Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF315 and GFF316 are separated by 90 nucleotides GFF316 and GFF317 overlap by 17 nucleotides GFF317 and GFF318 are separated by 170 nucleotides GFF318 and GFF319 are separated by 59 nucleotides
GFF315 - Phosphotransferase system HPr enzyme STM3779, at 33,258 to 33,527
GFF315
GFF316 - Putative DNA-binding protein in cluster with Type I restriction-modification system, at 33,618 to 33,941
GFF316
GFF317 - FIG074102: hypothetical protein, at 33,925 to 34,329
GFF317
GFF318 - Putative transport protein, at 34,500 to 35,693
GFF318
GFF319 - Beta-glucosidase (EC 3.2.1.21), at 35,753 to 37,135
GFF319
Position (kb) 34
35
36 Strain fitness (log2 ratio) -1
0
1 at 33.524 kb on - strand at 33.634 kb on - strand at 33.656 kb on + strand, within GFF316 at 33.656 kb on + strand, within GFF316 at 33.657 kb on - strand, within GFF316 at 33.657 kb on - strand, within GFF316 at 33.657 kb on - strand, within GFF316 at 33.966 kb on + strand, within GFF317 at 33.967 kb on - strand, within GFF317 at 34.007 kb on + strand, within GFF317 at 34.018 kb on + strand, within GFF317 at 34.046 kb on + strand, within GFF317 at 34.050 kb on + strand, within GFF317 at 34.050 kb on + strand, within GFF317 at 34.050 kb on + strand, within GFF317 at 34.051 kb on - strand, within GFF317 at 34.051 kb on - strand, within GFF317 at 34.051 kb on - strand, within GFF317 at 34.176 kb on + strand, within GFF317 at 34.216 kb on + strand, within GFF317 at 34.216 kb on + strand, within GFF317 at 34.350 kb on + strand at 34.456 kb on - strand at 34.456 kb on - strand at 34.456 kb on - strand at 34.599 kb on - strand at 34.733 kb on + strand, within GFF318 at 34.756 kb on - strand, within GFF318 at 34.756 kb on - strand, within GFF318 at 34.757 kb on + strand, within GFF318 at 34.763 kb on + strand, within GFF318 at 34.831 kb on + strand, within GFF318 at 34.832 kb on - strand, within GFF318 at 34.832 kb on - strand, within GFF318 at 35.021 kb on + strand, within GFF318 at 35.053 kb on + strand, within GFF318 at 35.054 kb on - strand, within GFF318 at 35.123 kb on + strand, within GFF318 at 35.130 kb on - strand, within GFF318 at 35.178 kb on - strand, within GFF318 at 35.178 kb on - strand, within GFF318 at 35.211 kb on + strand, within GFF318 at 35.228 kb on + strand, within GFF318 at 35.286 kb on - strand, within GFF318 at 35.558 kb on + strand, within GFF318 at 35.558 kb on + strand, within GFF318 at 35.746 kb on + strand at 35.862 kb on + strand at 36.103 kb on - strand, within GFF319 at 36.249 kb on + strand, within GFF319 at 36.249 kb on + strand, within GFF319 at 36.249 kb on + strand, within GFF319 at 36.250 kb on - strand, within GFF319 at 36.372 kb on - strand, within GFF319 at 36.476 kb on + strand, within GFF319 at 36.479 kb on + strand, within GFF319 at 36.480 kb on - strand, within GFF319 at 36.480 kb on - strand, within GFF319 at 36.682 kb on + strand, within GFF319 at 36.683 kb on - strand, within GFF319 at 36.683 kb on - strand, within GFF319
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 33,524 - +0.1 33,634 - +0.5 33,656 + GFF316 0.12 +0.9 33,656 + GFF316 0.12 +0.1 33,657 - GFF316 0.12 +0.1 33,657 - GFF316 0.12 +0.2 33,657 - GFF316 0.12 +0.2 33,966 + GFF317 0.10 +0.0 33,967 - GFF317 0.10 +0.7 34,007 + GFF317 0.20 -0.7 34,018 + GFF317 0.23 -0.2 34,046 + GFF317 0.30 +0.3 34,050 + GFF317 0.31 +0.3 34,050 + GFF317 0.31 -0.2 34,050 + GFF317 0.31 +0.1 34,051 - GFF317 0.31 +0.1 34,051 - GFF317 0.31 +0.1 34,051 - GFF317 0.31 +0.1 34,176 + GFF317 0.62 +0.1 34,216 + GFF317 0.72 -0.2 34,216 + GFF317 0.72 -0.9 34,350 + -1.3 34,456 - -1.0 34,456 - -0.0 34,456 - +1.5 34,599 - +0.3 34,733 + GFF318 0.20 -0.2 34,756 - GFF318 0.21 -0.6 34,756 - GFF318 0.21 +0.4 34,757 + GFF318 0.22 -0.2 34,763 + GFF318 0.22 +0.0 34,831 + GFF318 0.28 -0.3 34,832 - GFF318 0.28 +0.0 34,832 - GFF318 0.28 -0.9 35,021 + GFF318 0.44 +0.2 35,053 + GFF318 0.46 -0.4 35,054 - GFF318 0.46 +0.3 35,123 + GFF318 0.52 +0.1 35,130 - GFF318 0.53 -1.3 35,178 - GFF318 0.57 +0.1 35,178 - GFF318 0.57 -1.2 35,211 + GFF318 0.60 +0.1 35,228 + GFF318 0.61 +0.3 35,286 - GFF318 0.66 -0.2 35,558 + GFF318 0.89 +0.8 35,558 + GFF318 0.89 -0.9 35,746 + +0.0 35,862 + +0.2 36,103 - GFF319 0.25 +0.3 36,249 + GFF319 0.36 +0.0 36,249 + GFF319 0.36 +0.0 36,249 + GFF319 0.36 -0.0 36,250 - GFF319 0.36 -0.6 36,372 - GFF319 0.45 +0.1 36,476 + GFF319 0.52 +0.1 36,479 + GFF319 0.52 -1.1 36,480 - GFF319 0.53 -0.1 36,480 - GFF319 0.53 +0.2 36,682 + GFF319 0.67 +0.9 36,683 - GFF319 0.67 +0.2 36,683 - GFF319 0.67 +0.0
Or see this region's nucleotide sequence