Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4580 and GFF4581 are separated by 66 nucleotides GFF4581 and GFF4659 are separated by 135 nucleotides GFF4659 and GFF4660 are separated by 43 nucleotides GFF4660 and GFF4661 are separated by 46 nucleotides GFF4661 and GFF4662 are separated by 15 nucleotides GFF4662 and GFF4663 are separated by 35 nucleotides GFF4663 and GFF4664 are separated by 35 nucleotides GFF4664 and GFF4665 are separated by 35 nucleotides GFF4665 and GFF4666 are separated by 35 nucleotides
GFF4580 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (EC 1.14.13.-), at 366,269 to 367,444
GFF4580
GFF4581 - Putative inner membrane protein, at 367,511 to 368,086
GFF4581
GFF4659 - tRNA-Gln-CTG, at 368,222 to 368,296
GFF4659
GFF4660 - tRNA-Gln-CTG, at 368,340 to 368,414
GFF4660
GFF4661 - tRNA-Met-CAT, at 368,461 to 368,537
GFF4661
GFF4662 - tRNA-Gln-TTG, at 368,553 to 368,627
GFF4662
GFF4663 - tRNA-Gln-TTG, at 368,663 to 368,737
GFF4663
GFF4664 - tRNA-Gln-TTG, at 368,773 to 368,847
GFF4664
GFF4665 - tRNA-Gln-TTG, at 368,883 to 368,957
GFF4665
GFF4666 - tRNA-Gln-TTG, at 368,993 to 369,067
GFF4666
Position (kb)
367
368
369 Strain fitness (log2 ratio)
-2
-1
0
1 at 367.424 kb on - strand at 367.428 kb on - strand at 367.495 kb on + strand at 367.513 kb on - strand at 367.526 kb on + strand at 367.595 kb on + strand, within GFF4581 at 367.595 kb on + strand, within GFF4581 at 367.614 kb on - strand, within GFF4581 at 367.621 kb on + strand, within GFF4581 at 367.621 kb on + strand, within GFF4581 at 367.622 kb on - strand, within GFF4581 at 367.736 kb on - strand, within GFF4581 at 367.785 kb on + strand, within GFF4581 at 367.785 kb on + strand, within GFF4581 at 367.869 kb on + strand, within GFF4581 at 367.869 kb on + strand, within GFF4581 at 367.870 kb on - strand, within GFF4581 at 367.925 kb on + strand, within GFF4581 at 367.926 kb on - strand, within GFF4581 at 367.926 kb on - strand, within GFF4581 at 367.927 kb on + strand, within GFF4581 at 367.927 kb on + strand, within GFF4581 at 368.045 kb on + strand at 368.064 kb on + strand at 368.132 kb on + strand at 368.174 kb on + strand at 368.460 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 367,424 - -1.2 367,428 - -1.4 367,495 + -0.7 367,513 - -0.4 367,526 + +0.4 367,595 + GFF4581 0.15 -0.7 367,595 + GFF4581 0.15 +0.3 367,614 - GFF4581 0.18 -1.7 367,621 + GFF4581 0.19 -0.2 367,621 + GFF4581 0.19 +0.2 367,622 - GFF4581 0.19 -0.1 367,736 - GFF4581 0.39 -1.9 367,785 + GFF4581 0.48 +0.2 367,785 + GFF4581 0.48 -1.7 367,869 + GFF4581 0.62 +0.0 367,869 + GFF4581 0.62 -0.3 367,870 - GFF4581 0.62 -0.7 367,925 + GFF4581 0.72 +0.1 367,926 - GFF4581 0.72 -0.3 367,926 - GFF4581 0.72 -0.1 367,927 + GFF4581 0.72 -0.3 367,927 + GFF4581 0.72 +0.3 368,045 + -0.1 368,064 + -1.0 368,132 + -0.1 368,174 + -0.5 368,460 - +0.6
Or see this region's nucleotide sequence