Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3804 and GFF3805 are separated by 70 nucleotides GFF3805 and GFF3806 are separated by 2 nucleotides GFF3806 and GFF3807 are separated by 50 nucleotides GFF3807 and GFF3808 are separated by 44 nucleotides GFF3808 and GFF3809 are separated by 48 nucleotides
GFF3804 - tRNA-dihydrouridine synthase C (EC 1.-.-.-), at 73,133 to 74,128
GFF3804
GFF3805 - FIG01045646: hypothetical protein, at 74,199 to 74,396
GFF3805
GFF3806 - FIG01045848: hypothetical protein, at 74,399 to 74,536
GFF3806
GFF3807 - RND efflux system, outer membrane lipoprotein, NodT family, at 74,587 to 76,023
GFF3807
GFF3808 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 76,068 to 76,850
GFF3808
GFF3809 - DedA family inner membrane protein YohD, at 76,899 to 77,468
GFF3809
Position (kb) 74
75
76
77 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 73.730 kb on - strand, within GFF3804 at 73.826 kb on - strand, within GFF3804 at 73.826 kb on - strand, within GFF3804 at 73.905 kb on - strand, within GFF3804 at 73.918 kb on + strand, within GFF3804 at 73.945 kb on - strand, within GFF3804 at 73.947 kb on + strand, within GFF3804 at 73.948 kb on - strand, within GFF3804 at 73.956 kb on - strand, within GFF3804 at 73.957 kb on + strand, within GFF3804 at 73.992 kb on - strand, within GFF3804 at 74.009 kb on - strand, within GFF3804 at 74.028 kb on + strand, within GFF3804 at 74.029 kb on - strand at 74.134 kb on - strand at 74.162 kb on + strand at 74.163 kb on - strand at 74.163 kb on - strand at 74.163 kb on - strand at 74.163 kb on - strand at 75.004 kb on + strand, within GFF3807 at 75.005 kb on - strand, within GFF3807 at 75.005 kb on - strand, within GFF3807 at 75.006 kb on + strand, within GFF3807 at 75.015 kb on + strand, within GFF3807 at 75.051 kb on - strand, within GFF3807 at 75.051 kb on - strand, within GFF3807 at 75.267 kb on + strand, within GFF3807 at 75.268 kb on - strand, within GFF3807 at 75.727 kb on - strand, within GFF3807 at 75.752 kb on + strand, within GFF3807 at 75.753 kb on - strand, within GFF3807 at 75.981 kb on + strand at 76.052 kb on + strand at 76.053 kb on - strand at 76.059 kb on - strand at 76.067 kb on + strand at 76.088 kb on + strand at 76.108 kb on + strand at 76.311 kb on - strand, within GFF3808 at 76.311 kb on - strand, within GFF3808 at 76.347 kb on - strand, within GFF3808 at 76.376 kb on + strand, within GFF3808 at 76.463 kb on + strand, within GFF3808 at 76.464 kb on - strand, within GFF3808 at 76.470 kb on - strand, within GFF3808 at 76.470 kb on - strand, within GFF3808 at 76.521 kb on - strand, within GFF3808 at 76.727 kb on + strand, within GFF3808 at 76.727 kb on + strand, within GFF3808 at 76.728 kb on - strand, within GFF3808 at 76.779 kb on + strand at 76.779 kb on + strand at 76.779 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 73,730 - GFF3804 0.60 -0.3 73,826 - GFF3804 0.70 -0.6 73,826 - GFF3804 0.70 -1.0 73,905 - GFF3804 0.78 -0.2 73,918 + GFF3804 0.79 -0.5 73,945 - GFF3804 0.82 -0.0 73,947 + GFF3804 0.82 -0.4 73,948 - GFF3804 0.82 +0.4 73,956 - GFF3804 0.83 -0.2 73,957 + GFF3804 0.83 +0.2 73,992 - GFF3804 0.86 +0.1 74,009 - GFF3804 0.88 +0.4 74,028 + GFF3804 0.90 -0.5 74,029 - +0.2 74,134 - +0.3 74,162 + -0.5 74,163 - -0.4 74,163 - -0.7 74,163 - +0.2 74,163 - -3.0 75,004 + GFF3807 0.29 -1.6 75,005 - GFF3807 0.29 -1.0 75,005 - GFF3807 0.29 -1.4 75,006 + GFF3807 0.29 +0.5 75,015 + GFF3807 0.30 +0.3 75,051 - GFF3807 0.32 -0.0 75,051 - GFF3807 0.32 +0.1 75,267 + GFF3807 0.47 -0.2 75,268 - GFF3807 0.47 -0.1 75,727 - GFF3807 0.79 -0.5 75,752 + GFF3807 0.81 -0.1 75,753 - GFF3807 0.81 +0.3 75,981 + +0.7 76,052 + -0.2 76,053 - +1.0 76,059 - +0.0 76,067 + -0.2 76,088 + +0.3 76,108 + +0.5 76,311 - GFF3808 0.31 -1.2 76,311 - GFF3808 0.31 +0.2 76,347 - GFF3808 0.36 +0.8 76,376 + GFF3808 0.39 -0.2 76,463 + GFF3808 0.50 +0.0 76,464 - GFF3808 0.51 -0.5 76,470 - GFF3808 0.51 -0.3 76,470 - GFF3808 0.51 +0.0 76,521 - GFF3808 0.58 -0.1 76,727 + GFF3808 0.84 -0.2 76,727 + GFF3808 0.84 -0.1 76,728 - GFF3808 0.84 +0.7 76,779 + +0.4 76,779 + +0.9 76,779 + -0.7
Or see this region's nucleotide sequence