Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3999 and GFF4000 are separated by 127 nucleotides GFF4000 and GFF4001 are separated by 164 nucleotides GFF4001 and GFF4002 overlap by 22 nucleotides
GFF3999 - Putative inner membrane protein, at 272,060 to 272,971
GFF3999
GFF4000 - FIG00638072: hypothetical protein, at 273,099 to 273,233
GFF4000
GFF4001 - FIG00638997: hypothetical protein, at 273,398 to 275,110
GFF4001
GFF4002 - Putative mannosyl-3-phosphoglycerate phosphatase (EC 3.1.3.70), at 275,089 to 275,904
GFF4002
Position (kb) 273
274
275
276 Strain fitness (log2 ratio) -2
-1
0
1 at 272.484 kb on + strand, within GFF3999 at 272.621 kb on + strand, within GFF3999 at 272.635 kb on + strand, within GFF3999 at 272.639 kb on - strand, within GFF3999 at 272.817 kb on + strand, within GFF3999 at 272.905 kb on + strand at 272.977 kb on + strand at 273.047 kb on + strand at 273.048 kb on - strand at 273.310 kb on + strand at 273.328 kb on + strand at 273.517 kb on + strand at 273.517 kb on + strand at 273.537 kb on + strand at 273.552 kb on - strand at 273.649 kb on + strand, within GFF4001 at 273.649 kb on + strand, within GFF4001 at 273.924 kb on - strand, within GFF4001 at 273.924 kb on - strand, within GFF4001 at 273.979 kb on + strand, within GFF4001 at 273.996 kb on - strand, within GFF4001 at 274.001 kb on + strand, within GFF4001 at 274.086 kb on - strand, within GFF4001 at 274.185 kb on + strand, within GFF4001 at 274.241 kb on + strand, within GFF4001 at 274.242 kb on - strand, within GFF4001 at 274.323 kb on + strand, within GFF4001 at 274.379 kb on + strand, within GFF4001 at 274.379 kb on + strand, within GFF4001 at 274.438 kb on - strand, within GFF4001 at 274.438 kb on - strand, within GFF4001 at 274.449 kb on + strand, within GFF4001 at 274.449 kb on + strand, within GFF4001 at 274.472 kb on - strand, within GFF4001 at 274.472 kb on - strand, within GFF4001 at 274.708 kb on - strand, within GFF4001 at 274.896 kb on + strand, within GFF4001 at 274.897 kb on - strand, within GFF4001 at 274.997 kb on + strand at 275.062 kb on + strand at 275.109 kb on - strand at 275.188 kb on + strand, within GFF4002 at 275.590 kb on - strand, within GFF4002 at 275.642 kb on + strand, within GFF4002 at 275.643 kb on - strand, within GFF4002 at 275.643 kb on - strand, within GFF4002 at 275.717 kb on - strand, within GFF4002 at 275.722 kb on + strand, within GFF4002
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 272,484 + GFF3999 0.46 -0.4 272,621 + GFF3999 0.62 -0.3 272,635 + GFF3999 0.63 +1.0 272,639 - GFF3999 0.63 +0.5 272,817 + GFF3999 0.83 +0.1 272,905 + -1.9 272,977 + -0.1 273,047 + -0.0 273,048 - +0.7 273,310 + -1.8 273,328 + -1.6 273,517 + +0.5 273,517 + -0.6 273,537 + +0.2 273,552 - +0.2 273,649 + GFF4001 0.15 +0.4 273,649 + GFF4001 0.15 +0.2 273,924 - GFF4001 0.31 +0.1 273,924 - GFF4001 0.31 +0.4 273,979 + GFF4001 0.34 +0.2 273,996 - GFF4001 0.35 -0.6 274,001 + GFF4001 0.35 +0.2 274,086 - GFF4001 0.40 +0.5 274,185 + GFF4001 0.46 +0.6 274,241 + GFF4001 0.49 -0.5 274,242 - GFF4001 0.49 +0.8 274,323 + GFF4001 0.54 +0.1 274,379 + GFF4001 0.57 +0.2 274,379 + GFF4001 0.57 -0.8 274,438 - GFF4001 0.61 -0.1 274,438 - GFF4001 0.61 +0.3 274,449 + GFF4001 0.61 +0.6 274,449 + GFF4001 0.61 +0.5 274,472 - GFF4001 0.63 +0.6 274,472 - GFF4001 0.63 +0.0 274,708 - GFF4001 0.76 +0.6 274,896 + GFF4001 0.87 -0.9 274,897 - GFF4001 0.88 +0.5 274,997 + -0.2 275,062 + +0.9 275,109 - +0.3 275,188 + GFF4002 0.12 +0.3 275,590 - GFF4002 0.61 +0.2 275,642 + GFF4002 0.68 -0.1 275,643 - GFF4002 0.68 -0.1 275,643 - GFF4002 0.68 +0.1 275,717 - GFF4002 0.77 +0.6 275,722 + GFF4002 0.78 -0.3
Or see this region's nucleotide sequence