Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF454

Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF453 and GFF454 are separated by 173 nucleotidesGFF454 and GFF455 are separated by 288 nucleotidesGFF455 and GFF456 are separated by 157 nucleotides GFF453 - FIG00923183: possible membrane protein YebY, at 175,714 to 176,253 GFF453 GFF454 - Cold shock protein CspA, at 176,427 to 176,639 GFF454 GFF455 - FIG00638451: hypothetical protein, at 176,928 to 177,218 GFF455 GFF456 - hypothetical protein, at 177,376 to 177,507 GFF456 Position (kb) 176 177Strain fitness (log2 ratio) -1 0 1at 175.540 kb on + strandat 175.799 kb on - strand, within GFF453at 176.031 kb on + strand, within GFF453at 176.053 kb on + strand, within GFF453at 176.233 kb on + strandat 176.253 kb on + strandat 176.286 kb on + strandat 176.336 kb on - strandat 176.559 kb on - strand, within GFF454at 176.577 kb on - strand, within GFF454at 176.580 kb on - strand, within GFF454at 176.614 kb on - strand, within GFF454at 176.640 kb on - strandat 176.640 kb on - strandat 176.645 kb on - strandat 176.648 kb on - strandat 176.768 kb on - strandat 176.848 kb on + strandat 176.894 kb on - strandat 176.949 kb on + strandat 176.950 kb on - strandat 177.097 kb on - strand, within GFF455at 177.209 kb on + strandat 177.391 kb on - strand, within GFF456at 177.434 kb on + strand, within GFF456at 177.529 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI
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175,540 + -0.1
175,799 - GFF453 0.16 -0.1
176,031 + GFF453 0.59 +1.1
176,053 + GFF453 0.63 +0.2
176,233 + -0.2
176,253 + -0.1
176,286 + -0.5
176,336 - -0.4
176,559 - GFF454 0.62 +1.4
176,577 - GFF454 0.70 +0.3
176,580 - GFF454 0.72 +0.5
176,614 - GFF454 0.88 -0.2
176,640 - +0.2
176,640 - -0.0
176,645 - +0.2
176,648 - +0.1
176,768 - +0.5
176,848 + +0.1
176,894 - +0.3
176,949 + -0.4
176,950 - +0.1
177,097 - GFF455 0.58 +0.4
177,209 + -0.1
177,391 - GFF456 0.11 -0.2
177,434 + GFF456 0.44 +0.2
177,529 + -0.7

Or see this region's nucleotide sequence