Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF303 and GFF304 are separated by 74 nucleotides GFF304 and GFF305 overlap by 1 nucleotides GFF305 and GFF306 are separated by 9 nucleotides
GFF303 - Putative uncharacterized protein STY3991, at 20,846 to 21,859
GFF303
GFF304 - Transcriptional regulatory protein UhpA, at 21,934 to 22,524
GFF304
GFF305 - Sensor histidine protein kinase UhpB, glucose-6-phosphate specific (EC 2.7.13.3), at 22,524 to 24,026
GFF305
GFF306 - Hexose phosphate uptake regulatory protein UhpC, at 24,036 to 25,364
GFF306
Position (kb) 22
23
24
25 Strain fitness (log2 ratio) -1
0
1 at 21.611 kb on + strand, within GFF303 at 21.714 kb on - strand, within GFF303 at 21.714 kb on - strand, within GFF303 at 21.899 kb on + strand at 22.077 kb on - strand, within GFF304 at 22.082 kb on + strand, within GFF304 at 22.082 kb on + strand, within GFF304 at 22.082 kb on + strand, within GFF304 at 22.082 kb on + strand, within GFF304 at 22.083 kb on - strand, within GFF304 at 22.096 kb on - strand, within GFF304 at 22.110 kb on + strand, within GFF304 at 22.233 kb on + strand, within GFF304 at 22.273 kb on - strand, within GFF304 at 22.273 kb on - strand, within GFF304 at 22.294 kb on + strand, within GFF304 at 22.295 kb on - strand, within GFF304 at 22.397 kb on + strand, within GFF304 at 22.522 kb on + strand at 22.523 kb on - strand at 22.676 kb on + strand, within GFF305 at 22.677 kb on - strand, within GFF305 at 22.737 kb on + strand, within GFF305 at 22.858 kb on + strand, within GFF305 at 23.005 kb on - strand, within GFF305 at 23.019 kb on - strand, within GFF305 at 23.153 kb on + strand, within GFF305 at 23.586 kb on + strand, within GFF305 at 23.587 kb on - strand, within GFF305 at 23.587 kb on - strand, within GFF305 at 23.632 kb on + strand, within GFF305 at 23.641 kb on + strand, within GFF305 at 23.642 kb on - strand, within GFF305 at 23.800 kb on + strand, within GFF305 at 24.019 kb on - strand at 24.173 kb on + strand, within GFF306 at 24.174 kb on - strand, within GFF306 at 24.268 kb on - strand, within GFF306 at 24.406 kb on + strand, within GFF306 at 24.407 kb on - strand, within GFF306 at 24.407 kb on - strand, within GFF306 at 24.555 kb on - strand, within GFF306 at 24.786 kb on + strand, within GFF306 at 24.862 kb on - strand, within GFF306 at 24.863 kb on + strand, within GFF306 at 24.863 kb on + strand, within GFF306 at 24.910 kb on + strand, within GFF306 at 24.910 kb on + strand, within GFF306
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 21,611 + GFF303 0.75 +0.2 21,714 - GFF303 0.86 +0.0 21,714 - GFF303 0.86 +0.1 21,899 + -0.3 22,077 - GFF304 0.24 +0.1 22,082 + GFF304 0.25 +0.6 22,082 + GFF304 0.25 -0.6 22,082 + GFF304 0.25 -0.2 22,082 + GFF304 0.25 +0.2 22,083 - GFF304 0.25 +0.1 22,096 - GFF304 0.27 +0.3 22,110 + GFF304 0.30 -0.3 22,233 + GFF304 0.51 -0.2 22,273 - GFF304 0.57 -0.0 22,273 - GFF304 0.57 +0.3 22,294 + GFF304 0.61 +0.2 22,295 - GFF304 0.61 -0.1 22,397 + GFF304 0.78 +0.0 22,522 + -0.0 22,523 - -1.6 22,676 + GFF305 0.10 +0.3 22,677 - GFF305 0.10 +0.1 22,737 + GFF305 0.14 +0.3 22,858 + GFF305 0.22 -0.2 23,005 - GFF305 0.32 +0.5 23,019 - GFF305 0.33 -0.1 23,153 + GFF305 0.42 +0.6 23,586 + GFF305 0.71 +0.7 23,587 - GFF305 0.71 +0.3 23,587 - GFF305 0.71 -0.4 23,632 + GFF305 0.74 +0.5 23,641 + GFF305 0.74 -0.1 23,642 - GFF305 0.74 +0.4 23,800 + GFF305 0.85 -0.1 24,019 - +0.4 24,173 + GFF306 0.10 -0.3 24,174 - GFF306 0.10 +0.0 24,268 - GFF306 0.17 +0.9 24,406 + GFF306 0.28 -0.0 24,407 - GFF306 0.28 -0.0 24,407 - GFF306 0.28 -0.3 24,555 - GFF306 0.39 +0.1 24,786 + GFF306 0.56 -0.6 24,862 - GFF306 0.62 -0.1 24,863 + GFF306 0.62 -1.1 24,863 + GFF306 0.62 -0.3 24,910 + GFF306 0.66 +0.8 24,910 + GFF306 0.66 +0.3
Or see this region's nucleotide sequence