Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4614 and GFF4615 are separated by 171 nucleotides GFF4615 and GFF4616 are separated by 15 nucleotides GFF4616 and GFF4617 overlap by 7 nucleotides GFF4617 and GFF4618 overlap by 11 nucleotides
GFF4614 - hypothetical protein, at 408,307 to 408,420
GFF4614
GFF4615 - FIG137478: Hypothetical protein YbgI, at 408,592 to 409,335
GFF4615
GFF4616 - Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54), at 409,351 to 410,007
GFF4616
GFF4617 - Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54), at 410,001 to 410,933
GFF4617
GFF4618 - Lactam utilization protein LamB, at 410,923 to 411,657
GFF4618
Position (kb) 409
410
411 Strain fitness (log2 ratio) -1
0
1 at 408.532 kb on + strand at 408.566 kb on + strand at 408.578 kb on + strand at 409.126 kb on - strand, within GFF4615 at 409.126 kb on - strand, within GFF4615 at 409.369 kb on + strand at 409.369 kb on + strand at 409.707 kb on - strand, within GFF4616 at 409.724 kb on - strand, within GFF4616 at 409.791 kb on + strand, within GFF4616 at 410.009 kb on + strand at 410.010 kb on - strand at 410.012 kb on + strand at 410.030 kb on + strand at 410.300 kb on + strand, within GFF4617 at 410.330 kb on + strand, within GFF4617 at 410.331 kb on - strand, within GFF4617 at 410.331 kb on - strand, within GFF4617 at 410.383 kb on - strand, within GFF4617 at 410.383 kb on - strand, within GFF4617 at 410.384 kb on + strand, within GFF4617 at 410.384 kb on + strand, within GFF4617 at 410.504 kb on - strand, within GFF4617 at 410.504 kb on - strand, within GFF4617 at 410.713 kb on - strand, within GFF4617 at 410.726 kb on + strand, within GFF4617 at 410.727 kb on - strand, within GFF4617 at 410.795 kb on + strand, within GFF4617 at 410.795 kb on + strand, within GFF4617 at 410.795 kb on + strand, within GFF4617 at 410.796 kb on - strand, within GFF4617 at 410.796 kb on - strand, within GFF4617 at 410.906 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 408,532 + +0.5 408,566 + -1.1 408,578 + -0.4 409,126 - GFF4615 0.72 -0.0 409,126 - GFF4615 0.72 +0.4 409,369 + -0.1 409,369 + +0.1 409,707 - GFF4616 0.54 +0.8 409,724 - GFF4616 0.57 +0.5 409,791 + GFF4616 0.67 +0.4 410,009 + +0.6 410,010 - -0.7 410,012 + -0.3 410,030 + +0.4 410,300 + GFF4617 0.32 +0.3 410,330 + GFF4617 0.35 -0.4 410,331 - GFF4617 0.35 +0.1 410,331 - GFF4617 0.35 +0.4 410,383 - GFF4617 0.41 -0.2 410,383 - GFF4617 0.41 -0.4 410,384 + GFF4617 0.41 +0.0 410,384 + GFF4617 0.41 +0.4 410,504 - GFF4617 0.54 +0.6 410,504 - GFF4617 0.54 -1.1 410,713 - GFF4617 0.76 +0.0 410,726 + GFF4617 0.78 +0.2 410,727 - GFF4617 0.78 +0.4 410,795 + GFF4617 0.85 -0.9 410,795 + GFF4617 0.85 -0.7 410,795 + GFF4617 0.85 +0.2 410,796 - GFF4617 0.85 +0.7 410,796 - GFF4617 0.85 -0.2 410,906 + -0.1
Or see this region's nucleotide sequence