Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3501 and GFF3502 are separated by 300 nucleotides GFF3502 and GFF3503 are separated by 82 nucleotides GFF3503 and GFF3504 are separated by 50 nucleotides GFF3504 and GFF3505 are separated by 10 nucleotides
GFF3501 - Osmotically inducible lipoprotein E precursor, at 253,218 to 253,559
GFF3501
GFF3502 - PTS system, cellobiose-specific IIB component (EC 2.7.1.69), at 253,860 to 254,180
GFF3502
GFF3503 - PTS system, chitobiose-specific IIC component (EC 2.7.1.69), at 254,263 to 255,621
GFF3503
GFF3504 - PTS system, cellobiose-specific IIA component (EC 2.7.1.69), at 255,672 to 256,019
GFF3504
GFF3505 - Transcriptional regulator, AraC family, at 256,030 to 256,872
GFF3505
Position (kb) 254
255
256 Strain fitness (log2 ratio) -2
-1
0
1 at 253.265 kb on + strand, within GFF3501 at 253.266 kb on - strand, within GFF3501 at 253.266 kb on - strand, within GFF3501 at 253.287 kb on + strand, within GFF3501 at 253.288 kb on - strand, within GFF3501 at 253.428 kb on + strand, within GFF3501 at 253.465 kb on - strand, within GFF3501 at 253.558 kb on - strand at 253.558 kb on - strand at 253.566 kb on + strand at 253.567 kb on - strand at 253.879 kb on - strand at 254.023 kb on + strand, within GFF3502 at 254.542 kb on - strand, within GFF3503 at 254.638 kb on - strand, within GFF3503 at 254.655 kb on + strand, within GFF3503 at 254.707 kb on - strand, within GFF3503 at 254.707 kb on - strand, within GFF3503 at 254.710 kb on - strand, within GFF3503 at 254.722 kb on - strand, within GFF3503 at 254.752 kb on - strand, within GFF3503 at 254.914 kb on - strand, within GFF3503 at 254.916 kb on + strand, within GFF3503 at 254.972 kb on - strand, within GFF3503 at 255.163 kb on + strand, within GFF3503 at 255.389 kb on - strand, within GFF3503 at 255.504 kb on + strand at 255.694 kb on + strand at 255.695 kb on - strand at 255.848 kb on - strand, within GFF3504 at 256.113 kb on + strand at 256.117 kb on + strand, within GFF3505 at 256.118 kb on - strand, within GFF3505 at 256.119 kb on + strand, within GFF3505 at 256.160 kb on - strand, within GFF3505 at 256.163 kb on - strand, within GFF3505 at 256.192 kb on + strand, within GFF3505 at 256.407 kb on + strand, within GFF3505 at 256.487 kb on - strand, within GFF3505
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 253,265 + GFF3501 0.14 +0.6 253,266 - GFF3501 0.14 +0.1 253,266 - GFF3501 0.14 -1.3 253,287 + GFF3501 0.20 -0.9 253,288 - GFF3501 0.20 -1.6 253,428 + GFF3501 0.61 +0.2 253,465 - GFF3501 0.72 +0.2 253,558 - +0.6 253,558 - +0.3 253,566 + +0.6 253,567 - -0.7 253,879 - +0.4 254,023 + GFF3502 0.51 +0.6 254,542 - GFF3503 0.21 -0.4 254,638 - GFF3503 0.28 +0.2 254,655 + GFF3503 0.29 +0.2 254,707 - GFF3503 0.33 -1.2 254,707 - GFF3503 0.33 +0.1 254,710 - GFF3503 0.33 -0.2 254,722 - GFF3503 0.34 -1.8 254,752 - GFF3503 0.36 +0.1 254,914 - GFF3503 0.48 -0.9 254,916 + GFF3503 0.48 -0.1 254,972 - GFF3503 0.52 -1.1 255,163 + GFF3503 0.66 -1.6 255,389 - GFF3503 0.83 -0.5 255,504 + -0.0 255,694 + -0.8 255,695 - +0.5 255,848 - GFF3504 0.51 -0.0 256,113 + -0.7 256,117 + GFF3505 0.10 +0.5 256,118 - GFF3505 0.10 +0.3 256,119 + GFF3505 0.11 +1.1 256,160 - GFF3505 0.15 -0.3 256,163 - GFF3505 0.16 +0.3 256,192 + GFF3505 0.19 +0.6 256,407 + GFF3505 0.45 -0.3 256,487 - GFF3505 0.54 +1.0
Or see this region's nucleotide sequence