Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3974 and GFF3975 are separated by 79 nucleotides GFF3975 and GFF3976 are separated by 319 nucleotides GFF3976 and GFF3977 are separated by 44 nucleotides GFF3977 and GFF3978 are separated by 30 nucleotides GFF3978 and GFF3979 are separated by 56 nucleotides
GFF3974 - AMP nucleosidase (EC 3.2.2.4), at 252,092 to 253,546
GFF3974
GFF3975 - hypothetical protein, at 253,626 to 253,748
GFF3975
GFF3976 - FIG01045899: hypothetical protein, at 254,068 to 254,241
GFF3976
GFF3977 - hypothetical protein, at 254,286 to 254,420
GFF3977
GFF3978 - Putative periplasmic protein, at 254,451 to 256,181
GFF3978
GFF3979 - FIG01057005: hypothetical protein, at 256,238 to 258,406
GFF3979
Position (kb) 254
255
256
257 Strain fitness (log2 ratio) -1
0
1 at 253.592 kb on - strand at 253.596 kb on - strand at 253.603 kb on - strand at 253.716 kb on + strand, within GFF3975 at 253.797 kb on + strand at 253.834 kb on - strand at 253.886 kb on - strand at 253.887 kb on + strand at 253.887 kb on + strand at 253.887 kb on + strand at 253.888 kb on - strand at 254.066 kb on - strand at 254.071 kb on + strand at 254.071 kb on + strand at 254.487 kb on - strand at 254.489 kb on - strand at 254.636 kb on + strand, within GFF3978 at 254.764 kb on + strand, within GFF3978 at 254.983 kb on - strand, within GFF3978 at 254.983 kb on - strand, within GFF3978 at 255.134 kb on - strand, within GFF3978 at 255.440 kb on + strand, within GFF3978 at 255.454 kb on + strand, within GFF3978 at 255.491 kb on - strand, within GFF3978 at 255.518 kb on + strand, within GFF3978 at 255.627 kb on - strand, within GFF3978 at 255.802 kb on + strand, within GFF3978 at 256.235 kb on + strand at 256.239 kb on + strand at 256.362 kb on + strand at 256.378 kb on + strand at 256.379 kb on - strand at 256.429 kb on - strand at 256.530 kb on + strand, within GFF3979 at 256.578 kb on + strand, within GFF3979 at 256.579 kb on - strand, within GFF3979 at 256.677 kb on + strand, within GFF3979 at 256.693 kb on - strand, within GFF3979 at 256.749 kb on - strand, within GFF3979 at 256.794 kb on - strand, within GFF3979 at 256.859 kb on + strand, within GFF3979 at 256.860 kb on - strand, within GFF3979 at 256.883 kb on - strand, within GFF3979 at 257.007 kb on + strand, within GFF3979 at 257.008 kb on - strand, within GFF3979 at 257.039 kb on - strand, within GFF3979 at 257.066 kb on - strand, within GFF3979
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 253,592 - -0.4 253,596 - +0.3 253,603 - -0.1 253,716 + GFF3975 0.73 +0.5 253,797 + +0.2 253,834 - +0.2 253,886 - -0.3 253,887 + -0.2 253,887 + +0.6 253,887 + -1.0 253,888 - +0.0 254,066 - +0.0 254,071 + +0.0 254,071 + +0.5 254,487 - -0.3 254,489 - -0.2 254,636 + GFF3978 0.11 +0.2 254,764 + GFF3978 0.18 +1.2 254,983 - GFF3978 0.31 +0.6 254,983 - GFF3978 0.31 +0.1 255,134 - GFF3978 0.39 -0.5 255,440 + GFF3978 0.57 +0.3 255,454 + GFF3978 0.58 +0.3 255,491 - GFF3978 0.60 +0.6 255,518 + GFF3978 0.62 +0.2 255,627 - GFF3978 0.68 +0.2 255,802 + GFF3978 0.78 -0.6 256,235 + -0.3 256,239 + -0.9 256,362 + -0.0 256,378 + -0.4 256,379 - -0.4 256,429 - +0.1 256,530 + GFF3979 0.13 -0.1 256,578 + GFF3979 0.16 +0.4 256,579 - GFF3979 0.16 +0.2 256,677 + GFF3979 0.20 -0.4 256,693 - GFF3979 0.21 +0.3 256,749 - GFF3979 0.24 +0.2 256,794 - GFF3979 0.26 +0.2 256,859 + GFF3979 0.29 -0.1 256,860 - GFF3979 0.29 +0.0 256,883 - GFF3979 0.30 -0.1 257,007 + GFF3979 0.35 +1.7 257,008 - GFF3979 0.36 -0.2 257,039 - GFF3979 0.37 +0.2 257,066 - GFF3979 0.38 -0.0
Or see this region's nucleotide sequence