Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3443 and GFF3444 overlap by 7 nucleotides GFF3444 and GFF3445 are separated by 81 nucleotides GFF3445 and GFF3446 are separated by 193 nucleotides GFF3446 and GFF3648 are separated by 8 nucleotides GFF3648 and GFF3447 are separated by 108 nucleotides
GFF3443 - Nickel transport ATP-binding protein nikD2 (TC 3.A.1.5.3), at 204,579 to 205,376
GFF3443
GFF3444 - Nickel transport ATP-binding protein nikE2 (TC 3.A.1.5.3), at 205,370 to 205,960
GFF3444
GFF3445 - Putative inner membrane protein, at 206,042 to 207,175
GFF3445
GFF3446 - Ferredoxin, at 207,369 to 207,695
GFF3446
GFF3648 - tRNA-Arg-TCT, at 207,704 to 207,780
GFF3648
GFF3447 - Candidate zinc-binding lipoprotein ZinT, at 207,889 to 208,539
GFF3447
Position (kb) 206
207
208 Strain fitness (log2 ratio) -1
0
1
2 at 205.077 kb on + strand, within GFF3443 at 205.077 kb on + strand, within GFF3443 at 205.078 kb on - strand, within GFF3443 at 205.080 kb on - strand, within GFF3443 at 205.245 kb on + strand, within GFF3443 at 205.246 kb on - strand, within GFF3443 at 205.246 kb on - strand, within GFF3443 at 205.248 kb on - strand, within GFF3443 at 205.334 kb on - strand at 205.366 kb on + strand at 205.366 kb on + strand at 205.856 kb on + strand, within GFF3444 at 205.856 kb on + strand, within GFF3444 at 205.857 kb on - strand, within GFF3444 at 205.857 kb on - strand, within GFF3444 at 205.857 kb on - strand, within GFF3444 at 205.903 kb on + strand at 205.920 kb on + strand at 206.086 kb on + strand at 206.092 kb on - strand at 206.256 kb on + strand, within GFF3445 at 206.425 kb on + strand, within GFF3445 at 206.428 kb on - strand, within GFF3445 at 206.428 kb on - strand, within GFF3445 at 206.428 kb on - strand, within GFF3445 at 206.538 kb on - strand, within GFF3445 at 206.687 kb on + strand, within GFF3445 at 206.796 kb on + strand, within GFF3445 at 206.822 kb on + strand, within GFF3445 at 206.959 kb on + strand, within GFF3445 at 206.989 kb on + strand, within GFF3445 at 206.990 kb on - strand, within GFF3445 at 207.043 kb on + strand, within GFF3445 at 207.044 kb on - strand, within GFF3445 at 207.046 kb on - strand, within GFF3445 at 207.056 kb on - strand, within GFF3445 at 207.079 kb on - strand at 207.205 kb on + strand at 207.212 kb on + strand at 207.213 kb on - strand at 207.213 kb on - strand at 207.335 kb on + strand at 207.340 kb on - strand at 207.340 kb on - strand at 207.491 kb on - strand, within GFF3446 at 207.533 kb on + strand, within GFF3446 at 207.740 kb on + strand, within GFF3648 at 208.033 kb on - strand, within GFF3447 at 208.069 kb on - strand, within GFF3447
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 205,077 + GFF3443 0.62 +0.7 205,077 + GFF3443 0.62 +0.5 205,078 - GFF3443 0.63 +0.2 205,080 - GFF3443 0.63 -0.0 205,245 + GFF3443 0.83 +0.3 205,246 - GFF3443 0.84 +0.1 205,246 - GFF3443 0.84 +0.1 205,248 - GFF3443 0.84 +0.5 205,334 - +0.4 205,366 + +0.8 205,366 + -0.9 205,856 + GFF3444 0.82 +0.1 205,856 + GFF3444 0.82 +0.8 205,857 - GFF3444 0.82 -0.4 205,857 - GFF3444 0.82 +0.1 205,857 - GFF3444 0.82 +0.4 205,903 + -0.6 205,920 + +0.2 206,086 + +0.2 206,092 - +0.3 206,256 + GFF3445 0.19 -0.6 206,425 + GFF3445 0.34 +0.1 206,428 - GFF3445 0.34 -0.7 206,428 - GFF3445 0.34 +0.0 206,428 - GFF3445 0.34 +0.8 206,538 - GFF3445 0.44 -1.3 206,687 + GFF3445 0.57 +2.5 206,796 + GFF3445 0.66 +1.6 206,822 + GFF3445 0.69 +0.8 206,959 + GFF3445 0.81 -0.2 206,989 + GFF3445 0.84 +0.1 206,990 - GFF3445 0.84 -0.1 207,043 + GFF3445 0.88 +0.3 207,044 - GFF3445 0.88 -1.1 207,046 - GFF3445 0.89 +0.3 207,056 - GFF3445 0.89 +0.3 207,079 - +0.2 207,205 + +0.4 207,212 + -0.4 207,213 - +0.7 207,213 - +0.9 207,335 + +0.6 207,340 - +0.2 207,340 - -0.1 207,491 - GFF3446 0.37 -0.5 207,533 + GFF3446 0.50 -0.1 207,740 + GFF3648 0.47 +0.4 208,033 - GFF3447 0.22 -0.3 208,069 - GFF3447 0.28 +0.6
Or see this region's nucleotide sequence