Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3253 and GFF3254 are separated by 35 nucleotides GFF3254 and GFF3255 are separated by 94 nucleotides GFF3255 and GFF3256 are separated by 170 nucleotides
GFF3253 - DNA helicase IV, at 28,300 to 30,354
GFF3253
GFF3254 - Methylglyoxal synthase (EC 4.2.3.3), at 30,390 to 30,848
GFF3254
GFF3255 - UPF0319 protein YccT precursor, at 30,943 to 31,605
GFF3255
GFF3256 - Succinyl-CoA synthetase, alpha subunit-related enzymes, at 31,776 to 32,192
GFF3256
Position (kb)
30
31 Strain fitness (log2 ratio)
-1
0
1 at 29.680 kb on - strand, within GFF3253 at 29.884 kb on - strand, within GFF3253 at 29.985 kb on + strand, within GFF3253 at 30.030 kb on - strand, within GFF3253 at 30.236 kb on + strand at 30.237 kb on - strand at 30.239 kb on - strand at 30.392 kb on - strand at 30.442 kb on - strand, within GFF3254 at 30.473 kb on + strand, within GFF3254 at 30.677 kb on + strand, within GFF3254 at 30.721 kb on + strand, within GFF3254 at 30.866 kb on - strand at 31.155 kb on - strand, within GFF3255 at 31.200 kb on + strand, within GFF3255 at 31.528 kb on - strand, within GFF3255 at 31.605 kb on + strand at 31.658 kb on + strand at 31.677 kb on + strand at 31.677 kb on + strand at 31.679 kb on + strand at 31.679 kb on + strand at 31.685 kb on + strand at 31.685 kb on + strand at 31.686 kb on - strand at 31.706 kb on + strand at 31.706 kb on + strand at 31.732 kb on + strand at 31.823 kb on + strand, within GFF3256 at 31.835 kb on - strand, within GFF3256
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 29,680 - GFF3253 0.67 +0.5 29,884 - GFF3253 0.77 +0.1 29,985 + GFF3253 0.82 +0.0 30,030 - GFF3253 0.84 -0.0 30,236 + -0.3 30,237 - -0.5 30,239 - -0.4 30,392 - -1.1 30,442 - GFF3254 0.11 +0.3 30,473 + GFF3254 0.18 +0.3 30,677 + GFF3254 0.63 +0.1 30,721 + GFF3254 0.72 +0.3 30,866 - -1.2 31,155 - GFF3255 0.32 -0.3 31,200 + GFF3255 0.39 +0.5 31,528 - GFF3255 0.88 +0.3 31,605 + +0.4 31,658 + +0.7 31,677 + -0.9 31,677 + +0.4 31,679 + +0.8 31,679 + +0.3 31,685 + +0.9 31,685 + +0.6 31,686 - -0.8 31,706 + +0.4 31,706 + +0.6 31,732 + +0.6 31,823 + GFF3256 0.11 +0.1 31,835 - GFF3256 0.14 -0.4
Or see this region's nucleotide sequence