Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3170 and GFF3171 are separated by 104 nucleotides GFF3171 and GFF3172 are separated by 97 nucleotides GFF3172 and GFF3173 are separated by 106 nucleotides
GFF3170 - tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25), at 174,356 to 175,597
GFF3170
GFF3171 - Undecaprenyl-diphosphatase (EC 3.6.1.27), at 175,702 to 176,523
GFF3171
GFF3172 - Dihydroneopterin aldolase (EC 4.1.2.25), at 176,621 to 176,980
GFF3172
GFF3173 - Acyl-phosphate:glycerol-3-phosphate O-acyltransferase PlsY, at 177,087 to 177,698
GFF3173
Position (kb) 175
176
177 Strain fitness (log2 ratio) -2
-1
0
1
2 at 175.776 kb on + strand at 176.154 kb on - strand, within GFF3171 at 176.173 kb on - strand, within GFF3171 at 176.176 kb on + strand, within GFF3171 at 176.177 kb on - strand, within GFF3171 at 176.265 kb on + strand, within GFF3171 at 176.266 kb on - strand, within GFF3171 at 176.266 kb on - strand, within GFF3171 at 176.355 kb on + strand, within GFF3171 at 176.356 kb on - strand, within GFF3171 at 176.427 kb on + strand, within GFF3171 at 176.528 kb on - strand at 176.571 kb on - strand at 176.585 kb on - strand at 177.050 kb on + strand at 177.051 kb on - strand at 177.086 kb on + strand at 177.223 kb on + strand, within GFF3173 at 177.279 kb on - strand, within GFF3173 at 177.284 kb on + strand, within GFF3173 at 177.285 kb on - strand, within GFF3173 at 177.285 kb on - strand, within GFF3173 at 177.307 kb on - strand, within GFF3173 at 177.393 kb on - strand, within GFF3173 at 177.511 kb on + strand, within GFF3173
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 175,776 + +2.1 176,154 - GFF3171 0.55 +0.3 176,173 - GFF3171 0.57 -1.0 176,176 + GFF3171 0.58 +0.0 176,177 - GFF3171 0.58 -1.4 176,265 + GFF3171 0.68 -1.2 176,266 - GFF3171 0.69 -0.1 176,266 - GFF3171 0.69 -0.3 176,355 + GFF3171 0.79 -2.2 176,356 - GFF3171 0.80 -0.5 176,427 + GFF3171 0.88 -1.4 176,528 - -1.4 176,571 - +0.7 176,585 - +0.4 177,050 + -1.3 177,051 - +0.2 177,086 + -0.6 177,223 + GFF3173 0.22 +0.0 177,279 - GFF3173 0.31 -0.2 177,284 + GFF3173 0.32 -0.2 177,285 - GFF3173 0.32 +0.4 177,285 - GFF3173 0.32 -0.1 177,307 - GFF3173 0.36 -0.0 177,393 - GFF3173 0.50 +0.1 177,511 + GFF3173 0.69 -0.6
Or see this region's nucleotide sequence