Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4018 and GFF4019 are separated by 107 nucleotides GFF4019 and GFF4020 are separated by 46 nucleotides GFF4020 and GFF4021 are separated by 119 nucleotides GFF4021 and GFF4022 are separated by 297 nucleotides GFF4022 and GFF4023 overlap by 4 nucleotides
GFF4018 - Flagellar M-ring protein FliF, at 286,632 to 288,227
GFF4018
GFF4019 - FIG00640941: hypothetical protein, at 288,335 to 288,484
GFF4019
GFF4020 - Flagellar hook-basal body complex protein FliE, at 288,531 to 288,845
GFF4020
GFF4021 - FIG01200701: possible membrane protein, at 288,965 to 289,318
GFF4021
GFF4022 - UPF0033 protein YedF, at 289,616 to 289,849
GFF4022
GFF4023 - Putative transport system permease protein, at 289,846 to 291,051
GFF4023
Position (kb) 288
289
290 Strain fitness (log2 ratio) -1
0
1 at 287.997 kb on - strand, within GFF4018 at 288.149 kb on + strand at 288.149 kb on + strand at 288.541 kb on + strand at 288.563 kb on + strand, within GFF4020 at 288.572 kb on - strand, within GFF4020 at 288.644 kb on - strand, within GFF4020 at 288.644 kb on - strand, within GFF4020 at 288.644 kb on - strand, within GFF4020 at 288.891 kb on + strand at 289.181 kb on - strand, within GFF4021 at 289.930 kb on + strand at 289.941 kb on - strand at 289.986 kb on + strand, within GFF4023 at 290.085 kb on - strand, within GFF4023 at 290.200 kb on - strand, within GFF4023 at 290.228 kb on + strand, within GFF4023 at 290.270 kb on + strand, within GFF4023
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 287,997 - GFF4018 0.86 -1.1 288,149 + -0.0 288,149 + -0.1 288,541 + +0.1 288,563 + GFF4020 0.10 +0.2 288,572 - GFF4020 0.13 -0.7 288,644 - GFF4020 0.36 -0.6 288,644 - GFF4020 0.36 +0.1 288,644 - GFF4020 0.36 -0.3 288,891 + +0.1 289,181 - GFF4021 0.61 -0.6 289,930 + -1.2 289,941 - -0.1 289,986 + GFF4023 0.12 -0.4 290,085 - GFF4023 0.20 -0.1 290,200 - GFF4023 0.29 +0.1 290,228 + GFF4023 0.32 +0.3 290,270 + GFF4023 0.35 -0.4
Or see this region's nucleotide sequence