Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3342 and GFF3343 are separated by 104 nucleotides GFF3343 and GFF3344 are separated by 124 nucleotides GFF3344 and GFF3345 are separated by 63 nucleotides
GFF3342 - Dihydroorotase (EC 3.5.2.3), at 110,566 to 111,612
GFF3342
GFF3343 - Putative lipoprotein yceB precursor, at 111,717 to 112,277
GFF3343
GFF3344 - Glutaredoxin 2, at 112,402 to 113,049
GFF3344
GFF3345 - MFS superfamily export protein YceL, at 113,113 to 114,321
GFF3345
Position (kb)
111
112
113 Strain fitness (log2 ratio)
-1
0
1 at 110.926 kb on - strand, within GFF3342 at 110.926 kb on - strand, within GFF3342 at 110.951 kb on + strand, within GFF3342 at 110.982 kb on + strand, within GFF3342 at 110.982 kb on + strand, within GFF3342 at 110.982 kb on + strand, within GFF3342 at 110.983 kb on - strand, within GFF3342 at 111.016 kb on - strand, within GFF3342 at 111.049 kb on + strand, within GFF3342 at 111.049 kb on + strand, within GFF3342 at 111.050 kb on - strand, within GFF3342 at 111.054 kb on - strand, within GFF3342 at 111.163 kb on + strand, within GFF3342 at 111.164 kb on - strand, within GFF3342 at 111.166 kb on - strand, within GFF3342 at 111.209 kb on - strand, within GFF3342 at 111.306 kb on + strand, within GFF3342 at 111.517 kb on - strand at 111.518 kb on + strand at 111.519 kb on - strand at 111.797 kb on + strand, within GFF3343 at 111.841 kb on + strand, within GFF3343 at 111.861 kb on + strand, within GFF3343 at 111.862 kb on - strand, within GFF3343 at 111.943 kb on + strand, within GFF3343 at 112.065 kb on - strand, within GFF3343 at 112.068 kb on - strand, within GFF3343 at 112.108 kb on - strand, within GFF3343 at 112.204 kb on + strand, within GFF3343 at 112.204 kb on + strand, within GFF3343 at 112.231 kb on - strand at 112.231 kb on - strand at 112.288 kb on - strand at 112.306 kb on + strand at 112.461 kb on - strand at 112.684 kb on - strand, within GFF3344 at 112.750 kb on + strand, within GFF3344 at 112.763 kb on + strand, within GFF3344 at 112.848 kb on - strand, within GFF3344 at 112.848 kb on - strand, within GFF3344 at 112.957 kb on + strand, within GFF3344 at 112.965 kb on + strand, within GFF3344 at 112.966 kb on - strand, within GFF3344 at 113.034 kb on - strand at 113.037 kb on + strand at 113.039 kb on + strand at 113.039 kb on + strand at 113.086 kb on - strand at 113.096 kb on + strand at 113.096 kb on + strand at 113.106 kb on + strand at 113.158 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 110,926 - GFF3342 0.34 +0.4 110,926 - GFF3342 0.34 -0.4 110,951 + GFF3342 0.37 -1.4 110,982 + GFF3342 0.40 -0.1 110,982 + GFF3342 0.40 +0.2 110,982 + GFF3342 0.40 -0.6 110,983 - GFF3342 0.40 +0.0 111,016 - GFF3342 0.43 -0.4 111,049 + GFF3342 0.46 +0.1 111,049 + GFF3342 0.46 -0.1 111,050 - GFF3342 0.46 +0.4 111,054 - GFF3342 0.47 +0.2 111,163 + GFF3342 0.57 -0.4 111,164 - GFF3342 0.57 -0.4 111,166 - GFF3342 0.57 -0.9 111,209 - GFF3342 0.61 +0.1 111,306 + GFF3342 0.71 -0.1 111,517 - +0.8 111,518 + +0.1 111,519 - -1.2 111,797 + GFF3343 0.14 -1.1 111,841 + GFF3343 0.22 +0.1 111,861 + GFF3343 0.26 +0.2 111,862 - GFF3343 0.26 +0.5 111,943 + GFF3343 0.40 +0.6 112,065 - GFF3343 0.62 +0.4 112,068 - GFF3343 0.63 -0.9 112,108 - GFF3343 0.70 +1.2 112,204 + GFF3343 0.87 +0.3 112,204 + GFF3343 0.87 +1.4 112,231 - +0.3 112,231 - -1.2 112,288 - -0.2 112,306 + +0.3 112,461 - +0.9 112,684 - GFF3344 0.44 +0.3 112,750 + GFF3344 0.54 -0.9 112,763 + GFF3344 0.56 -0.5 112,848 - GFF3344 0.69 +0.1 112,848 - GFF3344 0.69 +0.2 112,957 + GFF3344 0.86 +0.7 112,965 + GFF3344 0.87 -1.0 112,966 - GFF3344 0.87 -0.7 113,034 - +0.8 113,037 + -0.0 113,039 + +0.3 113,039 + -0.5 113,086 - +0.4 113,096 + +0.4 113,096 + -0.6 113,106 + +0.3 113,158 - -0.4
Or see this region's nucleotide sequence