28.133 kb on - strand at 28.210 kb on - strand at 28.227 kb on + strand, within GFF4717 at 28.228 kb on - strand, within GFF4717 at 28.494 kb on + strand, within GFF4717 at 28.531 kb on - strand, within GFF4717 at 28.557 kb on + strand, within GFF4717 at 28.828 kb on + strand at 28.908 kb on + strand at 28.977 kb on + strand, within GFF4718 at 29.013 kb on + strand, within GFF4718 at 29.013 kb on + strand, within GFF4718 at 29.014 kb on - strand, within GFF4718
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 25,356 - +0.1 25,398 - +0.3 25,832 - GFF4715 0.47 +0.2 25,857 - GFF4715 0.49 +0.1 25,857 - GFF4715 0.49 +0.4 26,020 + GFF4715 0.67 +1.1 26,034 + GFF4715 0.69 +0.4 26,512 + GFF4716 0.10 +0.6 26,602 + GFF4716 0.15 -0.3 26,618 + GFF4716 0.16 -1.2 26,864 - GFF4716 0.29 +0.8 26,864 - GFF4716 0.29 +0.1 26,919 - GFF4716 0.32 +0.1 26,970 + GFF4716 0.35 -0.1 27,067 + GFF4716 0.41 -0.8 27,520 + GFF4716 0.65 -0.4 27,843 - GFF4716 0.83 -0.1 27,908 - GFF4716 0.87 -0.7 28,133 - +0.2 28,133 - -0.1 28,210 - -1.4 28,227 + GFF4717 0.12 +0.4 28,228 - GFF4717 0.12 +0.1 28,494 + GFF4717 0.56 -0.6 28,531 - GFF4717 0.62 +0.1 28,557 + GFF4717 0.66 -1.4 28,828 + -0.1 28,908 + -0.4 28,977 + GFF4718 0.15 -0.1 29,013 + GFF4718 0.23 +0.1 29,013 + GFF4718 0.23 +0.4 29,014 - GFF4718 0.23 -1.7
Or see this region's nucleotide sequence