Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4159 and GFF4160 are separated by 4 nucleotides GFF4160 and GFF4161 are separated by 165 nucleotides GFF4161 and GFF4162 are separated by 148 nucleotides GFF4162 and GFF4163 are separated by 85 nucleotides
GFF4159 - Putative oligoketide cyclase/lipid transport protein, similarity with yeast ubiquinone-binding protein YOL008W, at 51,250 to 51,639
GFF4159
GFF4160 - UPF0125 protein yfjF, at 51,644 to 51,919
GFF4160
GFF4161 - Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex, at 52,085 to 52,423
GFF4161
GFF4162 - DNA repair protein RecN, at 52,572 to 54,233
GFF4162
GFF4163 - NAD kinase (EC 2.7.1.23), at 54,319 to 55,197
GFF4163
Position (kb) 52
53
54
55 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 51.680 kb on + strand, within GFF4160 at 51.781 kb on + strand, within GFF4160 at 51.786 kb on + strand, within GFF4160 at 51.787 kb on - strand, within GFF4160 at 51.794 kb on + strand, within GFF4160 at 51.882 kb on + strand, within GFF4160 at 51.883 kb on - strand, within GFF4160 at 51.883 kb on - strand, within GFF4160 at 51.913 kb on + strand at 52.060 kb on - strand at 52.087 kb on - strand at 52.200 kb on + strand, within GFF4161 at 52.201 kb on - strand, within GFF4161 at 52.216 kb on + strand, within GFF4161 at 52.304 kb on - strand, within GFF4161 at 52.304 kb on - strand, within GFF4161 at 52.304 kb on - strand, within GFF4161 at 52.310 kb on - strand, within GFF4161 at 52.314 kb on + strand, within GFF4161 at 52.314 kb on + strand, within GFF4161 at 52.314 kb on + strand, within GFF4161 at 52.467 kb on - strand at 52.470 kb on - strand at 52.470 kb on - strand at 52.470 kb on - strand at 52.473 kb on - strand at 52.487 kb on - strand at 52.504 kb on - strand at 53.180 kb on + strand, within GFF4162 at 53.497 kb on - strand, within GFF4162 at 53.634 kb on - strand, within GFF4162 at 53.789 kb on - strand, within GFF4162 at 53.789 kb on - strand, within GFF4162 at 53.916 kb on - strand, within GFF4162 at 54.132 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 51,680 + GFF4160 0.13 -1.0 51,781 + GFF4160 0.50 -0.7 51,786 + GFF4160 0.51 -0.9 51,787 - GFF4160 0.52 -2.2 51,794 + GFF4160 0.54 +0.4 51,882 + GFF4160 0.86 -0.2 51,883 - GFF4160 0.87 -0.2 51,883 - GFF4160 0.87 -1.2 51,913 + +0.1 52,060 - -0.6 52,087 - +0.2 52,200 + GFF4161 0.34 -0.4 52,201 - GFF4161 0.34 -0.2 52,216 + GFF4161 0.39 -0.1 52,304 - GFF4161 0.65 -0.1 52,304 - GFF4161 0.65 +1.2 52,304 - GFF4161 0.65 -0.7 52,310 - GFF4161 0.66 -0.6 52,314 + GFF4161 0.68 -1.2 52,314 + GFF4161 0.68 -0.8 52,314 + GFF4161 0.68 +0.1 52,467 - +0.4 52,470 - +1.6 52,470 - +0.3 52,470 - +0.4 52,473 - +1.0 52,487 - -0.0 52,504 - +0.6 53,180 + GFF4162 0.37 -2.3 53,497 - GFF4162 0.56 -4.1 53,634 - GFF4162 0.64 -2.2 53,789 - GFF4162 0.73 -2.7 53,789 - GFF4162 0.73 -1.7 53,916 - GFF4162 0.81 -0.9 54,132 - -0.9
Or see this region's nucleotide sequence