Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4090 and GFF4091 are separated by 110 nucleotides GFF4091 and GFF4092 are separated by 21 nucleotides GFF4092 and GFF4093 are separated by 405 nucleotides
GFF4090 - Periplasmic fimbrial chaperone protein, at 48,155 to 48,952
GFF4090
GFF4091 - FIG034929: Fimbriae usher protein SafC, at 49,063 to 51,486
GFF4091
GFF4092 - Putative fimbrial structural subunit, at 51,508 to 51,978
GFF4092
GFF4093 - Polysaccharide deacetylase, at 52,384 to 53,205
GFF4093
Position (kb)
49
50
51
52 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 48.152 kb on - strand at 48.268 kb on + strand, within GFF4090 at 48.280 kb on - strand, within GFF4090 at 48.313 kb on + strand, within GFF4090 at 48.574 kb on + strand, within GFF4090 at 48.665 kb on - strand, within GFF4090 at 48.817 kb on + strand, within GFF4090 at 48.817 kb on + strand, within GFF4090 at 48.830 kb on + strand, within GFF4090 at 48.830 kb on + strand, within GFF4090 at 48.830 kb on + strand, within GFF4090 at 48.831 kb on - strand, within GFF4090 at 48.863 kb on - strand, within GFF4090 at 48.902 kb on - strand at 48.947 kb on - strand at 49.092 kb on + strand at 49.092 kb on + strand at 49.092 kb on + strand at 49.093 kb on - strand at 49.116 kb on - strand at 49.132 kb on + strand at 49.159 kb on - strand at 49.280 kb on - strand at 49.477 kb on + strand, within GFF4091 at 49.515 kb on + strand, within GFF4091 at 49.515 kb on + strand, within GFF4091 at 49.566 kb on + strand, within GFF4091 at 49.568 kb on + strand, within GFF4091 at 49.642 kb on + strand, within GFF4091 at 49.643 kb on - strand, within GFF4091 at 49.643 kb on - strand, within GFF4091 at 49.644 kb on + strand, within GFF4091 at 49.645 kb on - strand, within GFF4091 at 49.645 kb on - strand, within GFF4091 at 49.645 kb on - strand, within GFF4091 at 49.648 kb on + strand, within GFF4091 at 49.666 kb on + strand, within GFF4091 at 49.666 kb on + strand, within GFF4091 at 49.666 kb on + strand, within GFF4091 at 49.719 kb on - strand, within GFF4091 at 49.719 kb on - strand, within GFF4091 at 49.923 kb on + strand, within GFF4091 at 49.923 kb on + strand, within GFF4091 at 49.965 kb on + strand, within GFF4091 at 50.097 kb on + strand, within GFF4091 at 50.098 kb on - strand, within GFF4091 at 50.107 kb on + strand, within GFF4091 at 50.107 kb on + strand, within GFF4091 at 50.107 kb on + strand, within GFF4091 at 50.107 kb on + strand, within GFF4091 at 50.108 kb on - strand, within GFF4091 at 50.144 kb on + strand, within GFF4091 at 50.362 kb on - strand, within GFF4091 at 50.448 kb on + strand, within GFF4091 at 50.448 kb on + strand, within GFF4091 at 50.654 kb on + strand, within GFF4091 at 50.655 kb on - strand, within GFF4091 at 50.655 kb on - strand, within GFF4091 at 50.700 kb on + strand, within GFF4091 at 50.705 kb on + strand, within GFF4091 at 50.706 kb on - strand, within GFF4091 at 50.712 kb on + strand, within GFF4091 at 50.712 kb on + strand, within GFF4091 at 50.713 kb on - strand, within GFF4091 at 50.815 kb on + strand, within GFF4091 at 50.844 kb on + strand, within GFF4091 at 50.891 kb on + strand, within GFF4091 at 50.902 kb on + strand, within GFF4091 at 50.904 kb on + strand, within GFF4091 at 50.904 kb on + strand, within