Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3868 and GFF3869 are separated by 86 nucleotides GFF3869 and GFF3870 are separated by 2 nucleotides GFF3870 and GFF3871 overlap by 4 nucleotides
GFF3868 - Tyrosine-protein kinase Wzc (EC 2.7.10.2), at 143,793 to 145,952
GFF3868
GFF3869 - Putative N-acetylgalactosaminyl-diphosphoundecaprenol glucuronosyltransferase, at 146,039 to 146,881
GFF3869
GFF3870 - Colanic acid biosynthesis acetyltransferase WcaB (EC 2.3.1.-), at 146,884 to 147,372
GFF3870
GFF3871 - Colanic acid biosynthesis glycosyl transferase WcaC, at 147,369 to 148,586
GFF3871
Position (kb) 146
147
148 Strain fitness (log2 ratio) -1
0
1
2 at 145.911 kb on + strand at 145.932 kb on + strand at 145.933 kb on - strand at 145.990 kb on - strand at 146.125 kb on - strand, within GFF3869 at 146.136 kb on - strand, within GFF3869 at 146.231 kb on + strand, within GFF3869 at 146.303 kb on + strand, within GFF3869 at 146.304 kb on - strand, within GFF3869 at 146.399 kb on + strand, within GFF3869 at 146.411 kb on + strand, within GFF3869 at 146.411 kb on + strand, within GFF3869 at 146.488 kb on - strand, within GFF3869 at 146.506 kb on + strand, within GFF3869 at 146.524 kb on + strand, within GFF3869 at 146.525 kb on - strand, within GFF3869 at 146.586 kb on - strand, within GFF3869 at 146.659 kb on + strand, within GFF3869 at 146.660 kb on - strand, within GFF3869 at 146.784 kb on - strand, within GFF3869 at 146.846 kb on - strand at 147.264 kb on + strand, within GFF3870 at 147.264 kb on + strand, within GFF3870 at 147.265 kb on - strand, within GFF3870 at 147.274 kb on - strand, within GFF3870 at 147.853 kb on + strand, within GFF3871 at 148.063 kb on + strand, within GFF3871 at 148.064 kb on - strand, within GFF3871 at 148.193 kb on + strand, within GFF3871 at 148.317 kb on + strand, within GFF3871
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 145,911 + +0.5 145,932 + +0.1 145,933 - +2.1 145,990 - +0.2 146,125 - GFF3869 0.10 +0.2 146,136 - GFF3869 0.12 +0.8 146,231 + GFF3869 0.23 -0.0 146,303 + GFF3869 0.31 -0.6 146,304 - GFF3869 0.31 -0.5 146,399 + GFF3869 0.43 +1.3 146,411 + GFF3869 0.44 -0.1 146,411 + GFF3869 0.44 +0.4 146,488 - GFF3869 0.53 +0.2 146,506 + GFF3869 0.55 -0.3 146,524 + GFF3869 0.58 +0.4 146,525 - GFF3869 0.58 -0.6 146,586 - GFF3869 0.65 +0.3 146,659 + GFF3869 0.74 +0.1 146,660 - GFF3869 0.74 +0.4 146,784 - GFF3869 0.88 -0.2 146,846 - +0.6 147,264 + GFF3870 0.78 -0.3 147,264 + GFF3870 0.78 -0.7 147,265 - GFF3870 0.78 -0.8 147,274 - GFF3870 0.80 -0.4 147,853 + GFF3871 0.40 +0.0 148,063 + GFF3871 0.57 +0.1 148,064 - GFF3871 0.57 +0.2 148,193 + GFF3871 0.68 +0.3 148,317 + GFF3871 0.78 -0.0
Or see this region's nucleotide sequence