Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3546 and GFF3547 are separated by 13 nucleotides GFF3547 and GFF3548 are separated by 153 nucleotides GFF3548 and GFF3549 are separated by 44 nucleotides
GFF3546 - Crotonobetainyl-CoA dehydrogenase (EC 1.3.99.-), at 298,756 to 299,907
GFF3546
GFF3547 - Coenzyme A transferase, at 299,921 to 301,516
GFF3547
GFF3548 - 3-dehydroquinate dehydratase I (EC 4.2.1.10), at 301,670 to 302,428
GFF3548
GFF3549 - Shikimate/quinate 5-dehydrogenase I beta (EC 1.1.1.282), at 302,473 to 303,339
GFF3549
Position (kb) 299
300
301
302 Strain fitness (log2 ratio) -1
0
1 at 299.019 kb on + strand, within GFF3546 at 299.286 kb on + strand, within GFF3546 at 299.418 kb on - strand, within GFF3546 at 299.418 kb on - strand, within GFF3546 at 299.536 kb on + strand, within GFF3546 at 299.708 kb on + strand, within GFF3546 at 300.393 kb on + strand, within GFF3547 at 300.683 kb on - strand, within GFF3547 at 300.974 kb on - strand, within GFF3547 at 301.686 kb on - strand at 301.828 kb on + strand, within GFF3548 at 301.898 kb on - strand, within GFF3548 at 301.928 kb on - strand, within GFF3548 at 302.040 kb on + strand, within GFF3548 at 302.139 kb on + strand, within GFF3548 at 302.139 kb on + strand, within GFF3548 at 302.139 kb on + strand, within GFF3548 at 302.195 kb on + strand, within GFF3548 at 302.196 kb on - strand, within GFF3548 at 302.246 kb on + strand, within GFF3548 at 302.434 kb on - strand at 302.505 kb on - strand at 302.505 kb on - strand at 302.505 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 299,019 + GFF3546 0.23 -0.4 299,286 + GFF3546 0.46 -0.4 299,418 - GFF3546 0.57 +0.4 299,418 - GFF3546 0.57 -0.5 299,536 + GFF3546 0.68 -0.2 299,708 + GFF3546 0.83 -0.2 300,393 + GFF3547 0.30 +0.9 300,683 - GFF3547 0.48 -0.2 300,974 - GFF3547 0.66 +0.2 301,686 - +0.4 301,828 + GFF3548 0.21 +0.5 301,898 - GFF3548 0.30 -0.4 301,928 - GFF3548 0.34 +0.0 302,040 + GFF3548 0.49 -0.5 302,139 + GFF3548 0.62 -0.8 302,139 + GFF3548 0.62 +0.1 302,139 + GFF3548 0.62 -0.8 302,195 + GFF3548 0.69 -0.3 302,196 - GFF3548 0.69 +1.3 302,246 + GFF3548 0.76 -1.2 302,434 - -0.0 302,505 - +0.2 302,505 - +0.2 302,505 - -0.6
Or see this region's nucleotide sequence