Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2982 and GFF2983 are separated by 18 nucleotides GFF2983 and GFF3213 are separated by 131 nucleotides GFF3213 and GFF2984 are separated by 79 nucleotides GFF2984 and GFF2985 are separated by 85 nucleotides
GFF2982 - hypothetical protein, at 1,489 to 1,605
GFF2982
GFF2983 - probable lipoprotein YPO2292, at 1,624 to 2,424
GFF2983
GFF3213 - tRNA-Gly-CCC, at 2,556 to 2,629
GFF3213
GFF2984 - Uncharacterized lipoprotein YgeR precursor, at 2,709 to 3,461
GFF2984
GFF2985 - hypothetical protein, at 3,547 to 3,681
GFF2985
Position (kb)
2
3 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1.623 kb on - strand at 1.623 kb on - strand at 1.768 kb on + strand, within GFF2983 at 1.921 kb on + strand, within GFF2983 at 1.922 kb on - strand, within GFF2983 at 1.969 kb on - strand, within GFF2983 at 1.969 kb on - strand, within GFF2983 at 2.072 kb on + strand, within GFF2983 at 2.173 kb on - strand, within GFF2983 at 2.239 kb on + strand, within GFF2983 at 2.239 kb on + strand, within GFF2983 at 2.239 kb on + strand, within GFF2983 at 2.295 kb on - strand, within GFF2983 at 2.470 kb on - strand at 2.470 kb on - strand at 2.585 kb on + strand, within GFF3213 at 2.588 kb on - strand, within GFF3213 at 2.699 kb on + strand at 2.791 kb on - strand, within GFF2984 at 2.791 kb on - strand, within GFF2984 at 2.883 kb on - strand, within GFF2984 at 2.897 kb on + strand, within GFF2984 at 2.898 kb on - strand, within GFF2984 at 2.902 kb on + strand, within GFF2984 at 2.918 kb on + strand, within GFF2984 at 3.069 kb on - strand, within GFF2984 at 3.084 kb on + strand, within GFF2984 at 3.242 kb on + strand, within GFF2984 at 3.243 kb on - strand, within GFF2984 at 3.246 kb on - strand, within GFF2984 at 3.247 kb on + strand, within GFF2984 at 3.248 kb on - strand, within GFF2984 at 3.312 kb on - strand, within GFF2984 at 3.341 kb on + strand, within GFF2984 at 3.343 kb on + strand, within GFF2984 at 3.426 kb on - strand at 3.497 kb on + strand at 3.567 kb on + strand, within GFF2985 at 3.584 kb on + strand, within GFF2985 at 3.585 kb on - strand, within GFF2985 at 3.585 kb on - strand, within GFF2985 at 3.588 kb on - strand, within GFF2985
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 1,623 - +0.0 1,623 - +0.7 1,768 + GFF2983 0.18 +0.3 1,921 + GFF2983 0.37 -0.1 1,922 - GFF2983 0.37 +0.3 1,969 - GFF2983 0.43 +0.0 1,969 - GFF2983 0.43 +0.5 2,072 + GFF2983 0.56 +0.2 2,173 - GFF2983 0.69 +0.4 2,239 + GFF2983 0.77 +0.6 2,239 + GFF2983 0.77 +0.4 2,239 + GFF2983 0.77 +0.8 2,295 - GFF2983 0.84 +0.0 2,470 - -1.0 2,470 - -0.7 2,585 + GFF3213 0.39 +0.6 2,588 - GFF3213 0.43 +0.4 2,699 + +0.4 2,791 - GFF2984 0.11 +0.6 2,791 - GFF2984 0.11 -0.4 2,883 - GFF2984 0.23 +3.5 2,897 + GFF2984 0.25 +0.3 2,898 - GFF2984 0.25 -0.3 2,902 + GFF2984 0.26 +0.3 2,918 + GFF2984 0.28 -0.0 3,069 - GFF2984 0.48 +0.3 3,084 + GFF2984 0.50 -0.4 3,242 + GFF2984 0.71 -0.6 3,243 - GFF2984 0.71 +0.1 3,246 - GFF2984 0.71 -0.3 3,247 + GFF2984 0.71 +0.3 3,248 - GFF2984 0.72 +0.1 3,312 - GFF2984 0.80 -0.2 3,341 + GFF2984 0.84 -1.1 3,343 + GFF2984 0.84 +0.1 3,426 - -1.0 3,497 + +0.3 3,567 + GFF2985 0.15 +1.0 3,584 + GFF2985 0.27 +0.1 3,585 - GFF2985 0.28 +0.2 3,585 - GFF2985 0.28 +0.0 3,588 - GFF2985 0.30 +0.5
Or see this region's nucleotide sequence