Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF415 and GFF416 are separated by 9 nucleotides GFF416 and GFF417 overlap by 4 nucleotides GFF417 and GFF418 are separated by 220 nucleotides
GFF415 - FIG074102: hypothetical protein, at 133,720 to 133,929
GFF415
GFF416 - Mannitol operon repressor, at 133,939 to 134,529
GFF416
GFF417 - Mannitol-1-phosphate 5-dehydrogenase (EC 1.1.1.17), at 134,526 to 135,674
GFF417
GFF418 - PTS system, mannitol-specific IIC component (EC 2.7.1.69) / PTS system, mannitol-specific IIB component (EC 2.7.1.69) / PTS system, mannitol-specific IIA component, at 135,895 to 137,811
GFF418
Position (kb) 134
135
136 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 133.674 kb on - strand at 133.716 kb on + strand at 133.716 kb on + strand at 133.828 kb on + strand, within GFF415 at 133.828 kb on + strand, within GFF415 at 134.045 kb on + strand, within GFF416 at 134.148 kb on - strand, within GFF416 at 134.148 kb on - strand, within GFF416 at 134.208 kb on - strand, within GFF416 at 134.237 kb on + strand, within GFF416 at 134.242 kb on - strand, within GFF416 at 134.242 kb on - strand, within GFF416 at 134.242 kb on - strand, within GFF416 at 134.255 kb on - strand, within GFF416 at 134.306 kb on + strand, within GFF416 at 134.319 kb on - strand, within GFF416 at 134.464 kb on + strand, within GFF416 at 134.475 kb on + strand at 134.476 kb on - strand at 134.670 kb on - strand, within GFF417 at 134.710 kb on - strand, within GFF417 at 134.710 kb on - strand, within GFF417 at 134.725 kb on + strand, within GFF417 at 134.796 kb on + strand, within GFF417 at 134.857 kb on + strand, within GFF417 at 134.858 kb on - strand, within GFF417 at 135.011 kb on + strand, within GFF417 at 135.011 kb on + strand, within GFF417 at 135.011 kb on + strand, within GFF417 at 135.012 kb on - strand, within GFF417 at 135.041 kb on + strand, within GFF417 at 135.041 kb on - strand, within GFF417 at 135.108 kb on - strand, within GFF417 at 135.108 kb on - strand, within GFF417 at 135.197 kb on + strand, within GFF417 at 135.326 kb on + strand, within GFF417 at 135.327 kb on - strand, within GFF417 at 135.393 kb on - strand, within GFF417 at 135.430 kb on - strand, within GFF417 at 135.430 kb on - strand, within GFF417 at 135.430 kb on - strand, within GFF417 at 135.542 kb on + strand, within GFF417 at 135.543 kb on - strand, within GFF417 at 135.543 kb on - strand, within GFF417 at 135.579 kb on + strand at 135.737 kb on - strand at 135.737 kb on - strand at 135.820 kb on - strand at 135.823 kb on + strand at 135.823 kb on + strand at 135.824 kb on - strand at 135.824 kb on - strand at 135.827 kb on + strand at 135.827 kb on + strand at 135.828 kb on - strand at 135.828 kb on - strand at 135.828 kb on - strand at 135.889 kb on + strand at 135.890 kb on - strand at 136.023 kb on + strand at 136.024 kb on - strand at 136.082 kb on - strand at 136.178 kb on + strand, within GFF418 at 136.220 kb on + strand, within GFF418 at 136.221 kb on - strand, within GFF418 at 136.312 kb on - strand, within GFF418 at 136.341 kb on + strand, within GFF418 at 136.342 kb on - strand, within GFF418 at 136.396 kb on - strand, within GFF418 at 136.396 kb on - strand, within GFF418 at 136.396 kb on - strand, within GFF418 at 136.440 kb on - strand, within GFF418 at 136.528 kb on - strand, within GFF418
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 133,674 - -1.3 133,716 + -0.1 133,716 + -0.3 133,828 + GFF415 0.51 -0.5 133,828 + GFF415 0.51 -0.3 134,045 + GFF416 0.18 -0.5 134,148 - GFF416 0.35 -0.3 134,148 - GFF416 0.35 -1.1 134,208 - GFF416 0.46 -0.4 134,237 + GFF416 0.50 -1.0 134,242 - GFF416 0.51 -0.5 134,242 - GFF416 0.51 -0.6 134,242 - GFF416 0.51 -0.7 134,255 - GFF416 0.53 +1.1 134,306 + GFF416 0.62 -0.3 134,319 - GFF416 0.64 -0.5 134,464 + GFF416 0.89 -1.0 134,475 + +0.2 134,476 - -0.8 134,670 - GFF417 0.13 -0.9 134,710 - GFF417 0.16 -0.3 134,710 - GFF417 0.16 +0.5 134,725 + GFF417 0.17 -2.3 134,796 + GFF417 0.23 -1.6 134,857 + GFF417 0.29 -1.3 134,858 - GFF417 0.29 -1.4 135,011 + GFF417 0.42 -2.8 135,011 + GFF417 0.42 -2.3 135,011 + GFF417 0.42 -2.5 135,012 - GFF417 0.42 -4.0 135,041 + GFF417 0.45 -3.3 135,041 - GFF417 0.45 -1.0 135,108 - GFF417 0.51 -0.1 135,108 - GFF417 0.51 +0.0 135,197 + GFF417 0.58 -2.2 135,326 + GFF417 0.70 -1.3 135,327 - GFF417 0.70 +0.1 135,393 - GFF417 0.75 -1.8 135,430 - GFF417 0.79 -1.3 135,430 - GFF417 0.79 +0.5 135,430 - GFF417 0.79 +0.3 135,542 + GFF417 0.88 +0.9 135,543 - GFF417 0.89 +0.6 135,543 - GFF417 0.89 -1.8 135,579 + -1.9 135,737 - +0.4 135,737 - +0.2 135,820 - +0.3 135,823 + +0.5 135,823 + +0.2 135,824 - -0.1 135,824 - +0.8 135,827 + +0.3 135,827 + +0.3 135,828 - -0.2 135,828 - -0.5 135,828 - +0.7 135,889 + +0.2 135,890 - +0.3 136,023 + -0.1 136,024 - -0.0 136,082 - +0.5 136,178 + GFF418 0.15 +0.1 136,220 + GFF418 0.17 +0.0 136,221 - GFF418 0.17 -0.1 136,312 - GFF418 0.22 +0.0 136,341 + GFF418 0.23 -0.0 136,342 - GFF418 0.23 +0.6 136,396 - GFF418 0.26 -0.7 136,396 - GFF418 0.26 -0.1 136,396 - GFF418 0.26 +0.5 136,440 - GFF418 0.28 -0.7 136,528 - GFF418 0.33 -0.3
Or see this region's nucleotide sequence