Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3970 and GFF4032 are separated by 226 nucleotides GFF4032 and GFF3971 are separated by 90 nucleotides GFF3971 and GFF4033 are separated by 140 nucleotides
GFF3970 - L,D-transpeptidase YbiS, at 247,628 to 248,557
GFF3970
GFF4032 - tRNA-Asn-GTT, at 248,784 to 248,859
GFF4032
GFF3971 - FIG01069516: hypothetical protein, at 248,950 to 250,476
GFF3971
GFF4033 - tRNA-Asn-GTT, at 250,617 to 250,692
GFF4033
Position (kb) 248
249
250
251 Strain fitness (log2 ratio) -1
0
1 at 247.986 kb on + strand, within GFF3970 at 247.990 kb on + strand, within GFF3970 at 248.105 kb on - strand, within GFF3970 at 248.173 kb on - strand, within GFF3970 at 248.173 kb on - strand, within GFF3970 at 248.174 kb on + strand, within GFF3970 at 248.174 kb on + strand, within GFF3970 at 248.175 kb on - strand, within GFF3970 at 248.176 kb on + strand, within GFF3970 at 248.188 kb on - strand, within GFF3970 at 248.190 kb on - strand, within GFF3970 at 248.249 kb on - strand, within GFF3970 at 248.479 kb on + strand at 248.560 kb on - strand at 248.560 kb on - strand at 248.594 kb on + strand at 249.041 kb on + strand at 249.157 kb on - strand, within GFF3971 at 249.278 kb on + strand, within GFF3971 at 249.279 kb on - strand, within GFF3971 at 249.279 kb on - strand, within GFF3971 at 249.312 kb on + strand, within GFF3971 at 249.316 kb on - strand, within GFF3971 at 249.434 kb on - strand, within GFF3971 at 249.454 kb on - strand, within GFF3971 at 249.485 kb on + strand, within GFF3971 at 249.631 kb on - strand, within GFF3971 at 249.930 kb on + strand, within GFF3971 at 249.946 kb on - strand, within GFF3971 at 250.011 kb on + strand, within GFF3971 at 250.073 kb on + strand, within GFF3971 at 250.074 kb on - strand, within GFF3971 at 250.094 kb on + strand, within GFF3971 at 250.094 kb on + strand, within GFF3971 at 250.095 kb on - strand, within GFF3971 at 250.200 kb on + strand, within GFF3971 at 250.212 kb on + strand, within GFF3971 at 250.331 kb on - strand at 250.332 kb on + strand at 250.365 kb on - strand at 250.474 kb on + strand at 250.857 kb on + strand at 250.857 kb on + strand at 250.857 kb on + strand at 250.914 kb on + strand at 250.915 kb on - strand at 250.915 kb on - strand at 250.916 kb on + strand at 250.917 kb on - strand at 251.132 kb on + strand at 251.143 kb on + strand at 251.149 kb on + strand at 251.149 kb on + strand at 251.277 kb on - strand at 251.432 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 247,986 + GFF3970 0.38 -0.5 247,990 + GFF3970 0.39 +0.3 248,105 - GFF3970 0.51 +0.2 248,173 - GFF3970 0.59 -0.6 248,173 - GFF3970 0.59 -0.2 248,174 + GFF3970 0.59 -0.0 248,174 + GFF3970 0.59 +0.2 248,175 - GFF3970 0.59 -0.6 248,176 + GFF3970 0.59 +0.2 248,188 - GFF3970 0.60 +0.0 248,190 - GFF3970 0.60 -0.9 248,249 - GFF3970 0.67 -0.1 248,479 + -0.5 248,560 - -1.4 248,560 - +0.4 248,594 + -0.4 249,041 + -0.1 249,157 - GFF3971 0.14 -0.8 249,278 + GFF3971 0.21 +1.4 249,279 - GFF3971 0.22 +0.3 249,279 - GFF3971 0.22 +0.4 249,312 + GFF3971 0.24 -1.2 249,316 - GFF3971 0.24 -0.0 249,434 - GFF3971 0.32 +0.6 249,454 - GFF3971 0.33 -0.2 249,485 + GFF3971 0.35 -0.2 249,631 - GFF3971 0.45 +0.6 249,930 + GFF3971 0.64 +0.4 249,946 - GFF3971 0.65 +0.0 250,011 + GFF3971 0.69 +0.4 250,073 + GFF3971 0.74 +0.2 250,074 - GFF3971 0.74 -0.0 250,094 + GFF3971 0.75 -0.1 250,094 + GFF3971 0.75 -0.9 250,095 - GFF3971 0.75 +0.4 250,200 + GFF3971 0.82 +0.1 250,212 + GFF3971 0.83 +0.7 250,331 - +0.2 250,332 + -0.5 250,365 - +1.5 250,474 + +0.7 250,857 + -0.1 250,857 + -0.1 250,857 + +0.3 250,914 + +0.3 250,915 - +0.4 250,915 - +0.6 250,916 + +0.0 250,917 - +1.3 251,132 + +0.4 251,143 + -0.3 251,149 + +0.9 251,149 + -0.4 251,277 - -0.9 251,432 + -0.8
Or see this region's nucleotide sequence