Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3506 and GFF3507 are separated by 12 nucleotides GFF3507 and GFF3508 are separated by 43 nucleotides GFF3508 and GFF3509 are separated by 364 nucleotides GFF3509 and GFF3510 are separated by 101 nucleotides
GFF3506 - 6-phospho-beta-glucosidase (EC 3.2.1.86), at 256,997 to 258,352
GFF3506
GFF3507 - Cellobiose phosphotransferase system YdjC-like protein, at 258,365 to 259,123
GFF3507
GFF3508 - Catalase (EC 1.11.1.6), at 259,167 to 261,419
GFF3508
GFF3509 - Cell division activator cedA, at 261,784 to 262,026
GFF3509
GFF3510 - L-cystine uptake protein TcyP, at 262,128 to 263,519
GFF3510
Position (kb) 259
260
261
262 Strain fitness (log2 ratio) -2
-1
0
1 at 258.360 kb on - strand at 258.365 kb on - strand at 258.441 kb on - strand, within GFF3507 at 258.490 kb on + strand, within GFF3507 at 258.706 kb on - strand, within GFF3507 at 258.751 kb on + strand, within GFF3507 at 258.751 kb on + strand, within GFF3507 at 258.752 kb on - strand, within GFF3507 at 258.752 kb on - strand, within GFF3507 at 258.752 kb on - strand, within GFF3507 at 258.811 kb on - strand, within GFF3507 at 258.881 kb on - strand, within GFF3507 at 258.881 kb on - strand, within GFF3507 at 259.020 kb on - strand, within GFF3507 at 259.035 kb on + strand, within GFF3507 at 259.379 kb on - strand at 259.382 kb on - strand at 259.431 kb on + strand, within GFF3508 at 259.509 kb on + strand, within GFF3508 at 259.617 kb on - strand, within GFF3508 at 259.816 kb on + strand, within GFF3508 at 259.860 kb on - strand, within GFF3508 at 259.862 kb on + strand, within GFF3508 at 259.863 kb on - strand, within GFF3508 at 259.863 kb on - strand, within GFF3508 at 259.863 kb on - strand, within GFF3508 at 259.863 kb on - strand, within GFF3508 at 259.918 kb on + strand, within GFF3508 at 259.975 kb on + strand, within GFF3508 at 260.051 kb on - strand, within GFF3508 at 260.051 kb on - strand, within GFF3508 at 260.106 kb on - strand, within GFF3508 at 260.244 kb on - strand, within GFF3508 at 260.329 kb on - strand, within GFF3508 at 260.893 kb on - strand, within GFF3508 at 261.005 kb on + strand, within GFF3508 at 261.006 kb on - strand, within GFF3508 at 261.006 kb on - strand, within GFF3508 at 261.007 kb on + strand, within GFF3508 at 261.008 kb on - strand, within GFF3508 at 261.122 kb on + strand, within GFF3508 at 261.410 kb on + strand at 261.500 kb on + strand at 261.532 kb on + strand at 261.535 kb on - strand at 261.535 kb on - strand at 261.819 kb on + strand, within GFF3509 at 261.822 kb on + strand, within GFF3509 at 261.826 kb on + strand, within GFF3509 at 261.826 kb on + strand, within GFF3509 at 261.827 kb on - strand, within GFF3509 at 261.828 kb on + strand, within GFF3509 at 261.828 kb on + strand, within GFF3509 at 261.907 kb on - strand, within GFF3509 at 261.910 kb on + strand, within GFF3509 at 261.911 kb on - strand, within GFF3509 at 261.961 kb on - strand, within GFF3509 at 262.127 kb on - strand at 262.129 kb on + strand at 262.175 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 258,360 - -0.1 258,365 - -0.4 258,441 - GFF3507 0.10 -0.5 258,490 + GFF3507 0.16 +1.7 258,706 - GFF3507 0.45 -0.3 258,751 + GFF3507 0.51 +0.2 258,751 + GFF3507 0.51 +0.1 258,752 - GFF3507 0.51 +0.4 258,752 - GFF3507 0.51 -1.8 258,752 - GFF3507 0.51 +0.9 258,811 - GFF3507 0.59 -0.6 258,881 - GFF3507 0.68 +0.1 258,881 - GFF3507 0.68 +0.3 259,020 - GFF3507 0.86 -0.6 259,035 + GFF3507 0.88 +1.1 259,379 - -0.2 259,382 - +0.2 259,431 + GFF3508 0.12 -0.3 259,509 + GFF3508 0.15 +0.3 259,617 - GFF3508 0.20 -0.4 259,816 + GFF3508 0.29 -0.9 259,860 - GFF3508 0.31 +0.0 259,862 + GFF3508 0.31 -0.7 259,863 - GFF3508 0.31 -0.4 259,863 - GFF3508 0.31 +0.6 259,863 - GFF3508 0.31 -0.1 259,863 - GFF3508 0.31 -0.5 259,918 + GFF3508 0.33 +0.2 259,975 + GFF3508 0.36 +0.4 260,051 - GFF3508 0.39 -0.2 260,051 - GFF3508 0.39 -0.7 260,106 - GFF3508 0.42 -1.4 260,244 - GFF3508 0.48 -0.0 260,329 - GFF3508 0.52 -1.0 260,893 - GFF3508 0.77 -0.1 261,005 + GFF3508 0.82 +0.0 261,006 - GFF3508 0.82 -0.4 261,006 - GFF3508 0.82 -0.5 261,007 + GFF3508 0.82 -0.2 261,008 - GFF3508 0.82 -1.3 261,122 + GFF3508 0.87 +0.5 261,410 + -0.2 261,500 + +0.1 261,532 + -0.3 261,535 - +0.5 261,535 - -0.1 261,819 + GFF3509 0.14 +0.6 261,822 + GFF3509 0.16 -0.1 261,826 + GFF3509 0.17 +0.3 261,826 + GFF3509 0.17 +0.5 261,827 - GFF3509 0.18 -0.3 261,828 + GFF3509 0.18 +0.2 261,828 + GFF3509 0.18 +0.4 261,907 - GFF3509 0.51 -0.2 261,910 + GFF3509 0.52 +0.9 261,911 - GFF3509 0.52 +0.8 261,961 - GFF3509 0.73 +0.6 262,127 - +0.5 262,129 + +1.7 262,175 + +1.1
Or see this region's nucleotide sequence