Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF467 and GFF468 are separated by 17 nucleotides GFF468 and GFF469 are separated by 5 nucleotides GFF469 and GFF470 are separated by 217 nucleotides
GFF467 - type 1 fimbriae anchoring protein FimD, at 186,435 to 188,963
GFF467
GFF468 - Putative fimbrial protein, at 188,981 to 190,060
GFF468
GFF469 - putative fimbrial protein precursor, at 190,066 to 190,593
GFF469
GFF470 - Phosphoethanolamine transferase specific for the outer Kdo residue of lipopolysaccharide, at 190,811 to 192,502
GFF470
Position (kb) 188
189
190
191 Strain fitness (log2 ratio) -1
0
1
2 at 187.992 kb on + strand, within GFF467 at 188.034 kb on - strand, within GFF467 at 188.085 kb on + strand, within GFF467 at 188.085 kb on + strand, within GFF467 at 188.098 kb on - strand, within GFF467 at 188.140 kb on + strand, within GFF467 at 188.183 kb on + strand, within GFF467 at 188.271 kb on + strand, within GFF467 at 188.272 kb on - strand, within GFF467 at 188.272 kb on - strand, within GFF467 at 188.273 kb on + strand, within GFF467 at 188.374 kb on - strand, within GFF467 at 188.374 kb on - strand, within GFF467 at 188.374 kb on - strand, within GFF467 at 188.374 kb on - strand, within GFF467 at 188.374 kb on - strand, within GFF467 at 188.401 kb on - strand, within GFF467 at 188.438 kb on + strand, within GFF467 at 188.438 kb on + strand, within GFF467 at 188.477 kb on + strand, within GFF467 at 188.563 kb on - strand, within GFF467 at 188.651 kb on - strand, within GFF467 at 188.824 kb on - strand at 188.824 kb on - strand at 188.925 kb on - strand at 188.980 kb on + strand at 189.074 kb on - strand at 189.094 kb on + strand, within GFF468 at 189.094 kb on + strand, within GFF468 at 189.095 kb on - strand, within GFF468 at 189.095 kb on - strand, within GFF468 at 189.111 kb on + strand, within GFF468 at 189.112 kb on - strand, within GFF468 at 189.112 kb on - strand, within GFF468 at 189.115 kb on + strand, within GFF468 at 189.195 kb on - strand, within GFF468 at 189.195 kb on - strand, within GFF468 at 189.223 kb on + strand, within GFF468 at 189.300 kb on - strand, within GFF468 at 189.316 kb on + strand, within GFF468 at 189.319 kb on + strand, within GFF468 at 189.365 kb on + strand, within GFF468 at 189.365 kb on + strand, within GFF468 at 189.366 kb on - strand, within GFF468 at 189.390 kb on + strand, within GFF468 at 189.436 kb on + strand, within GFF468 at 189.450 kb on + strand, within GFF468 at 189.478 kb on - strand, within GFF468 at 189.478 kb on - strand, within GFF468 at 189.514 kb on + strand, within GFF468 at 189.519 kb on + strand, within GFF468 at 189.520 kb on - strand, within GFF468 at 189.535 kb on - strand, within GFF468 at 189.567 kb on + strand, within GFF468 at 189.567 kb on + strand, within GFF468 at 189.577 kb on + strand, within GFF468 at 189.577 kb on + strand, within GFF468 at 189.579 kb on + strand, within GFF468 at 189.579 kb on + strand, within GFF468 at 189.580 kb on - strand, within GFF468 at 189.580 kb on - strand, within GFF468 at 189.595 kb on - strand, within GFF468 at 189.597 kb on - strand, within GFF468 at 189.598 kb on + strand, within GFF468 at 189.598 kb on + strand, within GFF468 at 189.598 kb on + strand, within GFF468 at 189.599 kb on - strand, within GFF468 at 189.599 kb on - strand, within GFF468 at 189.599 kb on - strand, within GFF468 at 190.045 kb on + strand at 190.046 kb on - strand at 190.059 