Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4065 and GFF4066 are separated by 4 nucleotides GFF4066 and GFF4067 are separated by 277 nucleotides GFF4067 and GFF4068 are separated by 33 nucleotides
GFF4065 - Outer membrane protein ImpK/VasF, OmpA/MotB domain, at 26,521 to 27,825
GFF4065
GFF4066 - Putative inner membrane protein, at 27,830 to 28,603
GFF4066
GFF4067 - putative Shiga-like toxin A subunit, at 28,881 to 29,237
GFF4067
GFF4068 - IcmF-related protein, at 29,271 to 33,140
GFF4068
Position (kb) 27
28
29 Strain fitness (log2 ratio) -1
0
1
2 at 26.831 kb on + strand, within GFF4065 at 26.832 kb on - strand, within GFF4065 at 26.873 kb on + strand, within GFF4065 at 26.889 kb on + strand, within GFF4065 at 27.051 kb on - strand, within GFF4065 at 27.060 kb on + strand, within GFF4065 at 27.065 kb on - strand, within GFF4065 at 27.136 kb on - strand, within GFF4065 at 27.307 kb on + strand, within GFF4065 at 27.351 kb on + strand, within GFF4065 at 27.352 kb on - strand, within GFF4065 at 27.352 kb on - strand, within GFF4065 at 27.478 kb on - strand, within GFF4065 at 27.656 kb on + strand, within GFF4065 at 27.874 kb on - strand at 27.914 kb on + strand, within GFF4066 at 27.921 kb on + strand, within GFF4066 at 27.922 kb on - strand, within GFF4066 at 27.954 kb on + strand, within GFF4066 at 27.955 kb on - strand, within GFF4066 at 27.955 kb on - strand, within GFF4066 at 27.955 kb on - strand, within GFF4066 at 28.037 kb on - strand, within GFF4066 at 28.037 kb on - strand, within GFF4066 at 28.100 kb on + strand, within GFF4066 at 28.100 kb on + strand, within GFF4066 at 28.100 kb on + strand, within GFF4066 at 28.101 kb on - strand, within GFF4066 at 28.101 kb on - strand, within GFF4066 at 28.101 kb on - strand, within GFF4066 at 28.165 kb on + strand, within GFF4066 at 28.166 kb on - strand, within GFF4066 at 28.167 kb on + strand, within GFF4066 at 28.168 kb on - strand, within GFF4066 at 28.174 kb on - strand, within GFF4066 at 28.240 kb on - strand, within GFF4066 at 28.402 kb on + strand, within GFF4066 at 28.421 kb on + strand, within GFF4066 at 28.509 kb on - strand, within GFF4066 at 28.654 kb on - strand at 29.475 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 26,831 + GFF4065 0.24 -0.5 26,832 - GFF4065 0.24 +0.6 26,873 + GFF4065 0.27 +0.4 26,889 + GFF4065 0.28 +0.4 27,051 - GFF4065 0.41 -0.1 27,060 + GFF4065 0.41 +0.0 27,065 - GFF4065 0.42 +0.2 27,136 - GFF4065 0.47 +0.6 27,307 + GFF4065 0.60 -0.1 27,351 + GFF4065 0.64 +0.6 27,352 - GFF4065 0.64 +0.2 27,352 - GFF4065 0.64 +0.2 27,478 - GFF4065 0.73 +0.9 27,656 + GFF4065 0.87 +0.5 27,874 - +0.2 27,914 + GFF4066 0.11 +0.4 27,921 + GFF4066 0.12 +0.2 27,922 - GFF4066 0.12 -0.4 27,954 + GFF4066 0.16 -0.2 27,955 - GFF4066 0.16 -0.9 27,955 - GFF4066 0.16 +0.5 27,955 - GFF4066 0.16 +2.1 28,037 - GFF4066 0.27 -0.0 28,037 - GFF4066 0.27 -0.7 28,100 + GFF4066 0.35 -1.2 28,100 + GFF4066 0.35 -0.2 28,100 + GFF4066 0.35 -0.1 28,101 - GFF4066 0.35 -0.3 28,101 - GFF4066 0.35 +0.4 28,101 - GFF4066 0.35 -1.6 28,165 + GFF4066 0.43 -0.4 28,166 - GFF4066 0.43 -0.2 28,167 + GFF4066 0.44 +0.0 28,168 - GFF4066 0.44 -0.3 28,174 - GFF4066 0.44 +0.3 28,240 - GFF4066 0.53 -0.6 28,402 + GFF4066 0.74 -1.7 28,421 + GFF4066 0.76 -0.6 28,509 - GFF4066 0.88 -0.7 28,654 - -0.1 29,475 - -0.1
Or see this region's nucleotide sequence