Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3654 and GFF3655 are separated by 217 nucleotides GFF3655 and GFF3656 are separated by 292 nucleotides GFF3656 and GFF3657 are separated by 9 nucleotides
GFF3654 - Hypothetical MFS-type transporter protein YcaD, at 1,208 to 2,356
GFF3654
GFF3655 - Probable transport protein, at 2,574 to 3,995
GFF3655
GFF3656 - Pyruvate formate-lyase activating enzyme (EC 1.97.1.4), at 4,288 to 4,935
GFF3656
GFF3657 - hypothetical protein, at 4,945 to 5,076
GFF3657
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-1
0
1 at 1.610 kb on + strand, within GFF3654 at 1.611 kb on - strand, within GFF3654 at 1.626 kb on - strand, within GFF3654 at 1.626 kb on - strand, within GFF3654 at 1.627 kb on + strand, within GFF3654 at 1.627 kb on + strand, within GFF3654 at 1.628 kb on - strand, within GFF3654 at 1.732 kb on - strand, within GFF3654 at 1.863 kb on + strand, within GFF3654 at 1.864 kb on - strand, within GFF3654 at 1.881 kb on + strand, within GFF3654 at 1.883 kb on + strand, within GFF3654 at 2.023 kb on - strand, within GFF3654 at 2.191 kb on - strand, within GFF3654 at 2.196 kb on - strand, within GFF3654 at 2.297 kb on + strand at 2.395 kb on - strand at 2.421 kb on + strand at 2.543 kb on + strand at 2.667 kb on + strand at 2.667 kb on + strand at 2.668 kb on - strand at 2.722 kb on - strand, within GFF3655 at 2.733 kb on + strand, within GFF3655 at 2.792 kb on + strand, within GFF3655 at 2.893 kb on + strand, within GFF3655 at 2.949 kb on - strand, within GFF3655 at 3.079 kb on + strand, within GFF3655 at 3.113 kb on + strand, within GFF3655 at 3.138 kb on - strand, within GFF3655 at 3.181 kb on - strand, within GFF3655 at 3.181 kb on - strand, within GFF3655 at 3.182 kb on + strand, within GFF3655 at 3.197 kb on + strand, within GFF3655 at 3.197 kb on + strand, within GFF3655 at 3.197 kb on + strand, within GFF3655 at 3.197 kb on + strand, within GFF3655 at 3.198 kb on - strand, within GFF3655 at 3.241 kb on - strand, within GFF3655 at 3.241 kb on - strand, within GFF3655 at 3.399 kb on + strand, within GFF3655 at 3.399 kb on + strand, within GFF3655 at 3.427 kb on - strand, within GFF3655 at 3.549 kb on + strand, within GFF3655 at 3.565 kb on + strand, within GFF3655 at 3.566 kb on - strand, within GFF3655 at 3.692 kb on + strand, within GFF3655 at 3.787 kb on - strand, within GFF3655 at 3.829 kb on + strand, within GFF3655 at 3.830 kb on - strand, within GFF3655 at 3.830 kb on - strand, within GFF3655 at 4.081 kb on - strand at 4.167 kb on + strand at 4.293 kb on - strand at 4.354 kb on - strand, within GFF3656 at 4.405 kb on - strand, within GFF3656 at 4.425 kb on - strand, within GFF3656 at 4.452 kb on + strand, within GFF3656 at 4.563 kb on - strand, within GFF3656 at 4.842 kb on - strand, within GFF3656 at 4.861 kb on + strand, within GFF3656 at 4.862 kb on - strand, within GFF3656
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 1,610 + GFF3654 0.35 +0.3 1,611 - GFF3654 0.35 +0.4 1,626 - GFF3654 0.36 -0.6 1,626 - GFF3654 0.36 -0.6 1,627 + GFF3654 0.36 +0.2 1,627 + GFF3654 0.36 +0.5 1,628 - GFF3654 0.37 +0.3 1,732 - GFF3654 0.46 -0.4 1,863 + GFF3654 0.57 -0.3 1,864 - GFF3654 0.57 -1.1 1,881 + GFF3654 0.59 +0.6 1,883 + GFF3654 0.59 +0.2 2,023 - GFF3654 0.71 -0.4 2,191 - GFF3654 0.86 +0.6 2,196 - GFF3654 0.86 +0.0 2,297 + +0.6 2,395 - +0.3 2,421 + -0.0 2,543 + +0.4 2,667 + +0.2 2,667 + -0.2 2,668 - -0.5 2,722 - GFF3655 0.10 -0.2 2,733 + GFF3655 0.11 -0.7 2,792 + GFF3655 0.15 -0.2 2,893 + GFF3655 0.22 +0.3 2,949 - GFF3655 0.26 -0.7 3,079 + GFF3655 0.36 -0.0 3,113 + GFF3655 0.38 -1.3 3,138 - GFF3655 0.40 -1.2 3,181 - GFF3655 0.43 -1.4 3,181 - GFF3655 0.43 -0.1 3,182 + GFF3655 0.43 -0.0 3,197 + GFF3655 0.44 -0.0 3,197 + GFF3655 0.44 -0.5 3,197 + GFF3655 0.44 +1.3 3,197 + GFF3655 0.44 +1.0 3,198 - GFF3655 0.44 +0.4 3,241 - GFF3655 0.47 -0.4 3,241 - GFF3655 0.47 -0.7 3,399 + GFF3655 0.58 -0.2 3,399 + GFF3655 0.58 -0.4 3,427 - GFF3655 0.60 -0.3 3,549 + GFF3655 0.69 -0.6 3,565 + GFF3655 0.70 -0.7 3,566 - GFF3655 0.70 +0.2 3,692 + GFF3655 0.79 +0.2 3,787 - GFF3655 0.85 -0.7 3,829 + GFF3655 0.88 -0.5 3,830 - GFF3655 0.88 -0.9 3,830 - GFF3655 0.88 -0.6 4,081 - -0.3 4,167 + +0.2 4,293 - +0.1 4,354 - GFF3656 0.10 +0.4 4,405 - GFF3656 0.18 +0.5 4,425 - GFF3656 0.21 +0.3 4,452 + GFF3656 0.25 -0.2 4,563 - GFF3656 0.42 -0.0 4,842 - GFF3656 0.85 -0.1 4,861 + GFF3656 0.88 +0.1 4,862 - GFF3656 0.89 -0.5
Or see this region's nucleotide sequence