Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4249 and GFF4250 are separated by 77 nucleotides GFF4250 and GFF4251 are separated by 292 nucleotides GFF4251 and GFF4252 are separated by 188 nucleotides
GFF4249 - Cu(I)-responsive transcriptional regulator, at 31,072 to 31,536
GFF4249
GFF4250 - Putative copper chaperone, at 31,614 to 31,808
GFF4250
GFF4251 - Putative metabolite transport protein, at 32,101 to 33,354
GFF4251
GFF4252 - Type III restriction-modification system methylation subunit (EC 2.1.1.72), at 33,543 to 35,501
GFF4252
Position (kb)
32
33
34 Strain fitness (log2 ratio)
-1
0
1 at 31.204 kb on - strand, within GFF4249 at 31.268 kb on + strand, within GFF4249 at 31.268 kb on + strand, within GFF4249 at 31.534 kb on + strand at 31.534 kb on + strand at 31.548 kb on - strand at 31.692 kb on - strand, within GFF4250 at 31.806 kb on + strand at 31.807 kb on - strand at 31.976 kb on - strand at 31.996 kb on + strand at 32.012 kb on - strand at 32.096 kb on + strand at 32.096 kb on + strand at 32.100 kb on + strand at 32.100 kb on + strand at 32.101 kb on - strand at 32.101 kb on - strand at 32.101 kb on - strand at 32.101 kb on - strand at 32.104 kb on + strand at 32.105 kb on - strand at 32.204 kb on + strand at 32.586 kb on + strand, within GFF4251 at 32.592 kb on - strand, within GFF4251 at 32.644 kb on + strand, within GFF4251 at 32.646 kb on + strand, within GFF4251 at 32.646 kb on + strand, within GFF4251 at 32.656 kb on - strand, within GFF4251 at 32.752 kb on + strand, within GFF4251 at 32.752 kb on + strand, within GFF4251 at 32.753 kb on - strand, within GFF4251 at 32.778 kb on + strand, within GFF4251 at 32.814 kb on + strand, within GFF4251 at 32.929 kb on - strand, within GFF4251 at 33.045 kb on + strand, within GFF4251 at 33.046 kb on - strand, within GFF4251 at 33.208 kb on + strand, within GFF4251 at 33.353 kb on - strand at 33.374 kb on + strand at 33.374 kb on + strand at 33.646 kb on - strand at 33.881 kb on + strand, within GFF4252 at 33.932 kb on + strand, within GFF4252 at 33.954 kb on + strand, within GFF4252 at 33.999 kb on + strand, within GFF4252 at 34.023 kb on - strand, within GFF4252 at 34.023 kb on - strand, within GFF4252 at 34.023 kb on - strand, within GFF4252 at 34.041 kb on - strand, within GFF4252 at 34.107 kb on + strand, within GFF4252 at 34.161 kb on - strand, within GFF4252 at 34.194 kb on - strand, within GFF4252 at 34.271 kb on + strand, within GFF4252 at 34.343 kb on - strand, within GFF4252
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 31,204 - GFF4249 0.28 -0.3 31,268 + GFF4249 0.42 +0.6 31,268 + GFF4249 0.42 +0.1 31,534 + -0.0 31,534 + -0.2 31,548 - +0.7 31,692 - GFF4250 0.40 -1.0 31,806 + +0.1 31,807 - -0.4 31,976 - -0.2 31,996 + +0.4 32,012 - +0.1 32,096 + -0.3 32,096 + +0.1 32,100 + -0.4 32,100 + +0.1 32,101 - -0.0 32,101 - -0.4 32,101 - +0.2 32,101 - +0.1 32,104 + +0.6 32,105 - -0.1 32,204 + +0.2 32,586 + GFF4251 0.39 -0.2 32,592 - GFF4251 0.39 -1.0 32,644 + GFF4251 0.43 +0.4 32,646 + GFF4251 0.43 -0.0 32,646 + GFF4251 0.43 -0.8 32,656 - GFF4251 0.44 +0.0 32,752 + GFF4251 0.52 +0.4 32,752 + GFF4251 0.52 +0.0 32,753 - GFF4251 0.52 -0.2 32,778 + GFF4251 0.54 -0.8 32,814 + GFF4251 0.57 -0.3 32,929 - GFF4251 0.66 -0.7 33,045 + GFF4251 0.75 -0.8 33,046 - GFF4251 0.75 -0.7 33,208 + GFF4251 0.88 -0.4 33,353 - -0.6 33,374 + -0.3 33,374 + +1.1 33,646 - +0.5 33,881 + GFF4252 0.17 +0.2 33,932 + GFF4252 0.20 -0.9 33,954 + GFF4252 0.21 +0.0 33,999 + GFF4252 0.23 +0.4 34,023 - GFF4252 0.25 -1.0 34,023 - GFF4252 0.25 -0.4 34,023 - GFF4252 0.25 -0.5 34,041 - GFF4252 0.25 -0.6 34,107 + GFF4252 0.29 +0.0 34,161 - GFF4252 0.32 -0.0 34,194 - GFF4252 0.33 +0.1 34,271 + GFF4252 0.37 -0.3 34,343 - GFF4252 0.41 +0.4
Or see this region's nucleotide sequence