Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3387 and GFF3388 are separated by 13 nucleotides GFF3388 and GFF3389 overlap by 20 nucleotides GFF3389 and GFF3390 are separated by 10 nucleotides
GFF3387 - YcfL protein: an outer membrane lipoprotein that is part of a salvage cluster, at 152,817 to 153,191
GFF3387
GFF3388 - Lipoprotein YcfM, part of a salvage pathway of unknown substrate, at 153,205 to 153,843
GFF3388
GFF3389 - Thiamine kinase (EC 2.7.1.89) @ Adenosylcobinamide kinase (EC 2.7.1.156), at 153,824 to 154,648
GFF3389
GFF3390 - Beta N-acetyl-glucosaminidase (EC 3.2.1.52), at 154,659 to 155,684
GFF3390
Position (kb) 153
154
155 Strain fitness (log2 ratio) -1
0
1
2 at 153.048 kb on + strand, within GFF3387 at 153.133 kb on + strand, within GFF3387 at 153.183 kb on + strand at 153.264 kb on + strand at 153.433 kb on + strand, within GFF3388 at 153.433 kb on + strand, within GFF3388 at 153.507 kb on - strand, within GFF3388 at 153.529 kb on - strand, within GFF3388 at 153.576 kb on - strand, within GFF3388 at 153.688 kb on - strand, within GFF3388 at 153.729 kb on - strand, within GFF3388 at 153.734 kb on - strand, within GFF3388 at 153.771 kb on - strand, within GFF3388 at 153.782 kb on + strand at 153.783 kb on - strand at 153.807 kb on - strand at 153.842 kb on - strand at 153.949 kb on + strand, within GFF3389 at 154.069 kb on + strand, within GFF3389 at 154.089 kb on + strand, within GFF3389 at 154.155 kb on - strand, within GFF3389 at 154.155 kb on - strand, within GFF3389 at 154.155 kb on - strand, within GFF3389 at 154.217 kb on + strand, within GFF3389 at 154.346 kb on - strand, within GFF3389 at 154.347 kb on + strand, within GFF3389 at 154.347 kb on + strand, within GFF3389 at 154.348 kb on - strand, within GFF3389 at 154.348 kb on - strand, within GFF3389 at 154.350 kb on + strand, within GFF3389 at 154.524 kb on + strand, within GFF3389 at 154.568 kb on + strand at 154.633 kb on - strand at 154.637 kb on - strand at 154.745 kb on + strand at 155.062 kb on - strand, within GFF3390 at 155.237 kb on + strand, within GFF3390 at 155.238 kb on - strand, within GFF3390 at 155.285 kb on + strand, within GFF3390 at 155.343 kb on + strand, within GFF3390 at 155.439 kb on + strand, within GFF3390 at 155.480 kb on + strand, within GFF3390 at 155.573 kb on + strand, within GFF3390
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 153,048 + GFF3387 0.62 +0.2 153,133 + GFF3387 0.84 -0.1 153,183 + +0.6 153,264 + +0.4 153,433 + GFF3388 0.36 +0.5 153,433 + GFF3388 0.36 +0.4 153,507 - GFF3388 0.47 +0.3 153,529 - GFF3388 0.51 -0.6 153,576 - GFF3388 0.58 -0.4 153,688 - GFF3388 0.76 +0.1 153,729 - GFF3388 0.82 -1.3 153,734 - GFF3388 0.83 -0.7 153,771 - GFF3388 0.89 -0.0 153,782 + -0.5 153,783 - -0.8 153,807 - +0.0 153,842 - +0.8 153,949 + GFF3389 0.15 +0.3 154,069 + GFF3389 0.30 +0.5 154,089 + GFF3389 0.32 +0.8 154,155 - GFF3389 0.40 +0.1 154,155 - GFF3389 0.40 +0.4 154,155 - GFF3389 0.40 +0.2 154,217 + GFF3389 0.48 +0.3 154,346 - GFF3389 0.63 +0.0 154,347 + GFF3389 0.63 -1.6 154,347 + GFF3389 0.63 +0.2 154,348 - GFF3389 0.64 +0.1 154,348 - GFF3389 0.64 +0.0 154,350 + GFF3389 0.64 +0.5 154,524 + GFF3389 0.85 +0.2 154,568 + +0.3 154,633 - +0.7 154,637 - +0.5 154,745 + -0.1 155,062 - GFF3390 0.39 +0.2 155,237 + GFF3390 0.56 +0.7 155,238 - GFF3390 0.56 +0.3 155,285 + GFF3390 0.61 +1.2 155,343 + GFF3390 0.67 +1.8 155,439 + GFF3390 0.76 +1.1 155,480 + GFF3390 0.80 +0.2 155,573 + GFF3390 0.89 +0.5
Or see this region's nucleotide sequence