Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4474 and GFF4475 are separated by 253 nucleotides GFF4475 and GFF4476 are separated by 127 nucleotides
GFF4474 - Outer membrane esterase, at 257,407 to 259,377
GFF4474
GFF4475 - NtrC family Transcriptional regulator, ATPase domain, at 259,631 to 262,273
GFF4475
GFF4476 - Putative phosphosugar isomerase, at 262,401 to 263,387
GFF4476
Position (kb) 259
260
261
262
263 Strain fitness (log2 ratio) -1
0
1 at 258.739 kb on + strand, within GFF4474 at 258.740 kb on - strand, within GFF4474 at 258.860 kb on + strand, within GFF4474 at 258.985 kb on + strand, within GFF4474 at 259.038 kb on + strand, within GFF4474 at 259.039 kb on - strand, within GFF4474 at 259.039 kb on - strand, within GFF4474 at 259.141 kb on - strand, within GFF4474 at 259.167 kb on + strand, within GFF4474 at 259.167 kb on + strand, within GFF4474 at 259.167 kb on + strand, within GFF4474 at 259.171 kb on + strand, within GFF4474 at 259.171 kb on + strand, within GFF4474 at 259.171 kb on + strand, within GFF4474 at 259.172 kb on - strand, within GFF4474 at 259.191 kb on + strand at 259.350 kb on + strand at 259.351 kb on - strand at 259.672 kb on + strand at 259.673 kb on - strand at 259.684 kb on + strand at 259.892 kb on - strand at 259.892 kb on - strand at 259.908 kb on - strand, within GFF4475 at 260.060 kb on - strand, within GFF4475 at 260.284 kb on - strand, within GFF4475 at 260.339 kb on - strand, within GFF4475 at 260.394 kb on - strand, within GFF4475 at 260.596 kb on - strand, within GFF4475 at 260.822 kb on - strand, within GFF4475 at 260.827 kb on - strand, within GFF4475 at 260.828 kb on + strand, within GFF4475 at 260.849 kb on - strand, within GFF4475 at 260.924 kb on + strand, within GFF4475 at 260.970 kb on - strand, within GFF4475 at 261.105 kb on - strand, within GFF4475 at 261.189 kb on + strand, within GFF4475 at 261.346 kb on + strand, within GFF4475 at 261.847 kb on - strand, within GFF4475 at 261.858 kb on + strand, within GFF4475 at 262.280 kb on + strand at 262.400 kb on - strand at 262.473 kb on - strand at 262.652 kb on + strand, within GFF4476 at 262.652 kb on + strand, within GFF4476 at 262.711 kb on - strand, within GFF4476 at 262.711 kb on - strand, within GFF4476 at 262.749 kb on - strand, within GFF4476 at 262.754 kb on + strand, within GFF4476 at 262.754 kb on + strand, within GFF4476 at 262.754 kb on + strand, within GFF4476 at 262.754 kb on + strand, within GFF4476 at 262.801 kb on + strand, within GFF4476 at 262.802 kb on - strand, within GFF4476 at 262.854 kb on + strand, within GFF4476 at 262.928 kb on - strand, within GFF4476 at 263.027 kb on - strand, within GFF4476 at 263.038 kb on + strand, within GFF4476 at 263.217 kb on - strand, within GFF4476
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 258,739 + GFF4474 0.68 -0.0 258,740 - GFF4474 0.68 -0.4 258,860 + GFF4474 0.74 +0.8 258,985 + GFF4474 0.80 +0.1 259,038 + GFF4474 0.83 +0.2 259,039 - GFF4474 0.83 +0.3 259,039 - GFF4474 0.83 -0.5 259,141 - GFF4474 0.88 -0.1 259,167 + GFF4474 0.89 +0.3 259,167 + GFF4474 0.89 +0.3 259,167 + GFF4474 0.89 -0.1 259,171 + GFF4474 0.89 +0.5 259,171 + GFF4474 0.89 -0.9 259,171 + GFF4474 0.89 +0.5 259,172 - GFF4474 0.90 -0.0 259,191 + -1.1 259,350 + -1.6 259,351 - +0.3 259,672 + +0.3 259,673 - +0.1 259,684 + +0.6 259,892 - +0.9 259,892 - -0.6 259,908 - GFF4475 0.10 -0.3 260,060 - GFF4475 0.16 -0.1 260,284 - GFF4475 0.25 -1.1 260,339 - GFF4475 0.27 -0.5 260,394 - GFF4475 0.29 -0.3 260,596 - GFF4475 0.37 +1.7 260,822 - GFF4475 0.45 -0.7 260,827 - GFF4475 0.45 +0.2 260,828 + GFF4475 0.45 -0.3 260,849 - GFF4475 0.46 -0.9 260,924 + GFF4475 0.49 +0.2 260,970 - GFF4475 0.51 +0.3 261,105 - GFF4475 0.56 -0.7 261,189 + GFF4475 0.59 -0.7 261,346 + GFF4475 0.65 +0.3 261,847 - GFF4475 0.84 -0.2 261,858 + GFF4475 0.84 +0.3 262,280 + +0.3 262,400 - +0.6 262,473 - +0.2 262,652 + GFF4476 0.25 +0.4 262,652 + GFF4476 0.25 -0.8 262,711 - GFF4476 0.31 -0.1 262,711 - GFF4476 0.31 +0.4 262,749 - GFF4476 0.35 -0.3 262,754 + GFF4476 0.36 -0.7 262,754 + GFF4476 0.36 +0.1 262,754 + GFF4476 0.36 +0.3 262,754 + GFF4476 0.36 -1.1 262,801 + GFF4476 0.41 +0.2 262,802 - GFF4476 0.41 +0.8 262,854 + GFF4476 0.46 -0.1 262,928 - GFF4476 0.53 -0.6 263,027 - GFF4476 0.63 -0.5 263,038 + GFF4476 0.65 +0.2 263,217 - GFF4476 0.83 +0.0
Or see this region's nucleotide sequence