GFF4091 at 50.904 kb on + strand, within GFF4091 at 50.908 kb on + strand, within GFF4091 at 50.952 kb on + strand, within GFF4091 at 50.952 kb on + strand, within GFF4091 at 50.952 kb on + strand, within GFF4091 at 50.952 kb on + strand, within GFF4091 at 50.954 kb on + strand, within GFF4091 at 50.954 kb on + strand, within GFF4091 at 50.954 kb on + strand, within GFF4091 at 50.955 kb on - strand, within GFF4091 at 50.955 kb on - strand, within GFF4091 at 51.120 kb on + strand, within GFF4091 at 51.120 kb on + strand, within GFF4091 at 51.545 kb on - strand at 51.547 kb on - strand at 51.557 kb on + strand, within GFF4092 at 51.620 kb on + strand, within GFF4092 at 51.621 kb on - strand, within GFF4092 at 51.626 kb on + strand, within GFF4092 at 51.972 kb on + strand at 51.973 kb on - strand at 51.995 kb on + strand at 52.413 kb on - strand at 52.455 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 48,152 - -1.0 48,268 + GFF4090 0.14 -0.4 48,280 - GFF4090 0.16 -0.1 48,313 + GFF4090 0.20 +0.6 48,574 + GFF4090 0.53 +0.5 48,665 - GFF4090 0.64 +0.1 48,817 + GFF4090 0.83 +0.8 48,817 + GFF4090 0.83 +0.6 48,830 + GFF4090 0.85 -0.6 48,830 + GFF4090 0.85 +0.2 48,830 + GFF4090 0.85 -0.9 48,831 - GFF4090 0.85 -0.0 48,863 - GFF4090 0.89 -0.9 48,902 - -0.4 48,947 - -0.1 49,092 + +0.0 49,092 + +1.0 49,092 + +0.4 49,093 - +1.1 49,116 - -0.2 49,132 + -0.5 49,159 - -0.3 49,280 - -1.0 49,477 + GFF4091 0.17 -1.0 49,515 + GFF4091 0.19 -2.7 49,515 + GFF4091 0.19 -1.2 49,566 + GFF4091 0.21 +1.1 49,568 + GFF4091 0.21 +0.6 49,642 + GFF4091 0.24 -0.4 49,643 - GFF4091 0.24 -0.4 49,643 - GFF4091 0.24 +0.4 49,644 + GFF4091 0.24 -0.3 49,645 - GFF4091 0.24 +0.2 49,645 - GFF4091 0.24 -0.2 49,645 - GFF4091 0.24 -0.2 49,648 + GFF4091 0.24 +0.8 49,666 + GFF4091 0.25 +0.2 49,666 + GFF4091 0.25 +0.8 49,666 + GFF4091 0.25 +0.3 49,719 - GFF4091 0.27 +0.6 49,719 - GFF4091 0.27 -0.0 49,923 + GFF4091 0.35 +0.1 49,923 + GFF4091 0.35 +0.1 49,965 + GFF4091 0.37 +0.5 50,097 + GFF4091 0.43 +0.6 50,098 - GFF4091 0.43 +0.9 50,107 + GFF4091 0.43 -0.8 50,107 + GFF4091 0.43 +0.9 50,107 + GFF4091 0.43 +0.0 50,107 + GFF4091 0.43 +0.6 50,108 - GFF4091 0.43 -0.1 50,144 + GFF4091 0.45 -0.3 50,362 - GFF4091 0.54 -0.2 50,448 + GFF4091 0.57 +1.0 50,448 + GFF4091 0.57 +0.6 50,654 + GFF4091 0.66 -0.1 50,655 - GFF4091 0.66 -0.6 50,655 - GFF4091 0.66 -0.1 50,700 + GFF4091 0.68 +0.0 50,705 + GFF4091 0.68 +0.6 50,706 - GFF4091 0.68 +0.4 50,712 + GFF4091 0.68 +0.1 50,712 + GFF4091 0.68 +0.4 50,713 - GFF4091 0.68 +0.4 50,815 + GFF4091 0.72 -0.2 50,844 + GFF4091 0.73 +0.7 50,891 + GFF4091 0.75 +0.4 50,902 + GFF4091 0.76 +0.8 50,904 + GFF4091 0.76 -0.5 50,904 + GFF4091 0.76 -1.8 50,904 + GFF4091 0.76 +1.0 50,908 + GFF4091 0.76 +0.9 50,952 + GFF4091 0.78 +0.4 50,952 + GFF4091 0.78 +0.2 50,952 + GFF4091 0.78 -0.1 50,952 + GFF4091 0.78 -0.4 50,954 + GFF4091 0.78 -0.2 50,954 + GFF4091 0.78 +0.5 50,954 + GFF4091 0.78 +0.1 50,955 - GFF4091 0.78 -1.3 50,955 - GFF4091 0.78 +0.1 51,120 + GFF4091 0.85 +0.4 51,120 + GFF4091 0.85 +0.4 51,545 - -0.1 51,547 - -0.9 51,557 + GFF4092 0.10 -0.2 51,620 + GFF4092 0.24 -1.1 51,621 - GFF4092 0.24 +0.4 51,626 + GFF4092 0.25 -4.0 51,972 + -0.3 51,973 - +0.1 51,995 + +0.5 52,413 - -0.1 52,455 - +0.1
Or see this region's nucleotide sequence