Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4196 and GFF4197 are separated by 92 nucleotides GFF4197 and GFF4198 are separated by 363 nucleotides GFF4198 and GFF4199 overlap by 1 nucleotides GFF4199 and GFF4200 overlap by 8 nucleotides
GFF4196 - NADH pyrophosphatase (EC 3.6.1.22), at 10,481 to 11,254
GFF4196
GFF4197 - Regulator of sigma D, at 11,347 to 11,835
GFF4197
GFF4198 - Thiamin biosynthesis protein ThiC, at 12,199 to 14,094
GFF4198
GFF4199 - Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3), at 14,094 to 14,729
GFF4199
GFF4200 - Sulfur carrier protein adenylyltransferase ThiF, at 14,722 to 15,480
GFF4200
Position (kb)
12
13
14
15 Strain fitness (log2 ratio)
-1
0
1 at 11.243 kb on + strand at 11.254 kb on + strand at 11.254 kb on + strand at 11.254 kb on + strand at 11.254 kb on + strand at 11.255 kb on - strand at 11.255 kb on - strand at 11.255 kb on - strand at 11.325 kb on + strand at 11.329 kb on + strand at 11.330 kb on - strand at 11.330 kb on - strand at 11.352 kb on - strand at 11.420 kb on + strand, within GFF4197 at 11.424 kb on + strand, within GFF4197 at 11.424 kb on + strand, within GFF4197 at 11.425 kb on - strand, within GFF4197 at 11.446 kb on + strand, within GFF4197 at 11.447 kb on - strand, within GFF4197 at 11.464 kb on - strand, within GFF4197 at 11.483 kb on - strand, within GFF4197 at 11.559 kb on + strand, within GFF4197 at 11.560 kb on - strand, within GFF4197 at 11.564 kb on - strand, within GFF4197 at 11.571 kb on + strand, within GFF4197 at 11.643 kb on + strand, within GFF4197 at 11.700 kb on + strand, within GFF4197 at 11.781 kb on + strand, within GFF4197 at 11.782 kb on - strand, within GFF4197 at 11.782 kb on - strand, within GFF4197 at 11.849 kb on - strand at 11.856 kb on - strand at 11.861 kb on - strand at 11.906 kb on - strand at 11.971 kb on + strand at 11.972 kb on - strand at 11.972 kb on - strand at 11.972 kb on - strand at 11.972 kb on - strand at 11.975 kb on + strand at 11.976 kb on - strand at 11.976 kb on - strand at 12.079 kb on + strand at 12.082 kb on + strand at 12.083 kb on - strand at 12.104 kb on - strand at 12.198 kb on + strand at 12.198 kb on + strand at 12.205 kb on - strand at 12.299 kb on - strand at 12.322 kb on - strand at 12.376 kb on - strand at 12.416 kb on + strand, within GFF4198 at 12.419 kb on - strand, within GFF4198 at 12.511 kb on - strand, within GFF4198 at 12.527 kb on + strand, within GFF4198 at 12.547 kb on + strand, within GFF4198 at 12.548 kb on - strand, within GFF4198 at 12.549 kb on + strand, within GFF4198 at 12.648 kb on - strand, within GFF4198 at 13.021 kb on + strand, within GFF4198 at 13.022 kb on - strand, within GFF4198 at 13.024 kb on - strand, within GFF4198 at 13.053 kb on + strand, within GFF4198 at 13.129 kb on - strand, within GFF4198 at 13.155 kb on - strand, within GFF4198 at 13.216 kb on + strand, within GFF4198 at 13.440 kb on + strand, within