Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4478 and GFF4479 are separated by 2 nucleotides GFF4479 and GFF4480 are separated by 14 nucleotides GFF4480 and GFF4481 overlap by 23 nucleotides GFF4481 and GFF4482 are separated by 199 nucleotides
GFF4478 - PTS system, mannose-specific IID component (EC 2.7.1.69), at 264,530 to 265,384
GFF4478
GFF4479 - PTS system, mannose-specific IIC component (EC 2.7.1.69), at 265,387 to 266,130
GFF4479
GFF4480 - PTS system, mannose-specific IIB component (EC 2.7.1.69), at 266,145 to 266,615
GFF4480
GFF4481 - PTS system, mannose-specific IIA component (EC 2.7.1.69), at 266,593 to 267,042
GFF4481
GFF4482 - Oxygen-insensitive NAD(P)H nitroreductase (EC 1.-.-.-) / Dihydropteridine reductase (EC 1.5.1.34), at 267,242 to 267,895
GFF4482
Position (kb) 266
267 Strain fitness (log2 ratio) -1
0
1 at 265.258 kb on - strand, within GFF4478 at 265.280 kb on - strand, within GFF4478 at 265.480 kb on - strand, within GFF4479 at 265.511 kb on + strand, within GFF4479 at 265.511 kb on + strand, within GFF4479 at 265.512 kb on - strand, within GFF4479 at 265.512 kb on - strand, within GFF4479 at 265.530 kb on + strand, within GFF4479 at 265.531 kb on - strand, within GFF4479 at 265.603 kb on - strand, within GFF4479 at 265.603 kb on - strand, within GFF4479 at 265.743 kb on + strand, within GFF4479 at 265.827 kb on + strand, within GFF4479 at 265.834 kb on - strand, within GFF4479 at 265.879 kb on - strand, within GFF4479 at 266.130 kb on + strand at 266.154 kb on - strand at 266.159 kb on + strand at 266.257 kb on + strand, within GFF4480 at 266.258 kb on - strand, within GFF4480 at 266.260 kb on - strand, within GFF4480 at 266.363 kb on + strand, within GFF4480 at 266.478 kb on + strand, within GFF4480 at 266.479 kb on - strand, within GFF4480 at 266.487 kb on - strand, within GFF4480 at 266.604 kb on + strand at 266.630 kb on - strand at 266.653 kb on + strand, within GFF4481 at 266.700 kb on - strand, within GFF4481 at 266.936 kb on + strand, within GFF4481 at 266.991 kb on + strand, within GFF4481 at 267.030 kb on - strand at 267.030 kb on - strand at 267.146 kb on + strand at 267.349 kb on - strand, within GFF4482 at 267.466 kb on - strand, within GFF4482 at 267.556 kb on + strand, within GFF4482
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 265,258 - GFF4478 0.85 +0.1 265,280 - GFF4478 0.88 +0.1 265,480 - GFF4479 0.12 +0.2 265,511 + GFF4479 0.17 -0.6 265,511 + GFF4479 0.17 -0.3 265,512 - GFF4479 0.17 +0.2 265,512 - GFF4479 0.17 +0.5 265,530 + GFF4479 0.19 -0.0 265,531 - GFF4479 0.19 +0.2 265,603 - GFF4479 0.29 +1.7 265,603 - GFF4479 0.29 +0.3 265,743 + GFF4479 0.48 -0.1 265,827 + GFF4479 0.59 -0.7 265,834 - GFF4479 0.60 -0.5 265,879 - GFF4479 0.66 +0.3 266,130 + -0.2 266,154 - -0.3 266,159 + +0.4 266,257 + GFF4480 0.24 -0.8 266,258 - GFF4480 0.24 -0.6 266,260 - GFF4480 0.24 -0.8 266,363 + GFF4480 0.46 -0.7 266,478 + GFF4480 0.71 -1.1 266,479 - GFF4480 0.71 -0.3 266,487 - GFF4480 0.73 +0.1 266,604 + +0.7 266,630 - -0.8 266,653 + GFF4481 0.13 -0.9 266,700 - GFF4481 0.24 -0.1 266,936 + GFF4481 0.76 +0.3 266,991 + GFF4481 0.88 +0.5 267,030 - +0.4 267,030 - +0.0 267,146 + -0.1 267,349 - GFF4482 0.16 +0.8 267,466 - GFF4482 0.34 -1.1 267,556 + GFF4482 0.48 -0.8
Or see this region's nucleotide sequence