Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3291 and GFF3292 overlap by 4 nucleotides GFF3292 and GFF3293 overlap by 11 nucleotides GFF3293 and GFF3294 are separated by 132 nucleotides
GFF3291 - Membrane protein, suppressor for copper-sensitivity ScsB, at 62,431 to 64,317
GFF3291
GFF3292 - Secreted protein, suppressor for copper-sensitivity ScsC, at 64,314 to 64,937
GFF3292
GFF3293 - Membrane protein, suppressor for copper-sensitivity ScsD, at 64,927 to 65,433
GFF3293
GFF3294 - Glucose-1-phosphatase (EC 3.1.3.10), at 65,566 to 66,807
GFF3294
Position (kb) 64
65 Strain fitness (log2 ratio) -1
0
1
2 at 63.321 kb on - strand, within GFF3291 at 63.451 kb on - strand, within GFF3291 at 63.510 kb on - strand, within GFF3291 at 63.594 kb on + strand, within GFF3291 at 63.594 kb on + strand, within GFF3291 at 63.595 kb on - strand, within GFF3291 at 63.616 kb on + strand, within GFF3291 at 63.895 kb on - strand, within GFF3291 at 64.054 kb on - strand, within GFF3291 at 64.072 kb on + strand, within GFF3291 at 64.217 kb on + strand at 64.217 kb on + strand at 64.217 kb on + strand at 64.218 kb on - strand at 64.218 kb on - strand at 64.218 kb on - strand at 64.503 kb on + strand, within GFF3292 at 64.581 kb on - strand, within GFF3292 at 64.599 kb on - strand, within GFF3292 at 64.705 kb on - strand, within GFF3292 at 64.721 kb on + strand, within GFF3292 at 65.054 kb on + strand, within GFF3293 at 65.055 kb on - strand, within GFF3293 at 65.058 kb on + strand, within GFF3293 at 65.059 kb on - strand, within GFF3293 at 65.154 kb on + strand, within GFF3293 at 65.157 kb on + strand, within GFF3293 at 65.158 kb on - strand, within GFF3293 at 65.390 kb on - strand at 65.472 kb on + strand at 65.561 kb on + strand at 65.562 kb on - strand at 65.658 kb on - strand at 65.658 kb on - strand at 65.806 kb on + strand, within GFF3294 at 65.885 kb on + strand, within GFF3294 at 65.885 kb on + strand, within GFF3294 at 65.886 kb on - strand, within GFF3294 at 65.886 kb on - strand, within GFF3294 at 65.886 kb on - strand, within GFF3294 at 65.887 kb on + strand, within GFF3294 at 65.887 kb on + strand, within GFF3294 at 65.888 kb on - strand, within GFF3294 at 65.888 kb on - strand, within GFF3294
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 63,321 - GFF3291 0.47 -1.1 63,451 - GFF3291 0.54 -0.3 63,510 - GFF3291 0.57 +0.2 63,594 + GFF3291 0.62 +0.6 63,594 + GFF3291 0.62 +0.7 63,595 - GFF3291 0.62 +2.0 63,616 + GFF3291 0.63 -0.4 63,895 - GFF3291 0.78 -0.2 64,054 - GFF3291 0.86 -0.3 64,072 + GFF3291 0.87 +0.3 64,217 + +0.3 64,217 + +0.0 64,217 + +0.2 64,218 - +0.2 64,218 - +0.1 64,218 - +0.1 64,503 + GFF3292 0.30 -0.6 64,581 - GFF3292 0.43 -0.7 64,599 - GFF3292 0.46 -0.1 64,705 - GFF3292 0.63 -1.1 64,721 + GFF3292 0.65 +0.4 65,054 + GFF3293 0.25 -0.5 65,055 - GFF3293 0.25 +0.2 65,058 + GFF3293 0.26 -0.0 65,059 - GFF3293 0.26 -0.9 65,154 + GFF3293 0.45 -0.6 65,157 + GFF3293 0.45 +0.2 65,158 - GFF3293 0.46 +0.0 65,390 - +0.1 65,472 + -0.2 65,561 + +0.2 65,562 - +0.4 65,658 - +0.4 65,658 - -0.3 65,806 + GFF3294 0.19 +1.1 65,885 + GFF3294 0.26 -0.8 65,885 + GFF3294 0.26 +0.0 65,886 - GFF3294 0.26 -1.3 65,886 - GFF3294 0.26 +0.3 65,886 - GFF3294 0.26 -0.9 65,887 + GFF3294 0.26 -0.5 65,887 + GFF3294 0.26 +0.2 65,888 - GFF3294 0.26 -0.0 65,888 - GFF3294 0.26 +0.6
Or see this region's nucleotide sequence