GFF4198 at 13.440 kb on + strand, within GFF4198 at 13.521 kb on + strand, within GFF4198 at 13.521 kb on + strand, within GFF4198 at 13.576 kb on + strand, within GFF4198 at 13.682 kb on - strand, within GFF4198 at 14.014 kb on - strand at 14.097 kb on + strand at 14.097 kb on + strand at 14.098 kb on - strand at 14.098 kb on - strand at 14.321 kb on + strand, within GFF4199 at 14.321 kb on + strand, within GFF4199 at 14.322 kb on - strand, within GFF4199 at 14.430 kb on - strand, within GFF4199 at 14.466 kb on + strand, within GFF4199 at 14.467 kb on - strand, within GFF4199 at 14.467 kb on - strand, within GFF4199 at 14.467 kb on - strand, within GFF4199 at 14.549 kb on + strand, within GFF4199 at 14.660 kb on + strand, within GFF4199 at 14.813 kb on + strand, within GFF4200 at 14.813 kb on + strand, within GFF4200 at 14.814 kb on - strand, within GFF4200
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 11,243 + -0.2 11,254 + +0.2 11,254 + +0.3 11,254 + -0.6 11,254 + -0.1 11,255 - +0.2 11,255 - +0.7 11,255 - +0.1 11,325 + -0.5 11,329 + +0.1 11,330 - +0.6 11,330 - +0.3 11,352 - +0.4 11,420 + GFF4197 0.15 +0.3 11,424 + GFF4197 0.16 -0.6 11,424 + GFF4197 0.16 +0.4 11,425 - GFF4197 0.16 -0.2 11,446 + GFF4197 0.20 +0.2 11,447 - GFF4197 0.20 -0.1 11,464 - GFF4197 0.24 -0.3 11,483 - GFF4197 0.28 +0.6 11,559 + GFF4197 0.43 +0.4 11,560 - GFF4197 0.44 +0.4 11,564 - GFF4197 0.44 +0.8 11,571 + GFF4197 0.46 +1.5 11,643 + GFF4197 0.61 -0.1 11,700 + GFF4197 0.72 +0.6 11,781 + GFF4197 0.89 +0.2 11,782 - GFF4197 0.89 +0.1 11,782 - GFF4197 0.89 -0.3 11,849 - +0.2 11,856 - -0.0 11,861 - -0.4 11,906 - -0.5 11,971 + +0.1 11,972 - -0.8 11,972 - -1.0 11,972 - +0.2 11,972 - -1.2 11,975 + +0.2 11,976 - -0.4 11,976 - -0.2 12,079 + +0.4 12,082 + +0.1 12,083 - -1.0 12,104 - +0.1 12,198 + +0.5 12,198 + +0.9 12,205 - +0.3 12,299 - +0.2 12,322 - -0.4 12,376 - -1.0 12,416 + GFF4198 0.11 -0.5 12,419 - GFF4198 0.12 -0.4 12,511 - GFF4198 0.16 -1.0 12,527 + GFF4198 0.17 +0.3 12,547 + GFF4198 0.18 -0.0 12,548 - GFF4198 0.18 -0.7 12,549 + GFF4198 0.18 +0.0 12,648 - GFF4198 0.24 +0.0 13,021 + GFF4198 0.43 -0.1 13,022 - GFF4198 0.43 -0.3 13,024 - GFF4198 0.44 +0.4 13,053 + GFF4198 0.45 +0.3 13,129 - GFF4198 0.49 +0.0 13,155 - GFF4198 0.50 -0.7 13,216 + GFF4198 0.54 -0.4 13,440 + GFF4198 0.65 +0.7 13,440 + GFF4198 0.65 -0.2 13,521 + GFF4198 0.70 -0.0 13,521 + GFF4198 0.70 -0.9 13,576 + GFF4198 0.73 -0.2 13,682 - GFF4198 0.78 +0.1 14,014 - -0.6 14,097 + -0.1 14,097 + -0.5 14,098 - +0.1 14,098 - -0.8 14,321 + GFF4199 0.36 +0.1 14,321 + GFF4199 0.36 +0.4 14,322 - GFF4199 0.36 -0.3 14,430 - GFF4199 0.53 +0.4 14,466 + GFF4199 0.58 +0.1 14,467 - GFF4199 0.59 -0.1 14,467 - GFF4199 0.59 -0.6 14,467 - GFF4199 0.59 -0.3 14,549 + GFF4199 0.72 -0.4 14,660 + GFF4199 0.89 +0.9 14,813 + GFF4200 0.12 -0.3 14,813 + GFF4200 0.12 +0.2 14,814 - GFF4200 0.12 +0.1
Or see this region's nucleotide sequence