Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF326 and GFF327 overlap by 4 nucleotides GFF327 and GFF328 are separated by 3 nucleotides GFF328 and GFF329 overlap by 14 nucleotides GFF329 and GFF330 are separated by 235 nucleotides
GFF326 - PTS system, mannose-specific IIC component, at 41,815 to 42,564
GFF326
GFF327 - PTS system, mannose-specific IID component (EC 2.7.1.69), at 42,561 to 43,421
GFF327
GFF328 - L-seryl-tRNA(Sec) selenium transferase-related protein, at 43,425 to 44,534
GFF328
GFF329 - 4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16), at 44,521 to 45,264
GFF329
GFF330 - Transposase, at 45,500 to 46,441
GFF330
Position (kb)
43
44
45 Strain fitness (log2 ratio)
-1
0
1 at 42.577 kb on + strand at 42.577 kb on + strand at 42.578 kb on - strand at 42.578 kb on - strand at 42.654 kb on - strand, within GFF327 at 42.688 kb on - strand, within GFF327 at 42.697 kb on + strand, within GFF327 at 42.791 kb on + strand, within GFF327 at 42.800 kb on + strand, within GFF327 at 42.842 kb on + strand, within GFF327 at 43.072 kb on - strand, within GFF327 at 43.094 kb on + strand, within GFF327 at 43.094 kb on + strand, within GFF327 at 43.094 kb on + strand, within GFF327 at 43.094 kb on + strand, within GFF327 at 43.095 kb on - strand, within GFF327 at 43.095 kb on - strand, within GFF327 at 43.095 kb on - strand, within GFF327 at 43.206 kb on + strand, within GFF327 at 43.207 kb on - strand, within GFF327 at 43.326 kb on + strand, within GFF327 at 43.332 kb on + strand, within GFF327 at 43.394 kb on - strand at 43.419 kb on + strand at 43.419 kb on + strand at 43.420 kb on - strand at 43.437 kb on - strand at 43.437 kb on - strand at 43.607 kb on + strand, within GFF328 at 43.622 kb on + strand, within GFF328 at 43.623 kb on - strand, within GFF328 at 43.623 kb on - strand, within GFF328 at 43.623 kb on - strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.627 kb on - strand, within GFF328 at 43.627 kb on - strand, within GFF328 at 43.627 kb on - strand, within GFF328 at 43.627 kb on - strand, within GFF328 at 43.742 kb on - strand, within GFF328 at 43.760 kb on + strand, within GFF328 at 44.045 kb on + strand, within GFF328 at 44.049 kb on + strand, within GFF328 at 44.050 kb on - strand, within GFF328 at 44.050 kb on - strand, within GFF328 at 44.070 kb on - strand, within GFF328 at 44.099 kb on + strand, within GFF328 at 44.099 kb on + strand, within GFF328 at 44.099 kb on + strand, within GFF328 at 44.190 kb on + strand, within GFF328 at 44.191 kb on - strand, within GFF328 at 44.600 kb on - strand, within GFF329 at 44.600 kb on - strand, within GFF329 at 44.600 kb on - strand, within GFF329 at 44.600 kb on - strand, within GFF329 at 44.600 kb on - strand, within GFF329 at 44.657 kb on + strand, within GFF329 at 44.657 kb on + strand, within GFF329 at 44.743 kb on + strand, within GFF329 at 44.743 kb on + strand, within GFF329 at 44.746 kb on + strand, within GFF329 at 44.773 kb on - strand, within GFF329 at 45.041 kb on + strand, within GFF329 at 45.041 kb on + strand, within GFF329 at 45.041 kb on + strand, within GFF329 at 45.042 kb on - strand, within GFF329 at 45.245 kb on + strand at 45.246 kb on - strand at 45.313 kb on + strand at 45.314 kb on - strand at 45.314 kb on - strand at 45.382 kb on - strand at 45.382 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 42,577 + +0.2 42,577 + +0.1 42,578 - -0.3 42,578 - -0.6 42,654 - GFF327 0.11 -0.4 42,688 - GFF327 0.15 +0.0 42,697 + GFF327 0.16 +0.1 42,791 + GFF327 0.27 +0.0 42,800 + GFF327 0.28 -0.4 42,842 + GFF327 0.33 +0.3 43,072 - GFF327 0.59 +0.5 43,094 + GFF327 0.62 -0.2 43,094 + GFF327 0.62 -1.2 43,094 + GFF327 0.62 +0.1 43,094 + GFF327 0.62 +0.3 43,095 - GFF327 0.62 +0.3 43,095 - GFF327 0.62 +0.7 43,095 - GFF327 0.62 -0.5 43,206 + GFF327 0.75 -0.8 43,207 - GFF327 0.75 -0.0 43,326 + GFF327 0.89 -0.8 43,332 + GFF327 0.90 +0.6 43,394 - +0.0 43,419 + -0.1 43,419 + -0.1 43,420 - -0.9 43,437 - +0.7 43,437 - +1.2 43,607 + GFF328 0.16 +0.2 43,622 + GFF328 0.18 +0.4 43,623 - GFF328 0.18 +0.5 43,623 - GFF328 0.18 +0.3 43,623 - GFF328 0.18 -0.9 43,626 + GFF328 0.18 -0.1 43,626 + GFF328 0.18 +0.7 43,626 + GFF328 0.18 -0.3 43,626 + GFF328 0.18 +1.6 43,626 + GFF328 0.18 -0.2 43,626 + GFF328 0.18 +0.2 43,626 + GFF328 0.18 +0.4 43,626 + GFF328 0.18 +0.4 43,627 - GFF328 0.18 -0.6 43,627 - GFF328 0.18 +0.5 43,627 - GFF328 0.18 -0.2 43,627 - GFF328 0.18 +0.0 43,742 - GFF328 0.29 +0.1 43,760 + GFF328 0.30 +0.5 44,045 + GFF328 0.56 -0.4 44,049 + GFF328 0.56 -0.2 44,050 - GFF328 0.56 +0.6 44,050 - GFF328 0.56 +0.5 44,070 - GFF328 0.58 -0.3 44,099 + GFF328 0.61 +0.1 44,099 + GFF328 0.61 +0.5 44,099 + GFF328 0.61 -0.0 44,190 + GFF328 0.69 -0.0 44,191 - GFF328 0.69 +0.8 44,600 - GFF329 0.11 -0.3 44,600 - GFF329 0.11 -0.6 44,600 - GFF329 0.11 -0.4 44,600 - GFF329 0.11 -0.1 44,600 - GFF329 0.11 -0.1 44,657 + GFF329 0.18 -0.1 44,657 + GFF329 0.18 +0.2 44,743 + GFF329 0.30 -0.5 44,743 + GFF329 0.30 +0.5 44,746 + GFF329 0.30 -0.6 44,773 - GFF329 0.34 +0.4 45,041 + GFF329 0.70 +0.1 45,041 + GFF329 0.70 -0.4 45,041 + GFF329 0.70 -0.2 45,042 - GFF329 0.70 -0.2 45,245 + +0.2 45,246 - -0.1 45,313 + -0.6 45,314 - -1.2 45,314 - -1.2 45,382 - +0.3 45,382 - -0.3
Or see this region's nucleotide sequence