Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF418 and GFF419 are separated by 475 nucleotides GFF419 and GFF420 are separated by 98 nucleotides
GFF418 - PTS system, mannitol-specific IIC component (EC 2.7.1.69) / PTS system, mannitol-specific IIB component (EC 2.7.1.69) / PTS system, mannitol-specific IIA component, at 135,895 to 137,811
GFF418
GFF419 - Uncharacterized GST-like protein yibF, at 138,287 to 138,895
GFF419
GFF420 - L-seryl-tRNA(Sec) selenium transferase (EC 2.9.1.1), at 138,994 to 140,385
GFF420
Position (kb)
138
139 Strain fitness (log2 ratio)
-2
-1
0
1 at 137.316 kb on + strand, within GFF418 at 137.317 kb on - strand, within GFF418 at 137.560 kb on - strand, within GFF418 at 137.613 kb on + strand, within GFF418 at 137.640 kb on - strand at 137.700 kb on + strand at 137.715 kb on + strand at 137.742 kb on + strand at 137.745 kb on + strand at 137.757 kb on + strand at 137.758 kb on - strand at 137.827 kb on + strand at 137.827 kb on + strand at 137.827 kb on + strand at 137.870 kb on + strand at 137.901 kb on - strand at 137.901 kb on - strand at 137.905 kb on - strand at 137.954 kb on + strand at 137.973 kb on - strand at 137.994 kb on - strand at 137.994 kb on - strand at 138.006 kb on - strand at 138.251 kb on - strand at 138.251 kb on - strand at 138.263 kb on - strand at 138.301 kb on + strand at 138.356 kb on - strand, within GFF419 at 138.358 kb on + strand, within GFF419 at 138.358 kb on + strand, within GFF419 at 138.359 kb on - strand, within GFF419 at 138.386 kb on + strand, within GFF419 at 138.413 kb on + strand, within GFF419 at 138.462 kb on - strand, within GFF419 at 138.493 kb on + strand, within GFF419 at 138.493 kb on + strand, within GFF419 at 138.586 kb on + strand, within GFF419 at 138.587 kb on - strand, within GFF419 at 138.691 kb on + strand, within GFF419 at 138.692 kb on - strand, within GFF419 at 138.903 kb on + strand at 138.904 kb on - strand at 138.904 kb on - strand at 138.928 kb on + strand at 138.928 kb on + strand at 138.929 kb on - strand at 138.940 kb on + strand at 139.012 kb on - strand at 139.036 kb on - strand at 139.446 kb on + strand, within GFF420 at 139.592 kb on + strand, within GFF420 at 139.672 kb on - strand, within GFF420 at 139.678 kb on - strand, within GFF420 at 139.749 kb on + strand, within GFF420 at 139.793 kb on + strand, within GFF420 at 139.794 kb on - strand, within GFF420 at 139.801 kb on + strand, within GFF420 at 139.876 kb on - strand, within GFF420
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 137,316 + GFF418 0.74 -1.8 137,317 - GFF418 0.74 -1.0 137,560 - GFF418 0.87 +0.4 137,613 + GFF418 0.90 -0.6 137,640 - +0.3 137,700 + +0.1 137,715 + -0.8 137,742 + +0.1 137,745 + -0.2 137,757 + +0.4 137,758 - +0.5 137,827 + +0.1 137,827 + -0.1 137,827 + +0.2 137,870 + -0.0 137,901 - -0.0 137,901 - -0.1 137,905 - +0.2 137,954 + +0.1 137,973 - +0.7 137,994 - -0.2 137,994 - -0.0 138,006 - -0.2 138,251 - +0.0 138,251 - +0.3 138,263 - -0.4 138,301 + -0.2 138,356 - GFF419 0.11 -0.4 138,358 + GFF419 0.12 -0.1 138,358 + GFF419 0.12 -0.7 138,359 - GFF419 0.12 +0.1 138,386 + GFF419 0.16 -0.4 138,413 + GFF419 0.21 +0.3 138,462 - GFF419 0.29 +0.0 138,493 + GFF419 0.34 -0.9 138,493 + GFF419 0.34 -1.1 138,586 + GFF419 0.49 -1.3 138,587 - GFF419 0.49 +0.0 138,691 + GFF419 0.66 -0.7 138,692 - GFF419 0.67 -1.0 138,903 + -1.1 138,904 - +0.1 138,904 - -0.1 138,928 + -0.4 138,928 + +0.4 138,929 - +0.2 138,940 + -0.6 139,012 - -0.1 139,036 - +0.7 139,446 + GFF420 0.32 +0.5 139,592 + GFF420 0.43 -0.2 139,672 - GFF420 0.49 -0.9 139,678 - GFF420 0.49 +0.8 139,749 + GFF420 0.54 +0.3 139,793 + GFF420 0.57 +0.4 139,794 - GFF420 0.57 +0.5 139,801 + GFF420 0.58 +0.3 139,876 - GFF420 0.63 +0.3
Or see this region's nucleotide sequence