Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3081 and GFF3082 overlap by 8 nucleotides GFF3082 and GFF3083 are separated by 72 nucleotides GFF3083 and GFF3084 are separated by 14 nucleotides
GFF3081 - Unknown, probable amino acid transporter, at 90,547 to 92,046
GFF3081
GFF3082 - hypothetical protein, at 92,039 to 92,182
GFF3082
GFF3083 - FIG074102: hypothetical protein, at 92,255 to 92,629
GFF3083
GFF3084 - FIG00553873: hypothetical protein, at 92,644 to 93,945
GFF3084
Position (kb)
92
93 Strain fitness (log2 ratio)
-2
-1
0
1 at 91.296 kb on + strand, within GFF3081 at 91.296 kb on + strand, within GFF3081 at 91.296 kb on + strand, within GFF3081 at 91.297 kb on - strand, within GFF3081 at 91.297 kb on - strand, within GFF3081 at 91.297 kb on - strand, within GFF3081 at 91.424 kb on + strand, within GFF3081 at 91.577 kb on - strand, within GFF3081 at 91.641 kb on + strand, within GFF3081 at 91.641 kb on + strand, within GFF3081 at 91.641 kb on + strand, within GFF3081 at 91.642 kb on - strand, within GFF3081 at 91.800 kb on + strand, within GFF3081 at 91.831 kb on - strand, within GFF3081 at 91.878 kb on + strand, within GFF3081 at 91.878 kb on + strand, within GFF3081 at 91.891 kb on + strand, within GFF3081 at 92.041 kb on - strand at 92.076 kb on + strand, within GFF3082 at 92.101 kb on + strand, within GFF3082 at 92.101 kb on + strand, within GFF3082 at 92.101 kb on + strand, within GFF3082 at 92.102 kb on - strand, within GFF3082 at 92.105 kb on + strand, within GFF3082 at 92.120 kb on - strand, within GFF3082 at 92.123 kb on + strand, within GFF3082 at 92.123 kb on + strand, within GFF3082 at 92.124 kb on - strand, within GFF3082 at 92.124 kb on - strand, within GFF3082 at 92.124 kb on - strand, within GFF3082 at 92.210 kb on - strand at 92.492 kb on - strand, within GFF3083 at 92.495 kb on + strand, within GFF3083 at 92.495 kb on + strand, within GFF3083 at 92.495 kb on + strand, within GFF3083 at 92.496 kb on - strand, within GFF3083 at 92.630 kb on + strand at 92.630 kb on + strand at 92.630 kb on + strand at 92.631 kb on - strand at 92.707 kb on + strand at 92.708 kb on - strand at 92.786 kb on - strand, within GFF3084 at 92.810 kb on - strand, within GFF3084 at 92.960 kb on + strand, within GFF3084 at 93.015 kb on + strand, within GFF3084 at 93.160 kb on - strand, within GFF3084 at 93.259 kb on + strand, within GFF3084 at 93.600 kb on + strand, within GFF3084
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 91,296 + GFF3081 0.50 -0.7 91,296 + GFF3081 0.50 +0.1 91,296 + GFF3081 0.50 -0.5 91,297 - GFF3081 0.50 +0.4 91,297 - GFF3081 0.50 +0.3 91,297 - GFF3081 0.50 +1.1 91,424 + GFF3081 0.58 -0.2 91,577 - GFF3081 0.69 +0.3 91,641 + GFF3081 0.73 +0.1 91,641 + GFF3081 0.73 +0.6 91,641 + GFF3081 0.73 -0.0 91,642 - GFF3081 0.73 +0.3 91,800 + GFF3081 0.84 -0.2 91,831 - GFF3081 0.86 -0.6 91,878 + GFF3081 0.89 -0.1 91,878 + GFF3081 0.89 -0.4 91,891 + GFF3081 0.90 -0.1 92,041 - -0.4 92,076 + GFF3082 0.26 -0.3 92,101 + GFF3082 0.43 -0.5 92,101 + GFF3082 0.43 +0.1 92,101 + GFF3082 0.43 +0.9 92,102 - GFF3082 0.44 -0.5 92,105 + GFF3082 0.46 -0.1 92,120 - GFF3082 0.56 -0.0 92,123 + GFF3082 0.58 +0.8 92,123 + GFF3082 0.58 +0.4 92,124 - GFF3082 0.59 +0.3 92,124 - GFF3082 0.59 +0.2 92,124 - GFF3082 0.59 +0.5 92,210 - +0.4 92,492 - GFF3083 0.63 +0.2 92,495 + GFF3083 0.64 -0.7 92,495 + GFF3083 0.64 -1.9 92,495 + GFF3083 0.64 -0.7 92,496 - GFF3083 0.64 -0.2 92,630 + +0.7 92,630 + +0.1 92,630 + -1.1 92,631 - +0.2 92,707 + +0.2 92,708 - +0.3 92,786 - GFF3084 0.11 +0.4 92,810 - GFF3084 0.13 -0.1 92,960 + GFF3084 0.24 -0.4 93,015 + GFF3084 0.28 +0.9 93,160 - GFF3084 0.40 -0.9 93,259 + GFF3084 0.47 +0.5 93,600 + GFF3084 0.73 +0.3
Or see this region's nucleotide sequence