Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3739 and GFF3740 are separated by 13 nucleotides GFF3740 and GFF3741 are separated by 60 nucleotides GFF3741 and GFF3742 are separated by 2 nucleotides GFF3742 and GFF3743 are separated by 455 nucleotides
GFF3739 - Phage portal protein, at 7,324 to 7,653
GFF3739
GFF3740 - Membrane proteins related to metalloendopeptidases, at 7,667 to 8,185
GFF3740
GFF3741 - FIG01047264: hypothetical protein, at 8,246 to 9,487
GFF3741
GFF3742 - FIG01047148: hypothetical protein, at 9,490 to 10,017
GFF3742
GFF3743 - FIG01046717: hypothetical protein, at 10,473 to 10,838
GFF3743
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-2
-1
0
1 at 7.315 kb on + strand at 7.319 kb on + strand at 7.320 kb on - strand at 7.320 kb on - strand at 7.320 kb on - strand at 7.691 kb on + strand at 8.166 kb on + strand at 8.167 kb on - strand at 8.309 kb on - strand at 8.359 kb on + strand at 8.360 kb on - strand at 8.360 kb on - strand at 8.360 kb on - strand at 8.360 kb on - strand at 8.360 kb on - strand at 8.360 kb on - strand at 8.389 kb on + strand, within GFF3741 at 8.469 kb on - strand, within GFF3741 at 8.469 kb on - strand, within GFF3741 at 8.716 kb on + strand, within GFF3741 at 8.716 kb on + strand, within GFF3741 at 8.784 kb on + strand, within GFF3741 at 8.784 kb on + strand, within GFF3741 at 8.785 kb on - strand, within GFF3741 at 8.815 kb on + strand, within GFF3741 at 8.816 kb on - strand, within GFF3741 at 8.881 kb on + strand, within GFF3741 at 8.881 kb on + strand, within GFF3741 at 8.881 kb on + strand, within GFF3741 at 9.037 kb on + strand, within GFF3741 at 9.127 kb on + strand, within GFF3741 at 9.242 kb on - strand, within GFF3741 at 9.300 kb on - strand, within GFF3741 at 9.342 kb on + strand, within GFF3741 at 9.342 kb on + strand, within GFF3741 at 9.342 kb on + strand, within GFF3741 at 9.343 kb on - strand, within GFF3741 at 9.385 kb on + strand at 9.472 kb on + strand at 10.015 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 7,315 + +0.2 7,319 + +0.7 7,320 - -0.3 7,320 - +0.4 7,320 - -0.0 7,691 + -0.4 8,166 + +0.2 8,167 - -0.9 8,309 - -0.5 8,359 + +0.4 8,360 - -0.3 8,360 - -1.1 8,360 - -2.2 8,360 - -0.1 8,360 - +0.8 8,360 - -0.1 8,389 + GFF3741 0.12 -0.4 8,469 - GFF3741 0.18 +0.2 8,469 - GFF3741 0.18 -0.3 8,716 + GFF3741 0.38 +0.3 8,716 + GFF3741 0.38 +0.5 8,784 + GFF3741 0.43 -0.2 8,784 + GFF3741 0.43 -1.1 8,785 - GFF3741 0.43 -0.3 8,815 + GFF3741 0.46 -0.3 8,816 - GFF3741 0.46 +0.5 8,881 + GFF3741 0.51 -0.2 8,881 + GFF3741 0.51 -2.1 8,881 + GFF3741 0.51 +0.3 9,037 + GFF3741 0.64 +0.1 9,127 + GFF3741 0.71 -0.5 9,242 - GFF3741 0.80 -1.0 9,300 - GFF3741 0.85 -0.5 9,342 + GFF3741 0.88 +0.1 9,342 + GFF3741 0.88 -0.6 9,342 + GFF3741 0.88 +0.6 9,343 - GFF3741 0.88 +0.3 9,385 + -1.1 9,472 + -0.5 10,015 + +1.2
Or see this region's nucleotide sequence