Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3957 and GFF3958 overlap by 1 nucleotides GFF3958 and GFF3959 overlap by 4 nucleotides GFF3959 and GFF3960 are separated by 2 nucleotides
GFF3957 - Cobalamin biosynthesis protein CbiG, at 237,074 to 238,129
GFF3957
GFF3958 - Cobalt-precorrin-3b C17-methyltransferase, at 238,129 to 238,854
GFF3958
GFF3959 - Cobalt-precorrin-6x reductase (EC 1.3.1.54), at 238,851 to 239,642
GFF3959
GFF3960 - Sirohydrochlorin cobaltochelatase CbiK (EC 4.99.1.3), at 239,645 to 240,439
GFF3960
Position (kb) 238
239 Strain fitness (log2 ratio) -1
0
1
2 at 237.176 kb on - strand at 237.176 kb on - strand at 237.250 kb on + strand, within GFF3957 at 237.269 kb on - strand, within GFF3957 at 237.505 kb on - strand, within GFF3957 at 237.518 kb on - strand, within GFF3957 at 237.650 kb on - strand, within GFF3957 at 237.911 kb on - strand, within GFF3957 at 238.108 kb on + strand at 238.108 kb on + strand at 238.108 kb on + strand at 238.108 kb on + strand at 238.430 kb on + strand, within GFF3958 at 238.443 kb on + strand, within GFF3958 at 238.563 kb on + strand, within GFF3958 at 238.563 kb on + strand, within GFF3958 at 238.603 kb on + strand, within GFF3958 at 238.766 kb on + strand, within GFF3958 at 238.770 kb on + strand, within GFF3958 at 238.807 kb on + strand at 238.808 kb on - strand at 238.843 kb on + strand at 238.935 kb on + strand, within GFF3959 at 238.936 kb on - strand, within GFF3959 at 238.985 kb on + strand, within GFF3959 at 239.065 kb on + strand, within GFF3959 at 239.065 kb on + strand, within GFF3959 at 239.066 kb on - strand, within GFF3959 at 239.102 kb on + strand, within GFF3959 at 239.140 kb on + strand, within GFF3959 at 239.169 kb on - strand, within GFF3959 at 239.254 kb on + strand, within GFF3959 at 239.270 kb on + strand, within GFF3959 at 239.685 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 237,176 - -0.1 237,176 - -0.3 237,250 + GFF3957 0.17 -0.6 237,269 - GFF3957 0.18 -0.1 237,505 - GFF3957 0.41 +0.7 237,518 - GFF3957 0.42 +0.0 237,650 - GFF3957 0.55 +0.5 237,911 - GFF3957 0.79 +0.2 238,108 + +0.2 238,108 + +0.1 238,108 + +0.2 238,108 + -0.3 238,430 + GFF3958 0.41 +0.6 238,443 + GFF3958 0.43 +0.4 238,563 + GFF3958 0.60 +0.7 238,563 + GFF3958 0.60 +1.0 238,603 + GFF3958 0.65 +0.8 238,766 + GFF3958 0.88 +0.2 238,770 + GFF3958 0.88 -0.1 238,807 + -0.0 238,808 - +0.4 238,843 + -1.7 238,935 + GFF3959 0.11 +0.0 238,936 - GFF3959 0.11 -1.0 238,985 + GFF3959 0.17 +1.8 239,065 + GFF3959 0.27 -0.7 239,065 + GFF3959 0.27 +0.3 239,066 - GFF3959 0.27 -0.0 239,102 + GFF3959 0.32 -0.4 239,140 + GFF3959 0.36 +0.5 239,169 - GFF3959 0.40 -0.4 239,254 + GFF3959 0.51 -0.2 239,270 + GFF3959 0.53 -0.2 239,685 - +0.4
Or see this region's nucleotide sequence