Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3156 and GFF3157 are separated by 35 nucleotides GFF3157 and GFF3158 are separated by 19 nucleotides GFF3158 and GFF3159 are separated by 14 nucleotides
GFF3156 - Putative arylsulfate sulfotransferase (EC 2.8.2.22), at 159,637 to 160,602
GFF3156
GFF3157 - Putative arylsulfate sulfotransferase (EC 2.8.2.22), at 160,638 to 161,432
GFF3157
GFF3158 - Periplasmic thiol:disulfide interchange protein, DsbA-like, at 161,452 to 162,123
GFF3158
GFF3159 - Inner membrane thiol:disulfide oxidoreductase, DsbB-like, at 162,138 to 162,815
GFF3159
Position (kb)
160
161
162 Strain fitness (log2 ratio)
-1
0
1 at 159.845 kb on + strand, within GFF3156 at 159.881 kb on - strand, within GFF3156 at 159.919 kb on - strand, within GFF3156 at 160.003 kb on + strand, within GFF3156 at 160.038 kb on + strand, within GFF3156 at 160.297 kb on - strand, within GFF3156 at 160.546 kb on + strand at 160.547 kb on - strand at 160.556 kb on + strand at 160.557 kb on - strand at 160.560 kb on - strand at 160.586 kb on + strand at 160.678 kb on + strand at 160.773 kb on - strand, within GFF3157 at 160.800 kb on + strand, within GFF3157 at 160.801 kb on - strand, within GFF3157 at 160.898 kb on + strand, within GFF3157 at 160.911 kb on - strand, within GFF3157 at 160.955 kb on + strand, within GFF3157 at 161.135 kb on + strand, within GFF3157 at 161.197 kb on - strand, within GFF3157 at 161.208 kb on - strand, within GFF3157 at 161.214 kb on - strand, within GFF3157 at 161.217 kb on - strand, within GFF3157 at 161.274 kb on - strand, within GFF3157 at 161.477 kb on + strand at 161.586 kb on + strand, within GFF3158 at 162.047 kb on - strand, within GFF3158 at 162.047 kb on - strand, within GFF3158 at 162.051 kb on + strand, within GFF3158 at 162.052 kb on - strand, within GFF3158 at 162.052 kb on - strand, within GFF3158 at 162.056 kb on - strand at 162.285 kb on + strand, within GFF3159 at 162.318 kb on + strand, within GFF3159 at 162.347 kb on - strand, within GFF3159 at 162.370 kb on + strand, within GFF3159
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 159,845 + GFF3156 0.22 +0.3 159,881 - GFF3156 0.25 -0.1 159,919 - GFF3156 0.29 -0.2 160,003 + GFF3156 0.38 -0.0 160,038 + GFF3156 0.42 -0.5 160,297 - GFF3156 0.68 -0.3 160,546 + +0.3 160,547 - +1.0 160,556 + +0.1 160,557 - +0.1 160,560 - +0.2 160,586 + -0.6 160,678 + +0.9 160,773 - GFF3157 0.17 +0.1 160,800 + GFF3157 0.20 -0.2 160,801 - GFF3157 0.21 -0.2 160,898 + GFF3157 0.33 -0.4 160,911 - GFF3157 0.34 -1.3 160,955 + GFF3157 0.40 +0.5 161,135 + GFF3157 0.63 -0.6 161,197 - GFF3157 0.70 -0.7 161,208 - GFF3157 0.72 -1.2 161,214 - GFF3157 0.72 -1.1 161,217 - GFF3157 0.73 -1.7 161,274 - GFF3157 0.80 +0.4 161,477 + -0.1 161,586 + GFF3158 0.20 -0.0 162,047 - GFF3158 0.89 +1.3 162,047 - GFF3158 0.89 +0.3 162,051 + GFF3158 0.89 +0.2 162,052 - GFF3158 0.89 +0.1 162,052 - GFF3158 0.89 +1.7 162,056 - -0.4 162,285 + GFF3159 0.22 -1.6 162,318 + GFF3159 0.27 -0.3 162,347 - GFF3159 0.31 -0.4 162,370 + GFF3159 0.34 +0.5
Or see this region's nucleotide sequence