Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4169

Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4168 and GFF4169 are separated by 110 nucleotidesGFF4169 and GFF4170 are separated by 56 nucleotidesGFF4170 and GFF4171 are separated by 15 nucleotidesGFF4171 and GFF4172 are separated by 44 nucleotides GFF4168 - Signal recognition particle, subunit Ffh SRP54 (TC 3.A.5.1.1), at 58,934 to 60,295 GFF4168 GFF4169 - hypothetical protein, at 60,406 to 60,552 GFF4169 GFF4170 - SSU ribosomal protein S16p, at 60,609 to 60,857 GFF4170 GFF4171 - 16S rRNA processing protein RimM, at 60,873 to 61,424 GFF4171 GFF4172 - tRNA (Guanine37-N1) -methyltransferase (EC 2.1.1.31), at 61,469 to 62,236 GFF4172 Position (kb) 60 61Strain fitness (log2 ratio) -1 0 1at 60.293 kb on + strandat 60.294 kb on - strandat 60.299 kb on + strandat 60.313 kb on - strandat 60.424 kb on + strand, within GFF4169at 60.424 kb on + strand, within GFF4169at 61.444 kb on + strandat 61.444 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI
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60,293 + +0.4
60,294 - -0.1
60,299 + +0.3
60,313 - +1.0
60,424 + GFF4169 0.12 +0.1
60,424 + GFF4169 0.12 +1.2
61,444 + +1.0
61,444 + -1.2

Or see this region's nucleotide sequence