Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3487 and GFF3488 are separated by 238 nucleotides GFF3488 and GFF3489 overlap by 41 nucleotides GFF3489 and GFF3490 are separated by 72 nucleotides GFF3490 and GFF3491 are separated by 134 nucleotides GFF3491 and GFF3492 are separated by 131 nucleotides
GFF3487 - NADP-specific glutamate dehydrogenase (EC 1.4.1.4), at 240,689 to 242,032
GFF3487
GFF3488 - FIG00638396: hypothetical protein, at 242,271 to 242,546
GFF3488
GFF3489 - 5-methyl-dCTP pyrophosphohydrolase (EC 3.6.1.-), at 242,506 to 242,922
GFF3489
GFF3490 - Exodeoxyribonuclease III (EC 3.1.11.2), at 242,995 to 243,801
GFF3490
GFF3491 - FIG01046149: hypothetical protein, at 243,936 to 244,115
GFF3491
GFF3492 - Succinylornithine transaminase (EC 2.6.1.81), at 244,247 to 245,473
GFF3492
Position (kb) 242
243
244 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 242.033 kb on - strand at 242.033 kb on - strand at 242.090 kb on + strand at 242.110 kb on - strand at 242.234 kb on + strand at 242.245 kb on + strand at 242.246 kb on - strand at 242.641 kb on + strand, within GFF3489 at 242.723 kb on + strand, within GFF3489 at 242.761 kb on + strand, within GFF3489 at 242.827 kb on + strand, within GFF3489 at 243.059 kb on + strand at 243.361 kb on + strand, within GFF3490 at 243.495 kb on + strand, within GFF3490 at 243.495 kb on + strand, within GFF3490 at 243.495 kb on + strand, within GFF3490 at 243.496 kb on - strand, within GFF3490 at 243.496 kb on - strand, within GFF3490 at 243.529 kb on - strand, within GFF3490 at 243.529 kb on - strand, within GFF3490 at 243.636 kb on - strand, within GFF3490 at 243.648 kb on - strand, within GFF3490 at 243.648 kb on - strand, within GFF3490 at 243.801 kb on + strand at 243.898 kb on + strand at 244.043 kb on + strand, within GFF3491 at 244.298 kb on + strand at 244.298 kb on + strand at 244.299 kb on - strand at 244.316 kb on - strand at 244.553 kb on - strand, within GFF3492 at 244.602 kb on - strand, within GFF3492 at 244.648 kb on + strand, within GFF3492 at 244.707 kb on - strand, within GFF3492 at 244.795 kb on + strand, within GFF3492 at 244.795 kb on + strand, within GFF3492 at 244.795 kb on + strand, within GFF3492
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 242,033 - -0.5 242,033 - -0.6 242,090 + +1.3 242,110 - +0.8 242,234 + -0.3 242,245 + +0.2 242,246 - -1.3 242,641 + GFF3489 0.32 -0.8 242,723 + GFF3489 0.52 -0.9 242,761 + GFF3489 0.61 +0.7 242,827 + GFF3489 0.77 +0.1 243,059 + -2.0 243,361 + GFF3490 0.45 -2.3 243,495 + GFF3490 0.62 -1.8 243,495 + GFF3490 0.62 -3.0 243,495 + GFF3490 0.62 -2.7 243,496 - GFF3490 0.62 -2.3 243,496 - GFF3490 0.62 -2.8 243,529 - GFF3490 0.66 -2.8 243,529 - GFF3490 0.66 -4.6 243,636 - GFF3490 0.79 -2.5 243,648 - GFF3490 0.81 -1.0 243,648 - GFF3490 0.81 -1.7 243,801 + -2.0 243,898 + -0.0 244,043 + GFF3491 0.59 -0.2 244,298 + +0.3 244,298 + -0.3 244,299 - -0.6 244,316 - -0.1 244,553 - GFF3492 0.25 -1.3 244,602 - GFF3492 0.29 -0.3 244,648 + GFF3492 0.33 -1.1 244,707 - GFF3492 0.37 +0.2 244,795 + GFF3492 0.45 -0.8 244,795 + GFF3492 0.45 -0.4 244,795 + GFF3492 0.45 -0.3
Or see this region's nucleotide sequence