Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3911 and GFF3912 overlap by 4 nucleotides GFF3912 and GFF3913 are separated by 102 nucleotides
GFF3911 - Histidinol-phosphate aminotransferase (EC 2.6.1.9), at 191,856 to 192,935
GFF3911
GFF3912 - Histidinol dehydrogenase (EC 1.1.1.23), at 192,932 to 194,236
GFF3912
GFF3913 - ATP phosphoribosyltransferase (EC 2.4.2.17), at 194,339 to 195,238
GFF3913
Position (kb) 192
193
194
195 Strain fitness (log2 ratio) -1
0
1
2 at 191.938 kb on - strand at 192.029 kb on + strand, within GFF3911 at 192.029 kb on + strand, within GFF3911 at 192.216 kb on + strand, within GFF3911 at 192.367 kb on + strand, within GFF3911 at 192.368 kb on - strand, within GFF3911 at 192.368 kb on - strand, within GFF3911 at 192.441 kb on - strand, within GFF3911 at 192.441 kb on - strand, within GFF3911 at 192.441 kb on - strand, within GFF3911 at 192.467 kb on + strand, within GFF3911 at 192.562 kb on - strand, within GFF3911 at 192.590 kb on - strand, within GFF3911 at 192.770 kb on + strand, within GFF3911 at 193.109 kb on - strand, within GFF3912 at 193.116 kb on + strand, within GFF3912 at 193.117 kb on - strand, within GFF3912 at 193.123 kb on - strand, within GFF3912 at 193.123 kb on - strand, within GFF3912 at 193.155 kb on + strand, within GFF3912 at 193.253 kb on + strand, within GFF3912 at 193.269 kb on + strand, within GFF3912 at 193.848 kb on + strand, within GFF3912 at 193.848 kb on + strand, within GFF3912 at 193.849 kb on - strand, within GFF3912 at 194.108 kb on + strand at 194.109 kb on - strand at 194.305 kb on - strand at 194.557 kb on - strand, within GFF3913 at 194.677 kb on + strand, within GFF3913 at 194.678 kb on - strand, within GFF3913 at 194.958 kb on - strand, within GFF3913 at 194.996 kb on + strand, within GFF3913 at 195.025 kb on + strand, within GFF3913 at 195.025 kb on + strand, within GFF3913
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 191,938 - -0.4 192,029 + GFF3911 0.16 +0.8 192,029 + GFF3911 0.16 +1.1 192,216 + GFF3911 0.33 +1.0 192,367 + GFF3911 0.47 +0.2 192,368 - GFF3911 0.47 +0.1 192,368 - GFF3911 0.47 -0.1 192,441 - GFF3911 0.54 +0.2 192,441 - GFF3911 0.54 -0.0 192,441 - GFF3911 0.54 -0.1 192,467 + GFF3911 0.57 +0.0 192,562 - GFF3911 0.65 +0.0 192,590 - GFF3911 0.68 +2.4 192,770 + GFF3911 0.85 +0.1 193,109 - GFF3912 0.14 +0.1 193,116 + GFF3912 0.14 -0.1 193,117 - GFF3912 0.14 -0.4 193,123 - GFF3912 0.15 -0.2 193,123 - GFF3912 0.15 +0.0 193,155 + GFF3912 0.17 +0.7 193,253 + GFF3912 0.25 +0.1 193,269 + GFF3912 0.26 +0.2 193,848 + GFF3912 0.70 -0.9 193,848 + GFF3912 0.70 +0.7 193,849 - GFF3912 0.70 +0.4 194,108 + +0.6 194,109 - +0.3 194,305 - +0.6 194,557 - GFF3913 0.24 +0.4 194,677 + GFF3913 0.38 +0.0 194,678 - GFF3913 0.38 +0.2 194,958 - GFF3913 0.69 -0.5 194,996 + GFF3913 0.73 +0.4 195,025 + GFF3913 0.76 -0.7 195,025 + GFF3913 0.76 +0.9
Or see this region's nucleotide sequence