Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3282 and GFF3283 are separated by 10 nucleotides GFF3283 and GFF3284 are separated by 71 nucleotides GFF3284 and GFF3285 are separated by 9 nucleotides GFF3285 and GFF3286 are separated by 13 nucleotides
GFF3282 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 54,225 to 55,028
GFF3282
GFF3283 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.-), at 55,039 to 55,830
GFF3283
GFF3284 - 4-hydroxyphenylacetate symporter, major facilitator superfamily (MFS), at 55,902 to 57,278
GFF3284
GFF3285 - Transcriptional activator of 4-hydroxyphenylacetate 3-monooxygenase operon, XylS/AraC family, at 57,288 to 58,184
GFF3285
GFF3286 - Ribonuclease Z (EC 3.1.26.11), at 58,198 to 59,136
GFF3286
Position (kb) 55
56
57
58 Strain fitness (log2 ratio) -1
0
1 at 55.130 kb on + strand, within GFF3283 at 55.313 kb on + strand, within GFF3283 at 55.381 kb on - strand, within GFF3283 at 55.469 kb on + strand, within GFF3283 at 55.548 kb on + strand, within GFF3283 at 55.643 kb on + strand, within GFF3283 at 55.702 kb on + strand, within GFF3283 at 55.870 kb on + strand at 55.876 kb on - strand at 55.992 kb on - strand at 56.034 kb on - strand at 56.149 kb on - strand, within GFF3284 at 56.149 kb on - strand, within GFF3284 at 56.149 kb on - strand, within GFF3284 at 56.149 kb on - strand, within GFF3284 at 56.243 kb on + strand, within GFF3284 at 56.332 kb on + strand, within GFF3284 at 56.444 kb on + strand, within GFF3284 at 56.445 kb on - strand, within GFF3284 at 56.445 kb on - strand, within GFF3284 at 56.465 kb on - strand, within GFF3284 at 56.531 kb on + strand, within GFF3284 at 56.713 kb on + strand, within GFF3284 at 56.714 kb on - strand, within GFF3284 at 56.721 kb on + strand, within GFF3284 at 56.722 kb on - strand, within GFF3284 at 56.722 kb on - strand, within GFF3284 at 56.723 kb on + strand, within GFF3284 at 56.750 kb on + strand, within GFF3284 at 56.768 kb on + strand, within GFF3284 at 57.287 kb on + strand at 57.287 kb on + strand at 57.287 kb on + strand at 57.287 kb on + strand at 57.288 kb on - strand at 57.317 kb on + strand at 57.545 kb on + strand, within GFF3285 at 57.791 kb on + strand, within GFF3285 at 57.881 kb on - strand, within GFF3285 at 57.881 kb on - strand, within GFF3285 at 58.218 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 55,130 + GFF3283 0.11 -1.0 55,313 + GFF3283 0.35 +1.0 55,381 - GFF3283 0.43 -0.9 55,469 + GFF3283 0.54 +0.3 55,548 + GFF3283 0.64 +0.5 55,643 + GFF3283 0.76 +0.5 55,702 + GFF3283 0.84 -1.6 55,870 + +0.7 55,876 - +0.2 55,992 - +0.2 56,034 - -1.0 56,149 - GFF3284 0.18 +1.6 56,149 - GFF3284 0.18 -1.2 56,149 - GFF3284 0.18 +0.9 56,149 - GFF3284 0.18 -0.1 56,243 + GFF3284 0.25 -0.1 56,332 + GFF3284 0.31 +0.6 56,444 + GFF3284 0.39 +0.4 56,445 - GFF3284 0.39 +0.4 56,445 - GFF3284 0.39 +0.6 56,465 - GFF3284 0.41 -0.1 56,531 + GFF3284 0.46 +0.2 56,713 + GFF3284 0.59 +0.9 56,714 - GFF3284 0.59 +0.5 56,721 + GFF3284 0.59 -0.3 56,722 - GFF3284 0.60 +0.3 56,722 - GFF3284 0.60 -0.3 56,723 + GFF3284 0.60 +0.1 56,750 + GFF3284 0.62 -0.6 56,768 + GFF3284 0.63 -0.4 57,287 + +0.0 57,287 + +0.5 57,287 + -0.2 57,287 + +0.4 57,288 - +0.2 57,317 + -0.2 57,545 + GFF3285 0.29 -0.1 57,791 + GFF3285 0.56 -0.2 57,881 - GFF3285 0.66 -1.1 57,881 - GFF3285 0.66 +0.1 58,218 + +0.8
Or see this region's nucleotide sequence