kb on - strand at 190.164 kb on + strand, within GFF469 at 190.174 kb on - strand, within GFF469 at 190.176 kb on + strand, within GFF469 at 190.209 kb on + strand, within GFF469 at 190.209 kb on + strand, within GFF469 at 190.439 kb on - strand, within GFF469 at 190.492 kb on - strand, within GFF469 at 190.508 kb on - strand, within GFF469 at 190.509 kb on + strand, within GFF469 at 190.519 kb on + strand, within GFF469 at 190.520 kb on - strand, within GFF469 at 190.586 kb on - strand at 190.698 kb on + strand at 190.702 kb on + strand at 190.703 kb on - strand at 190.724 kb on + strand at 190.724 kb on + strand at 190.725 kb on - strand at 190.725 kb on - strand at 190.744 kb on - strand at 190.748 kb on - strand at 190.748 kb on - strand at 190.748 kb on - strand at 190.865 kb on + strand at 190.866 kb on - strand at 190.916 kb on + strand at 190.917 kb on - strand at 190.996 kb on - strand, within GFF470
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 187,992 + GFF467 0.62 +0.4 188,034 - GFF467 0.63 +0.2 188,085 + GFF467 0.65 -0.1 188,085 + GFF467 0.65 +0.0 188,098 - GFF467 0.66 +0.5 188,140 + GFF467 0.67 -0.8 188,183 + GFF467 0.69 +0.6 188,271 + GFF467 0.73 -0.2 188,272 - GFF467 0.73 +0.1 188,272 - GFF467 0.73 -1.0 188,273 + GFF467 0.73 -0.1 188,374 - GFF467 0.77 -0.2 188,374 - GFF467 0.77 +0.5 188,374 - GFF467 0.77 +1.8 188,374 - GFF467 0.77 -0.1 188,374 - GFF467 0.77 -0.1 188,401 - GFF467 0.78 -0.1 188,438 + GFF467 0.79 -1.1 188,438 + GFF467 0.79 -0.8 188,477 + GFF467 0.81 -0.3 188,563 - GFF467 0.84 +0.6 188,651 - GFF467 0.88 -0.7 188,824 - +0.0 188,824 - -0.0 188,925 - -0.0 188,980 + +0.0 189,074 - +0.0 189,094 + GFF468 0.10 +0.4 189,094 + GFF468 0.10 -0.7 189,095 - GFF468 0.11 -0.2 189,095 - GFF468 0.11 +0.0 189,111 + GFF468 0.12 -0.6 189,112 - GFF468 0.12 -0.0 189,112 - GFF468 0.12 -0.1 189,115 + GFF468 0.12 +0.3 189,195 - GFF468 0.20 -0.3 189,195 - GFF468 0.20 -0.2 189,223 + GFF468 0.22 +1.1 189,300 - GFF468 0.30 -1.1 189,316 + GFF468 0.31 -0.4 189,319 + GFF468 0.31 +0.6 189,365 + GFF468 0.36 +0.1 189,365 + GFF468 0.36 -1.3 189,366 - GFF468 0.36 +0.2 189,390 + GFF468 0.38 +0.0 189,436 + GFF468 0.42 +0.2 189,450 + GFF468 0.43 -0.3 189,478 - GFF468 0.46 -0.5 189,478 - GFF468 0.46 -0.5 189,514 + GFF468 0.49 +0.3 189,519 + GFF468 0.50 +0.3 189,520 - GFF468 0.50 -0.4 189,535 - GFF468 0.51 -0.0 189,567 + GFF468 0.54 -0.2 189,567 + GFF468 0.54 -1.6 189,577 + GFF468 0.55 +0.6 189,577 + GFF468 0.55 -0.3 189,579 + GFF468 0.55 -0.0 189,579 + GFF468 0.55 -0.0 189,580 - GFF468 0.55 -0.2 189,580 - GFF468 0.55 -0.4 189,595 - GFF468 0.57 -0.2 189,597 - GFF468 0.57 -0.0 189,598 + GFF468 0.57 +0.1 189,598 + GFF468 0.57 +0.1 189,598 + GFF468 0.57 -0.6 189,599 - GFF468 0.57 -0.7 189,599 - GFF468 0.57 -0.1 189,599 - GFF468 0.57 -0.3 190,045 + +0.1 190,046 - +0.2 190,059 - +1.1 190,164 + GFF469 0.19 +0.1 190,174 - GFF469 0.20 +0.1 190,176 + GFF469 0.21 +0.1 190,209 + GFF469 0.27 +0.1 190,209 + GFF469 0.27 +0.1 190,439 - GFF469 0.71 +0.5 190,492 - GFF469 0.81 -0.5 190,508 - GFF469 0.84 -0.6 190,509 + GFF469 0.84 -0.3 190,519 + GFF469 0.86 +0.2 190,520 - GFF469 0.86 -0.1 190,586 - +0.4 190,698 + -0.6 190,702 + -0.2 190,703 - -0.2 190,724 + -0.2 190,724 + +0.3 190,725 - -0.9 190,725 - -0.7 190,744 - -0.1 190,748 - -0.1 190,748 - +0.7 190,748 - +0.3 190,865 + +0.0 190,866 - +0.0 190,916 + -0.9 190,917 - +0.4 190,996 - GFF470 0.11 -1.0
Or see this region's nucleotide sequence