Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3714 and GFF3715 are separated by 51 nucleotides GFF3715 and GFF3716 overlap by 4 nucleotides GFF3716 and GFF3717 are separated by 305 nucleotides GFF3717 and GFF3718 are separated by 1 nucleotides
GFF3714 - Phosphoribulokinase (EC 2.7.1.19) homolog, function unknown, at 20,104 to 20,973
GFF3714
GFF3715 - FIG01199774: hypothetical protein, at 21,025 to 21,243
GFF3715
GFF3716 - Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase, at 21,240 to 22,199
GFF3716
GFF3717 - FIG01045846: hypothetical protein, at 22,505 to 22,816
GFF3717
GFF3718 - FIG01045308: hypothetical protein, at 22,818 to 23,039
GFF3718
Position (kb)
21
22
23 Strain fitness (log2 ratio)
-2
-1
0
1 at 20.397 kb on - strand, within GFF3714 at 20.508 kb on + strand, within GFF3714 at 20.508 kb on + strand, within GFF3714 at 20.632 kb on - strand, within GFF3714 at 20.672 kb on + strand, within GFF3714 at 20.673 kb on - strand, within GFF3714 at 20.673 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.688 kb on + strand, within GFF3714 at 20.703 kb on + strand, within GFF3714 at 20.724 kb on + strand, within GFF3714 at 20.730 kb on + strand, within GFF3714 at 20.731 kb on - strand, within GFF3714 at 20.731 kb on - strand, within GFF3714 at 20.779 kb on - strand, within GFF3714 at 20.835 kb on - strand, within GFF3714 at 20.945 kb on - strand at 21.002 kb on - strand at 21.002 kb on - strand at 21.002 kb on - strand at 21.007 kb on - strand at 21.070 kb on + strand, within GFF3715 at 21.162 kb on - strand, within GFF3715 at 21.259 kb on + strand at 21.413 kb on + strand, within GFF3716 at 21.414 kb on - strand, within GFF3716 at 21.437 kb on + strand, within GFF3716 at 21.437 kb on + strand, within GFF3716 at 21.437 kb on + strand, within GFF3716 at 21.482 kb on + strand, within GFF3716 at 21.483 kb on - strand, within GFF3716 at 21.493 kb on + strand, within GFF3716 at 21.493 kb on + strand, within GFF3716 at 21.494 kb on - strand, within GFF3716 at 21.497 kb on - strand, within GFF3716 at 21.648 kb on - strand, within GFF3716 at 21.664 kb on + strand, within GFF3716 at 21.695 kb on - strand, within GFF3716 at 21.698 kb on + strand, within GFF3716 at 21.698 kb on + strand, within GFF3716 at 21.788 kb on + strand, within GFF3716 at 21.788 kb on + strand, within GFF3716 at 21.792 kb on - strand, within GFF3716 at 21.892 kb on + strand, within GFF3716 at 21.892 kb on + strand, within GFF3716 at 21.897 kb on - strand, within GFF3716 at 22.047 kb on - strand, within GFF3716 at 22.049 kb on - strand, within GFF3716 at 22.167 kb on - strand at 22.293 kb on + strand at 22.293 kb on + strand at 22.293 kb on + strand at 22.294 kb on - strand at 22.294 kb on - strand at 22.294 kb on - strand at 22.296 kb on + strand at 22.296 kb on + strand at 22.321 kb on + strand at 22.328 kb on + strand at 22.416 kb on - strand at 22.464 kb on + strand at 22.465 kb on - strand at 22.481 kb on - strand at 22.481 kb on - strand at 22.522 kb on - strand at 22.667 kb on + strand, within GFF3717 at 22.668 kb on - strand, within GFF3717 at 22.701 kb on - strand, within GFF3717 at 22.756 kb on + strand, within GFF3717 at 22.823 kb on + strand at 22.823 kb on + strand at 22.884 kb on - strand, within GFF3718 at 22.887 kb on + strand, within GFF3718 at 22.887 kb on + strand, within GFF3718 at 22.888 kb on - strand, within GFF3718 at 22.898 kb on + strand, within GFF3718 at 22.990 kb on - strand, within GFF3718 at 23.013 kb on - strand, within GFF3718 at 23.064 kb on - strand at 23.078 kb on - strand at 23.126 kb on + strand at 23.126 kb on + strand at 23.127 kb on - strand at 23.132 kb on + strand at 23.132 kb on + strand at 23.132 kb on + strand at 23.133 kb on - strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.136 kb on - strand at 23.136 kb on - strand at 23.178 kb on - strand at 23.178 kb on - strand at 23.187 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 20,397 - GFF3714 0.34 +1.1 20,508 + GFF3714 0.46 -0.1 20,508 + GFF3714 0.46 -0.2 20,632 - GFF3714 0.61 -0.6 20,672 + GFF3714 0.65 +0.4 20,673 - GFF3714 0.65 -0.5 20,673 - GFF3714 0.65 -0.2 20,677 - GFF3714 0.66 -0.2 20,677 - GFF3714 0.66 +0.7 20,677 - GFF3714 0.66 +0.2 20,677 - GFF3714 0.66 -1.0 20,677 - GFF3714 0.66 -0.1 20,677 - GFF3714 0.66 -0.1 20,688 + GFF3714 0.67 +0.2 20,703 + GFF3714 0.69 +0.2 20,724 + GFF3714 0.71 -0.3 20,730 + GFF3714 0.72 -0.1 20,731 - GFF3714 0.72 +0.6 20,731 - GFF3714 0.72 -0.4 20,779 - GFF3714 0.78 +0.3 20,835 - GFF3714 0.84 +0.3 20,945 - +0.9 21,002 - +0.4 21,002 - -0.6 21,002 - +0.4 21,007 - -0.1 21,070 + GFF3715 0.21 +0.1 21,162 - GFF3715 0.63 -0.9 21,259 + +0.2 21,413 + GFF3716 0.18 -1.7 21,414 - GFF3716 0.18 -1.3 21,437 + GFF3716 0.21 +1.1 21,437 + GFF3716 0.21 -0.1 21,437 + GFF3716 0.21 +0.4 21,482 + GFF3716 0.25 -1.4 21,483 - GFF3716 0.25 +0.3 21,493 + GFF3716 0.26 -0.4 21,493 + GFF3716 0.26 +0.7 21,494 - GFF3716 0.26 +0.7 21,497 - GFF3716 0.27 +0.2 21,648 - GFF3716 0.42 +0.6 21,664 + GFF3716 0.44 +0.1 21,695 - GFF3716 0.47 +0.0 21,698 + GFF3716 0.48 +0.0 21,698 + GFF3716 0.48 +0.8 21,788 + GFF3716 0.57 -0.0 21,788 + GFF3716 0.57 +0.2 21,792 - GFF3716 0.57 -0.1 21,892 + GFF3716 0.68 -0.3 21,892 + GFF3716 0.68 -0.1 21,897 - GFF3716 0.68 +0.5 22,047 - GFF3716 0.84 +0.3 22,049 - GFF3716 0.84 +0.5 22,167 - +0.4 22,293 + -1.0 22,293 + +0.7 22,293 + +0.3 22,294 - +0.2 22,294 - -0.3 22,294 - +0.3 22,296 + +0.1 22,296 + +0.4 22,321 + +0.2 22,328 + +0.0 22,416 - +0.2 22,464 + -1.8 22,465 - +0.8 22,481 - -0.3 22,481 - +0.1 22,522 - +1.2 22,667 + GFF3717 0.52 +0.3 22,668 - GFF3717 0.52 +0.8 22,701 - GFF3717 0.63 +1.1 22,756 + GFF3717 0.80 -0.0 22,823 + -0.8 22,823 + -0.3 22,884 - GFF3718 0.30 -0.1 22,887 + GFF3718 0.31 -0.4 22,887 + GFF3718 0.31 -0.8 22,888 - GFF3718 0.32 +0.7 22,898 + GFF3718 0.36 +0.2 22,990 - GFF3718 0.77 -0.2 23,013 - GFF3718 0.88 -0.8 23,064 - +0.5 23,078 - +0.4 23,126 + -0.3 23,126 + +0.2 23,127 - -0.2 23,132 + +0.3 23,132 + -0.7 23,132 + -0.0 23,133 - +0.3 23,135 + +0.7 23,135 + +0.5 23,135 + +0.0 23,135 + -0.3 23,135 + -0.3 23,135 + -0.9 23,135 + -0.0 23,135 + +0.1 23,136 - -0.0 23,136 - +0.1 23,178 - +0.1 23,178 - +0.1 23,187 + +0.7
Or see this region's nucleotide sequence