Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3030 and GFF3031 are separated by 204 nucleotides GFF3031 and GFF3032 are separated by 279 nucleotides GFF3032 and GFF3033 are separated by 38 nucleotides
GFF3030 - Agmatinase (EC 3.5.3.11), at 44,653 to 45,573
GFF3030
GFF3031 - FIG01048344: hypothetical protein, at 45,778 to 46,668
GFF3031
GFF3032 - Putative mannitol dehydrogenase, at 46,948 to 47,421
GFF3032
GFF3033 - Malate dehydrogenase (EC 1.1.1.37), at 47,460 to 48,467
GFF3033
Position (kb) 45
46
47 Strain fitness (log2 ratio) -2
-1
0
1 at 44.897 kb on + strand, within GFF3030 at 44.941 kb on - strand, within GFF3030 at 45.071 kb on + strand, within GFF3030 at 45.110 kb on + strand, within GFF3030 at 45.219 kb on + strand, within GFF3030 at 45.220 kb on - strand, within GFF3030 at 45.297 kb on - strand, within GFF3030 at 45.297 kb on - strand, within GFF3030 at 45.667 kb on - strand at 45.707 kb on - strand at 45.711 kb on + strand at 45.760 kb on + strand at 45.779 kb on + strand at 45.827 kb on + strand at 45.925 kb on - strand, within GFF3031 at 45.970 kb on + strand, within GFF3031 at 45.970 kb on + strand, within GFF3031 at 46.105 kb on - strand, within GFF3031 at 46.205 kb on + strand, within GFF3031 at 46.205 kb on + strand, within GFF3031 at 46.258 kb on + strand, within GFF3031 at 46.387 kb on - strand, within GFF3031 at 46.387 kb on - strand, within GFF3031 at 47.022 kb on + strand, within GFF3032 at 47.078 kb on - strand, within GFF3032 at 47.149 kb on + strand, within GFF3032 at 47.421 kb on + strand at 47.583 kb on + strand, within GFF3033 at 47.593 kb on - strand, within GFF3033
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 44,897 + GFF3030 0.26 -0.5 44,941 - GFF3030 0.31 -0.0 45,071 + GFF3030 0.45 +0.1 45,110 + GFF3030 0.50 -0.5 45,219 + GFF3030 0.61 -0.3 45,220 - GFF3030 0.62 -0.5 45,297 - GFF3030 0.70 +1.0 45,297 - GFF3030 0.70 +0.0 45,667 - +0.5 45,707 - +0.2 45,711 + +0.5 45,760 + +0.3 45,779 + -0.1 45,827 + +0.1 45,925 - GFF3031 0.16 -1.3 45,970 + GFF3031 0.22 +0.5 45,970 + GFF3031 0.22 +0.2 46,105 - GFF3031 0.37 -0.8 46,205 + GFF3031 0.48 -2.1 46,205 + GFF3031 0.48 -0.2 46,258 + GFF3031 0.54 +0.1 46,387 - GFF3031 0.68 +0.2 46,387 - GFF3031 0.68 +0.4 47,022 + GFF3032 0.16 +0.0 47,078 - GFF3032 0.27 -0.0 47,149 + GFF3032 0.42 -0.1 47,421 + +0.1 47,583 + GFF3033 0.12 -0.6 47,593 - GFF3033 0.13 +0.3
Or see this region's nucleotide sequence